LOCUS       sequence-470261-       10769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of the red fluorescent 
            protein mCherry (TRE3G promoter) and constitutive expression of the 
            blue fluorescent protein tagBFP (PGK promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10769)
  TITLE     Morsut lab plasmids 2024
REFERENCE   2  (bases 1 to 10769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR_TRE3G_mCherry_WPRE_PGK_tagBFP
FEATURES             Location/Qualifiers
     source          1..10769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        226..244
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     226..243
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             269..502
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(644..1345)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        complement(1366..1853)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1414..1433)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     1795..1818
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    1870..2458
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1976..1987
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2385..2405
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2496..3206)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2774..2793)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3043..3061
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(3255..3604)
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     primer_bind     complement(3292..3316)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3362..3380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3398..3416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3434..3452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3470..3488
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3506..3524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3542..3560
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3578..3596
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    3666..3783
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4068..4112)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4297..4530
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5027..5152
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5199..5832
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6000..6134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6024..6043)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6078..6097
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6559..6579)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6709..6774
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(7908..8237)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7922..8057
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8222..8242
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(8312..8329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8483..9071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8563..8582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9242..10033,10034..10102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9865..9884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10103..10207)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10275..10293
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10331..10353)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10453..10472
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10686..10705
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gctagcgatg accctgctga ttggttcgct gaccatttcc gggtgcggga cggcgttacc
       61 agaaactcag aaggttcgtc caaccaaacc gactctgacg gcagtttacg agagagatga
      121 tagggtctgc ttcagtaagc cagatgctac acaattaggc ttgtacatat tgtcgttaga
      181 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
      241 tagaatacac ggaattaatt ctagatgctg ctagagattt tccacactga ctaaaagggt
      301 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
      361 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
      421 gctcagatct ggtctaacca gagagaccca gtacaagcaa aaagcagatc ttgtcttcgt
      481 tgggagtgaa ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca
      541 gctgccttgt aagtcattgg tcttaaaggt accgagctcg aattaattcc ctcgaggtcg
      601 acaaataaat cgataagctt gatatcaagc ttgcatgcct gcattaatta agcttgtgcc
      661 ccagtttgct agggaggtcg cagtatctgg ccactgccac ctcgtgctgc tcgacgtagg
      721 tctcgttgtt ggcctccttg attctttcca gtctgtagtc cacatagtag acgccaggca
      781 tcttgaggtt cttagcgggt ttcttggatc tatatgtggt cttgatgttt gcgatcagat
      841 ggctcccgcc cacgagcttc agggccatgt cgtttctgcc ttccaggccg ccgtcagcgg
      901 ggtacagcgt ctcggtgaag gcctcccagc cgagtgtttt cttctgcatc acagggccgt
      961 tggatgtgaa gttcacccct ctgatcttga cgttgtagat gaggcagccg tcctggaggc
     1021 tggtgtcctg ggtagcggtc agcacgcccc cgtcttcgta tgtggtgact ctctcccatg
     1081 tgaagccctc agggaaggac tgcttgaaga agtcggggat gccctgggtg tggttgatga
     1141 aggtcttgct gccgtagagg aagctagtag ccaggatgtc gaaggcgaag gggagagggc
     1201 cgccctcgac caccttgatt ctcatggtct gggtgccctc gtagggcttg ccttcgccct
     1261 cggatgtgca cttgaagtga tggttgtcca cggtgccctc catgtacagc ttcatgtgca
     1321 tgttctcctt aatcagctcg ctcatggtaa gcttgggctg caggtcgaaa ggcccggaga
     1381 tgaggaagag gagaacagcg cggcagacgt gcgcttttga agcgtgcaga atgccgggcc
     1441 tccggaggac cttcgggcgc ccgccccgcc cctgagcccg cccccggacc caccccttcc
     1501 cagcctctga gcccagaaag cgaaggagca aagctgctat tggccgctgc cccaaaggcc
     1561 tacccgcttc cattgctcag cggtgctgtc catctgcacg agactagtga gacgtgctac
     1621 ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg ggggggaact tcctgactag
     1681 gggaggagta gaaggtggcg cgaaggggcc accaaagaac ggagccggtt ggcgcctacc
     1741 ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg tagcgccaag tgcccagcgg
     1801 ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc gcctccccta cccggtcgac
     1861 ggtatcgatg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa
     1921 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga
     1981 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga
     2041 gcagccaagg aaaggacgat gatttccccg acaacaccac ggaattgtca gtgcccaaca
     2101 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag
     2161 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag
     2221 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca
     2281 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac
     2341 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag
     2401 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattat
     2461 cgataagctt gatatcaagc ttgcatgcct gcaggttact tgtacagctc gtccatgccg
     2521 ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg
     2581 tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc
     2641 ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc
     2701 ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc
     2761 tcccagccca tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc
     2821 agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc
     2881 acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc
     2941 ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac
     3001 tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg
     3061 gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg
     3121 ccgttcacgg agccctccat gtgcaccttg aagcgcatga actccttgat gatggccatg
     3181 ttatcctcct cgcccttgct caccatcccg ggcccgcggt accgcaagct tgatatcctg
     3241 cagacgcgac gcgtaagtat aagacaaaag tgttgtggaa ttgctccagg cgatctgacg
     3301 gttcactaaa cgagctctgc ttttataggc gcccaccgta cacgcctacc tcgacatacg
     3361 ttctctatca ctgataggga gtaaactgga tatacgttct ctatcactga tagggagtaa
     3421 actgtagata cgttctctat cactgatagg gagtaaactg gtcatacgtt ctctatcact
     3481 gatagggagt aaactcctta tacgttctct atcactgata gggagtaaag tctgcatacg
     3541 ttctctatca ctgataggga gtaaactctt catacgttct ctatcactga tagggagtaa
     3601 actcgagtga agacgaaagg gcctcatcga taaactggat ctctgctgtc cctgtaataa
