LOCUS       sequence-470256-       11093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of the tetracycline 
            transactivator tTA and of the blue fluorescent protein tagBFP, 
            separated by an internal ribosome entry site (SFFV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11093)
  TITLE     Morsut lab plasmids 2024
REFERENCE   2  (bases 1 to 11093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR_SFFV_tTA_IRES_tagBFP
FEATURES             Location/Qualifiers
     source          1..11093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    339..464
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    961..1194
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1379..1423
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1708..1825
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1978..2385
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(2459..3076,3077..3082,3083..3118,3119..3121,3122..3157,
                     3158..3160,3161..3196,3197..3205)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3227..3799
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3393..3410)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3620..3639
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3818..4519
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    4615..5203
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4668..4688)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5086..5097)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5206..5222)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5207..5223)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5313..5546
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5571..5589)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5572..5589)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5879..5898)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6112..6131)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6231..6253
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6291..6309)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6377..6481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6482..6550,6551..7342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6700..6719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7513..8101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8002..8021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8255..8272
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8342..8362)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8347..8676
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8527..8662
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9810..9875
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10005..10025
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10450..10584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10487..10506)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10541..10560
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10752..11093,1..292)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
       61 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
      121 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      181 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      241 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      301 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      361 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      421 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      481 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      541 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      601 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      661 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      721 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      781 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
      841 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
      901 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
      961 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1021 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1081 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1141 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1201 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1261 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1321 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1381 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1441 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1501 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1561 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1621 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     1681 tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     1741 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     1801 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     1861 gatcgataag cttgatatcg aattcctgca gccccgataa aataaaagat tttatttagt
     1921 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agctgcagta
     1981 acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
     2041 gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg agcagtttcg
     2101 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
     2161 gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
     2221 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
     2281 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
     2341 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga tctggagctc
     2401 tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatcg gagctaccat
     2461 gtctagactg gacaagagca aagtcataaa ctctgctctg gaattactca atgaagtcgg
     2521 tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct
     2581 gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctggcaatcg agatgctgga
     2641 caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa
     2701 caacgccaag tcattccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct
     2761 cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg
     2821 tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac
     2881 actgggctgc gtattggagg atcaggagca tcaagtagca aaagaggaaa gagagacacc
     2941 taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg accatcaggg
     3001 agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct
     3061 aaagtgcgaa agcggcgggc cggccgacgc ccttgacgat tttgacttag acatgctccc
     3121 agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt
     3181 tgaccttgac atgctccccg ggtaatagga tccttgactt gcggcccccc tctccctccc
     3241 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     3301 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     3361 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     3421 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     3481 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     3541 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     3601 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     3661 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     3721 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     3781 ttgaaaaaca cgatgataaa tgagcgctgg cgggtccatg agcgagctga ttaaggagaa
     3841 catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc
     3901 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
     3961 cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac
     4021 cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt
     4081 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     4141 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc
     4201 caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta
     4261 ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag
     4321 ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta agaacctcaa
     4381 gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga
     4441 gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact
     4501 ggggcacaag cttaattaag gccgcaactc ccacctgcaa catgcgtgac tgactgaggc
     4561 cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat cgataatcaa
     4621 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4681 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4741 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4801 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4861 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4921 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4981 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     5041 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     5101 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5161 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     5221 cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     5281 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5341 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5401 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5461 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5521 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
     5581 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     5641 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     5701 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     5761 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     5821 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     5881 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     5941 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     6001 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     6061 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
     6121 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     6181 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     6241 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     6301 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     6361 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6421 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6481 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6541 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6601 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6661 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6721 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6781 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6841 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6901 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6961 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7021 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7081 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7141 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7201 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7261 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7321 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7381 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7441 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7501 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7561 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7621 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7681 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7741 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7801 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7861 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7921 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7981 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8041 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8101 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8161 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8221 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8281 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8341 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8401 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8461 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     8521 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8581 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8641 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg
     8701 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     8761 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     8821 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     8881 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     8941 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg
     9001 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
     9061 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
     9121 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
     9181 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
     9241 gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc
     9301 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
     9361 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
     9421 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
     9481 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
     9541 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
     9601 cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga
     9661 acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta
     9721 tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     9781 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     9841 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     9901 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
     9961 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
    10021 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
    10081 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
    10141 tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat aacagttata
    10201 atcataacat actgtttttt cttactccac acaggcatag agtgtctgct attaataact
    10261 atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt
    10321 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
    10381 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
    10441 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10501 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    10561 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
    10621 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
    10681 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
    10741 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
    10801 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
    10861 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
    10921 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
    10981 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
    11041 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag ctt
//
