LOCUS       sequence-47021-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Qin Y, Dittmer PJ, Park JG, Jansen KB, Palmer AE
  TITLE     Measuring steady-state and dynamic endoplasmic reticulum and Golgi 
            Zn2+ with genetically encoded sensors.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Epub 2011 Apr 
            18.
  PUBMED    21502528
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2011-05-3"; volume: "108(18)"; pages: 
            "7351-6. Epub 2011 Apr 18"
COMMENT     Sequence Label: pcDNA-ZapCY1
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(18..20,21..23,24..536)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(63..84)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(324..343)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     671..692
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtccgagctc ggatcccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
       61 tcctggtcga gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg
      121 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      181 ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct
      241 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      301 aggagcgtac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      361 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      421 gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg
      481 ccgacaagca gaagaacggc atcaaggccc acttcaagat ccgccacaac atcgaggacg
      541 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      601 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
      661 agcgcgatca catggtcctg ctggagttcg tgaccgccgc ccgcatgcat aaaaacaatg
      721 acttaaaatg caaatggaaa gaatgtcctg agtcttgtag ctcactattt gacctacaaa
      781 gacatctttt gaaggatcat gtctctcaag atttcaaaca ccctatggaa ccattagctt
      841 gtaattggga ggactgtgat ttccttggag atgatacatg
//
