LOCUS       sequence-470142-       12645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express tagBFP-2A-coICP0-∆USP7 (ICP0 ∆618-633, co = 
            codon-optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12645)
  AUTHORS   Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, 
            Ait-Mohamed O, Maurin M, Waterfall JJ, Kaufer BB, Fischer P, Hennig 
            T, Dolken L, Lomonte P, Fachinetti D, Manel N
  TITLE     Centromeric DNA amplification triggered by viral proteins activates 
            nuclear cGAS.
  JOURNAL   Cell. 2025 May 29:S0092-8674(25)00556-2. doi: 
            10.1016/j.cell.2025.05.008.
  PUBMED    40460826
REFERENCE   2  (bases 1 to 12645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 29:S0092-8674(25)00556-2. doi: 10.1016/j.cell.2025.05.008."
COMMENT     Sequence Label: pTRIP-SFFV-tagBFP-2A-coICP0-∆USP7
FEATURES             Location/Qualifiers
     source          1..12645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             7049..7229
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8757..8776)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8990..9009)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9109..9131
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9169..9187)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9255..9359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9360..9428,9429..10220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9578..9597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10391..10979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10880..10899
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11133..11150
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11220..11240)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11225..11554
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11405..11540
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3781 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3901 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     4201 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
     4261 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc cactagtgtc
     4501 gacgagccta gacccggagc cagcacaaga agacccgagg gaagacccca aagagaaccc
     4561 gcccccgacg tgtgggtctt cccttgtgat agggacctcc ccgatagcag cgattccgaa
     4621 gctgagacag aggtgggagg aagaggcgac gctgaccacc acgatgacga ctccgctagc
     4681 gaggccgatt ccacagatac cgagctcttc gagaccggac tgctgggccc tcaaggcgtc
     4741 gatggaggag ctgtgtccgg cggcagccct cctagagagg aagaccccgg aagctgtgga
     4801 ggcgctcctc ccagagagga cggaggaagc gacgaaggag atgtgtgcgc cgtgtgcaca
     4861 gacgagatcg cccctcatct gaggtgtgac acctttcctt gcatgcatag attctgcatc
     4921 ccttgcatga agacatggat gcagctcaga aacacatgcc ctctgtgtaa cgctaaactg
     4981 gtgtatctga tcgtcggagt gacacctagc ggctccttta gcaccattcc tatcgtcaac
     5041 gacccccaaa caagaatgga agctgaagaa gctgtgaggg ccggcacagc cgtggatttc
     5101 atctggaccg gcaatcagag gtttgctcct agatatctga ccctcggagg acacacagtg
     5161 agggctctgt cccctacaca ccccgagcct acaacagacg aagacgacga cgacctcgat
     5221 gatgccgact acgtgccccc cgcccctagg agaacaccta gagctcctcc taggagaggc
     5281 gctgccgctc ctcccgtgac cggcggagcc agccatgctg cccctcaacc cgccgctgct
     5341 aggacagctc cccctagcgc tcccattgga ccccacggaa gcagcaacac aaacaccacc
     5401 accaatagct ccggaggcgg cggcagcaga caaagcagag ctgccgcccc tagaggagct
     5461 tccggaccta gcggaggagt cggagtcgga gtgggagtgg tggaggccga agccggaagg
     5521 cccagaggaa gaaccggacc tctggtgaac agacccgctc ctctcgctaa caatagagac
     5581 cccattgtga tcagcgacag cccccccgcc tcccctcata ggcctcccgc cgctcctatg
     5641 cccggcagcg cccctaggcc cggccctccc gccagcgctg ctgccagcgg acccgccaga
     5701 cctagggctg ctgtcgctcc ttgcgtgaga gcccctcctc ccggccccgg acctagggcc
     5761 cccgcccccg gagctgaacc cgccgccaga cccgccgatg ctagaagagt gccccagtcc
     5821 catagctctc tggctcaagc tgccaatcaa gagcaatctc tgtgcagagc tagggctaca
     5881 gtcgctagag gcagcggagg acccggcgtg gaaggcggac acggacctag cagaggagct
     5941 gctccttccg gagccgctcc tctgccttcc gccgctagcg tggagcaaga agccgccgtg
     6001 aggcctagaa agagaagggg ctccggccaa gagaacccca gccctcagtc cacaagacct
     6061 cccctcgctc ccgccggcgc taaaagagcc gccacacacc ctcctagcga ttccggcccc
     6121 ggaggcagag gccaaggcgg acccggaaca cctctgacat cctccgccgc ttccgctagc
     6181 tccagctccg ccagctccag cagcgctcct acacccgctg gagccgctag cagcgctgcc
     6241 ggagctgcta gcagcagcgc tagcgcttcc agcggaggag ctgtcggagc cctcggagga
     6301 agacaagagg aaacctctct gggacctaga gccgctagcg gccctagggg cgtgcccgcc
     6361 ggaggactga caaggtatct gcctatcagc ggagtcagca gcgtggtggc tctgagcccc
     6421 tacgtgaaca aaaccatcac cggagattgt ctgcccatcc tcgacatgga gaccggaaac
     6481 attggcgcct atgtggtgct ggtggatcag accggcaaca tggctaccag actgagagct
     6541 gccgtgcccg gctggtctag aaggacactc ctccccgaga cagccggaaa ccacgtgatg
     6601 cctcccgagt accccacagc ccccgctagc gaatggaata gcctctggat gacacccgtg
     6661 ggcaatatgc tgttcgacca aggaaccctc gtcggcgctc tggattttag atctctgagg
     6721 agcagacatc cttggtccgg cgagcaaggc gcttccacaa gagacgaggg aaagcagtga
     6781 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     6841 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     6901 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6961 aggggggact ggaagggcta attcactccc aacgaagaca aaatcgtcga gagatgctgc
     7021 atataagcag ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7081 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7141 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7201 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7261 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt ataatagatt
     7321 tagcaggaat tgaactagga gtggagcaca caggcaaagc tgcagaagta cttggaagaa
