LOCUS       sequence-47020-e         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2458
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             78..143
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             150..170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agaagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agctggctag caccatggac tacaaagacc atgacggtga ttataaagat catgacatcg
      121 attacaagga tgacgatgac aagatggccc ccaagaagaa gaggaaggtg ggcattcacc
      181 gcggggtacc tatggtggac ttgaggacac tcggttattc gcaacagcaa caggagaaaa
      241 tcaagcctaa ggtcaggagc accgtcgcgc aacaccacga ggcgcttgtg gggcatggct
      301 tcactcatgc gcatattgtc gcgctttcac agcaccctgc ggcgcttggg acggtggctg
      361 tcaaatacca agatatgatt gcggccctgc ccgaagccac gcacgaggca attgtagggg
      421 tcggtaaaca gtggtcggga gcgcgagcac ttgaggcgct gctgactgtg gcgggtgagc
      481 ttagggggcc tccgctccag ctcgacaccg ggcagctgct gaagatcgcg aagagagggg
      541 gagtaacagc ggtagaggca gtgcacgcct ggcgcaatgc gctcaccggg gcccccttga
      601 acctgacccc agaccaggta gtcgcaatcg cgaacaataa tgggggaaag caagccctgg
      661 aaaccgt
//
