LOCUS       sequence-470253-        7637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of HA-tagged GTSE1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Garcia-Vazquez N, Gonzalez-Robles TJ, Lane E, Spasskaya D, Zhang Q, 
            Kerzhnerman M, Jeong YT, Collu M, Simoneschi D, Ruggles KV, Rona G, 
            Pagano M, Kaisari S
  TITLE     Stabilization of GTSE1 by cyclin D1-CDK4/6 promotes cell 
            proliferation: relevance in cancer prognosis
  JOURNAL   bioRxiv 2024.06.26.600797
REFERENCE   2  (bases 1 to 7637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.06.26.600797"
COMMENT     Sequence Label: pcDNA3.1-HA-GTSE1
FEATURES             Location/Qualifiers
     source          1..7637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             670..696
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             670..696
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             676..702
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        complement(2956..2974)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2957..2974)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2994..3011)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3000..3224
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3270..3698
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3357..3376)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3567..3588
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3707..3727)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3712..4041
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3892..4027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3954..3973
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4108..4902
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4162..4181)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4772..4791
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5076..5197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5113..5132)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5167..5186
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5246..5262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5246..5262)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5259..5281)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5270..5286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5294..5300,5301..5318,5319..5324))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5339..5360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5477..5494)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5648..6236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5728..5747)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6407..7198,7199..7267))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7030..7049
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7268..7372)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7447..7466)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 accatggcct acccctacga cgtgcccgac tacgcctccc tcggatccat ggaaggaggc
      721 ggcggccgcg atgagccttc agcctgccgg gcaggggacg tgaacatgga tgaccctaag
      781 aaggaagaca ttcttctttt ggccgatgaa aaatttgact tcgatctttc attgtcttct
      841 tcgagtgcaa atgaagatga tgaagtcttc ttcggaccct ttggacataa agaaagatgt
      901 attgctgcca gcttggaatt aaataatccg gttcccgaac agcctccgtt gcccacatct
      961 gagagtccct ttgcctggag ccctctggcc ggggagaagt tcgtggaggt gtacaaagaa
     1021 gctcacttac tggctttaca cattgagagc agcagccgga accaggcagc ccaagctgcc
     1081 aagcctgaag accctcggag ccagggcgtg gaaagattca tacaggagtc aaaattaaaa
     1141 ataaacctct ttgagaaaga aaaggaaatg aagaaaagcc ccacgtctct taaaagggag
     1201 acatactacc tgtcagacag ccccttgctg gggccccctg tgggtgagcc tcggctcttg
     1261 gcctcctccc cggccctgcc cagctctggt gcccaggccc gcctcacccg ggcgccgggg
     1321 cctccgcact ctgctcatgc tttgcccagg gaatcatgca ctgctcatgc tgcaagtcag
     1381 gcagcgactc agaggaagcc cgggaccaaa ttgctgctgc ctcgagcggc ctctgttaga
     1441 ggaagaagca tccctggggc tgcggagaag cccaagaaag agattccagc tagtccttcc
     1501 aggacaaaaa tcccagctga gaaggaatcc caccgggatg ttctccctga caaacctgcc
     1561 ccgggtgctg tcaatgtgcc ggccgccgga agccacttgg gccagggcaa gcgggcgatc
     1621 cctgttccaa acaagttggg gctgaagaag accctgttaa aagcacccgg ctctaccagc
     1681 aatctcgcaa ggaagtcctc ctcggggcct gtttggagcg gggcatccag tgcgtgcaca
     1741 tccccagcag tgggcaaagc taaatcaagt gaatttgcaa gtattcctgc aaatagctcc
     1801 cggcctctgt caaacatcag caagtcaggc agaatgggac ccgccatgct gcggccagct
     1861 ctgcctgcag gccctgtggg ggcatcctcc tggcaggcca agcgggtcga tgtttctgag
     1921 ctggcagcgg agcagctcac ggcacccccc tcagcatccc ccacccaacc ccagactccg
     1981 gaaggtggcg gccagtggct gaactccagt tgcgcttggt cagaatcttc tcaattgaat
     2041 aagactagaa gtatcagacg gcgagattcc tgtctaaatt ccaagacaaa ggttatgcct
     2101 actcctacaa atcaatttaa aattcctaag ttttctattg gtgactcccc ggacagctca
     2161 acaccaaagc tttcgcgggc acagcggccg cagtcgtgca cgtcagttgg cagggtcact
     2221 gtccacagca ccccggttag acgctcatct gggccagcac cacaaagcct gctgagcgca
     2281 tggcgtgtgt cagccttgcc cacacccgcc agccggcgct gctctggcct tccaccgatg
     2341 acccccaaaa cgatgcccag ggccgtgggc tctcccctgt gtgtgccagc tcggagacgt
     2401 tcctctgagc cccgcaagaa ctctgcaatg agaactgaac caacaaggga gagcaacaga
     2461 aagacagatt ccaggctggt ggatgtgtcc cctgacaggg gttctcctcc ttcccgtgtg
     2521 cctcaggcac ttaacttttc tccagaggaa agcgattcta ctttctccaa aagtactgcc
     2581 acagaagtag ctcgggagga agccaagccg ggtggagatg cagcccctag tgaggctctt
     2641 cttgtagata tcaaactgga accactcgcg gtcactccag atgctgcaag ccagcccctc
     2701 attgaccttc ctctcatcga cttctgcgat accccagaag cacacgtggc tgtaggatct
     2761 gaaagcaggc ctctgatcga cctcatgaca aacactccag acatgaataa aaatgtggcc
     2821 aaaccttcac cggtggtggg acagctcata gacctgagct cccctctgat ccagctgagc
     2881 cctgaggctg acaaggagaa cgtggattcc ccactcctca agttctaagt cgagcatgca
     2941 tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac
     3001 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     3061 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     3121 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     3181 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     3241 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     3301 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3361 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3421 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3481 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3541 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3601 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     3661 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     3721 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3781 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3841 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3901 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3961 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     4021 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     4081 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     4141 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     4201 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     4261 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     4321 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     4381 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     4441 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     4501 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     4561 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     4621 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     4681 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     4741 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     4801 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     4861 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     4921 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     4981 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     5041 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     5101 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5161 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     5221 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5281 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5341 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5401 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5461 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5521 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5581 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5641 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5701 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5761 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5821 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5881 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5941 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6001 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6061 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6121 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6181 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6241 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6301 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6361 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6421 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6481 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6541 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6601 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6661 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6721 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6781 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6841 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6901 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6961 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7021 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7081 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7141 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7201 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7261 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7321 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7381 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     7441 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     7501 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     7561 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtatg
     7621 ggccagatat acgcgtt
//