     7381 gccaccagag atactcacga ttctgcacat acctggctaa tcccagatcc taaggattac
     7441 attaagttta ctaacattta tataatgatt tatagtttaa agtataaact tatctaattt
     7501 actattctga cagatattaa ttaatcctca aatatcataa gagatgatta ctattatccc
     7561 catttaacac aagaggaaac tgagagggaa agatgttgaa gtaattttcc cacaattaca
     7621 gcatccgtta gttacgactc tatgatcttc tgacacaaat tccatttact cctcacccta
     7681 tgactcagtc gaatatatca aagttatgga cattatgcta agtaacaaat taccctttta
     7741 tatagtaaat actgagtaga ttgagagaag aaattgtttg caaacctgaa tagcttcaag
     7801 aagaagagaa gtgaggataa gaataacagt tgtcatttaa caagttttaa caagtaactt
     7861 ggttagaaag ggattcaaat gcataaagca agggataaat ttttctggca acaagactat
     7921 acaatataac cttaaatatg acttcaaata attgttggaa cttgataaaa ctaattaaat
     7981 attattgaag attatcaata ttataaatgt aatttacttt taaaaaggga acatagaaat
     8041 gtgtatcatt agagtagaaa acaatcctta ttatcacaat ttgtcaaaac aagtttgtta
     8101 ttaacacaag tagaatactg cattcaatta agttgactgc agattttgtg ttttgttaaa
     8161 attagaaaga gataacaaca atttgaatta ttgaaagtaa catgtaaata gttctacata
     8221 cgttcttttg acatcttgtt caatcattga tcgaagttct ttatcttgga agaatttgtt
     8281 ccaaagactc tgaaataagg aaaacaatct attatatagt ctcacacctt tgttttactt
     8341 ttagtgattt caatttaata atgtaaatgg ttaaaattta ttcttctctg agatcatttc
     8401 acattgcaga tagaaaacct gagactgggg taatttttat taaaatctaa tttaatctca
     8461 gaaacacatc tttattctaa catcaatttt tccagtttga tattatcata taaagtcagc
     8521 cttcctcatc tgcaggttcc acaacaaaaa tccaaccaac tgtggatcaa aaatattggg
     8581 aaaaaattaa aaatagcaat acaacaataa aaaaatacaa atcagaaaaa cagcacagta
     8641 taacaacttt atttagcatt tacaatctat taggtattat aagtaatcta gccagatcct
     8701 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     8761 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     8821 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     8881 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     8941 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     9001 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     9061 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     9121 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     9181 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     9241 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     9301 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9361 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9421 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9481 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9541 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9601 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9661 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9721 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9781 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9841 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9901 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9961 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10021 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10081 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10141 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10201 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    10261 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    10321 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10381 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10441 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10501 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10561 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10621 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10681 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10741 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10801 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10861 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10921 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10981 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    11041 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    11101 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    11161 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
    11221 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    11281 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    11341 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    11401 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    11461 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    11521 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
    11581 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
    11641 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
    11701 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
    11761 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
    11821 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
    11881 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
    11941 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
    12001 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
    12061 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
    12121 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
    12181 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
    12241 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
    12301 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
    12361 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
    12421 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
    12481 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
    12541 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
    12601 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtg
//