     3661 acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
     3721 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
     3781 aaattgtgga tgaatactgc catttggcga taccgtcgag atccgttcac taatcgaatg
     3841 gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc
     3901 ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc
     3961 actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg
     4021 aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat
     4081 ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt
     4141 aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg
     4201 tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg
     4261 gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat
     4321 ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac
     4381 agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac
     4441 aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct
     4501 gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc
     4561 actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat
     4621 ttatataatt cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagc
     4681 ggccggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta
     4741 aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg
     4801 tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca
     4861 gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta
     4921 gctccctgct tgcccatact atatgtttta atttatattt tttctttccc cctggcctta
     4981 accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc
     5041 catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc
     5101 cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg
     5161 gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga
     5221 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     5281 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     5341 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
     5401 cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
     5461 gaaagtcccc agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact
     5521 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     5581 acacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt
     5641 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
     5701 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga
     5761 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttctt tgggagtgaa
     5821 ttagcccttc caactactaa gtttgtagta catatttaac aaatacaatt tctttaaaat
     5881 gaaaataatt cagaggaatc acaggtttag agtaaatgaa accacaggta attggcagtg
     5941 gtaatagggt atggggtggg aagtttggga tgattttggt tagcttgagt tatccagttg
     6001 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     6061 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     6121 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     6181 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     6241 tctagtcaag gcactataca tcaaatattc cttattaacc cctttacaaa ttaaaaagct
     6301 aaaggtacac aatttttgag catagttatt aatagcagac actctatgcc tgtgtggagt
     6361 aagaaaaaac agtatgttat gattataact gttatgccta cttataaagg ttacagaata
     6421 tttttccata attttcttgt atagcagtgc agctttttcc tttgtggtgt aaatagcaaa
     6481 gcaagcaaga gttctattac taaacacagc atgactcaaa aaacttagca attctgaagg
     6541 aaagtccttg gggtcttcta cctttctctt cttttttgga ggagtagaat gttgagagtc
     6601 agcagtagcc tcatcatcac tagatggcat ttcttctgag caaaacaggt tttcctcatt
     6661 aaaggcattc caccactgct cccattcatc agttccatag gttggaatct aaaatacaca
     6721 aacaattaga atcagtagtt taacacatta tacacttaaa aattttatat ttaccttaga
     6781 gctttaaatc tctgtaggta gtttgtccaa ttatgtcaca ccacagaagt aaggttcctt
     6841 cacaaagatc cggggcccac tcataaatcc agttgccgcc acggtagcca atcaccgtat
     6901 cgtataaatc atcgtcggta cgttcggcat cgctcatcac aatacgtgcc tggacgtcga
     6961 ggatttcgcg tgggtcaatg ccgcgccaga tccacatcag acggttaatc atgcgatacc
     7021 agtgagggat ggttttacca tcaagggccg actgcacagg cggttgtgcg ccgtgattaa
     7081 agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac agggtttcgc
     7141 tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac tattgtaacc
     7201 cgcctgaagt taaaaagaac aacgcccggc agtgccaggc gttgaaaaga ttagcgaccg
     7261 gagattggcg ggacgaatac gacgcccata tcccacggct gttcaatcca ggtatcttgc
     7321 gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc gaagatggtg
     7381 acaaagtgcg cttttggata catttcacga atcgcaaccg cagtaccacc ggtatccacc
     7441 aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag cactttaagc
     7501 tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg acgaataccc
     7561 agttcacgcg ccagtaacgc acccggtaca gaccgccacg gcttacggca ataatgcctt
     7621 tccattgttc agaaggcatc agtcggcttg cgagtttacg tgcatggatc tgcaacatgt
     7681 cccaggtgac gatgtatttt tcgctcatgt gaagtgtccc agcctgttta tctacggctt
     7741 aaaaagtgtt cgaggggaaa ataggttgcg cgagattata gagatctggc gcactaaaaa
     7801 ccagtatttc acatgagtcc gcgtcttttt acgcactgcc tctccctgac gcgggataaa
     7861 gtggtattct caaacatatc tcgcaagcct gtcttgtgtc caagcttttt gcaaaagcct
     7921 aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg
     7981 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
     8041 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga
     8101 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct
     8161 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     8221 caccctaact gacacacatt ccacagctgc attaatgaat cggccaacgc gcggggagag
     8281 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8341 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8401 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8461 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8521 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8581 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8641 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8701 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8761 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8821 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8881 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8941 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9001 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9061 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9121 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9181 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9241 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9301 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9361 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9421 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9481 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9541 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9601 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9661 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9721 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9781 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9841 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9901 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9961 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10021 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10081 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10141 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10201 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
    10261 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
    10321 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
    10381 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
    10441 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccattcg acgctctccc
    10501 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
    10561 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
    10621 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
    10681 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
    10741 gccacgatgc gtccggcgta gaggatctg
//
