LOCUS       sequence-470260-       10291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of the far-red 
            fluorescent protein miRFP703 fused with the H2B nuclear localization
            tag (SFFV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10291)
  TITLE     Morsut lab plasmids 2024
REFERENCE   2  (bases 1 to 10291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR_SFFV_H2B_miRFP703
FEATURES             Location/Qualifiers
     source          1..10291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             60..693
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    740..865
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1362..1595
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1780..1824
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2109..2226
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2379..2786
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2833..3210
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      3223..3232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3229..4176
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    4214..4802
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4267..4287)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4685..4696)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4805..4821)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4806..4822)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4912..5145
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5170..5188)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5171..5188)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5478..5497)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5711..5730)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5830..5852
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5890..5908)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5976..6080
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6081..6149,6150..6941)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6299..6318)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7112..7700
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7601..7620
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7854..7871
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7941..7961)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7946..8275
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8126..8261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9409..9474
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9604..9624
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10049..10183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10086..10105)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10140..10159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
       61 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
      121 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      181 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      241 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      301 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      361 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      421 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      481 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      541 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      601 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      661 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      721 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      781 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      841 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      901 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      961 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     1021 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1081 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1141 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1201 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1261 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1321 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1381 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1441 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1501 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1561 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1621 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1681 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1741 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1801 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1861 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1921 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1981 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2041 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     2101 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2161 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2221 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     2281 gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     2341 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat
     2401 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
     2461 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
     2521 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
     2581 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
     2641 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
     2701 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
     2761 tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcggc
     2821 gatcgcacgc gtatgccaga gccagcgaag tctgctcccg ccccgaaaaa gggctccaag
     2881 aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag ccgcaaggag
     2941 agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac cggcatttcg
     3001 tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg catcgcaggt
     3061 gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag ggagatccag
     3121 acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc cgagggtact
     3181 aaggccatca ccaagtacac cagcgctaag gatccaccgg tcgccaccat ggtagcaggt
     3241 catgcctctg gcagccccgc attcgggacc gcctctcatt cgaattgcga acatgaagag
     3301 atccacctcg ccggctcgat ccagccgcat ggcgcgcttc tggtcgtcag cgaacatgat
     3361 catcgcgtca tccaggccag cgccaacgcc gcggaatttc tgaatctcgg aagcgtactc
     3421 ggcgttccgc tcgccgagat cgacggcgat ctgttgatca agatcctgcc gcatctcgat
     3481 cccaccgccg aaggcatgcc ggtcgcggtg cgctgccgga tcggcaatcc ctctacggag
     3541 tactgcggtc tgatgcatcg gcctccggaa ggcgggctga tcatcgaact cgaacgtgcc
     3601 ggcccgtcga tcgatctgtc aggcacgctg gcgccggcgc tggagcggat ccgcacggcg
     3661 ggttcactgc gcgcgctgtg cgatgacacc gtgctgctgt ttcagcagtg caccggctac
     3721 gaccgggtga tggtgtatcg tttcgatgag caaggccacg gcctggtatt ctccgagtgc
     3781 catgtgcctg ggctcgaatc ctatttcggc aaccgctatc cgtcgtcgct ggtcccgcag
     3841 atggcgcggc agctgtacgt gcggcagcgc gtccgcgtgc tggtcgacgt cacctatcag
     3901 ccggtgccgc tggagccgcg gctgtcgccg ctgaccgggc gcgatctcga catgtcgggc
     3961 tgcttcctgc gctcgatgtc gccgatccat ctgcagttcc tgaaggacat gggcgtgcgc
     4021 gccaccctgg cggtgtcgct ggtggtcggc ggcaagctgt ggggcctggt tgtctgtcac
     4081 cattatctgc cgcgcttcat ccgtttcgag ctgcgggcga tctgcaaacg gctcgccgaa
     4141 aggatcgcga cgcggatcac cgcgcttgag agctaacctg caggcatgca agcttgatat
     4201 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4261 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4321 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4381 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4441 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4501 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4561 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     4621 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4681 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4741 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4801 gcatcgatac cgtcgacctc gagggaatta attcgagctc ggtaccttta agaccaatga
     4861 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     4921 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     4981 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5041 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5101 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat
     5161 tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta
     5221 attgtagccg cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact
     5281 gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac
     5341 cttctgagtt tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag
     5401 ggtcatcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     5461 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     5521 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     5581 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     5641 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt
     5701 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     5761 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     5821 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     5881 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     5941 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6001 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6061 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6121 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6181 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6241 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6301 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6361 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6421 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6481 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6541 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6601 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6661 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6721 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6781 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6841 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6901 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6961 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7021 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7081 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7141 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7201 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7261 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7321 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7381 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7441 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7501 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7561 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7621 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7681 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7741 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7801 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7861 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7921 cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7981 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8041 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8101 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8161 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8221 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8281 tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
     8341 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
     8401 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     8461 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     8521 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     8581 gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     8641 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     8701 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     8761 tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     8821 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
     8881 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     8941 acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag
     9001 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     9061 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     9121 cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     9181 gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
     9241 ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc
     9301 gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
     9361 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     9421 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     9481 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     9541 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     9601 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     9661 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     9721 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
     9781 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
     9841 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
     9901 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
     9961 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    10021 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    10081 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10141 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    10201 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    10261 gtttcattta ctctaaacct gtgattcctc t
//
