LOCUS       sequence-470264-       11427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CFP tagged AMOT gene in mammalian cells using the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Wang L, Choi K, Su T, Li B, Wu X, Zhang R, Driskill JH, Li H, Lei H,
            Guo P, Chen EH, Zheng Y, Pan D
  TITLE     Multiphase coalescence mediates Hippo pathway activation.
  JOURNAL   Cell. 2022 Nov 10;185(23):4376-4393.e18. doi: 
            10.1016/j.cell.2022.09.036. Epub 2022 Oct 31.
  PUBMED    36318920
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.09.036"; journalName: "Cell"; date: 
            "2022-11-10- 10"; volume: "185"; issue: "23"; pages: "4376-4393.e18"
COMMENT     Sequence Label: pCDH-CMV-FLAG-CFP-AMOT
FEATURES             Location/Qualifiers
     source          1..11427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        253..464
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             477..745
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             772..1371
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(772..791)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1268..1288
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1378..1966
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1431..1451)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1849..1860)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2040..2273
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2345..2466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2382..2401)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2436..2455
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2506..2641
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2674..2690)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2674..2690)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2687..2709)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2698..2714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2722..2728,2729..2746,2747..2752))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2767..2788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2905..2922)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3076..3664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(3835..4626,4627..4695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4458..4477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4696..4800)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4868..4886
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4924..4946)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5046..5065
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5259..5281
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5273..5290
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5274..5290
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5305..5531
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5532..5712
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5759..5884
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6377..6610
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6795..6839
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7106..7222
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7312..7515
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7466..7486
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7512..7536
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7601..7624
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(7649..7651,7652..8362)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7691..7712)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7952..7971)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8299..8320
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             9536..9556
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             11086..11103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tccaactcca actccagctg tggctcaggc tgaggttcct gcaagtccag ctaccggtcc
       61 tggaccacat cgtttgtcta taccaagttt gacctgcaat ccagacaaaa cagatgggcc
      121 tgtgttccac tccaatactc tggaaagaaa aactcccatt cagatcctgg gacaagagcc
      181 tgatgcagag atggtggaat atctcatcta agcggccgcg aaggatctgc gatcgctccg
      241 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      301 tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      361 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      421 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg
      481 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga
      541 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa
      601 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac
      661 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt
      721 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta gatgaccgag
      781 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
      841 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
      901 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
      961 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     1021 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     1081 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     1141 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     1201 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     1261 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     1321 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat
     1381 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     1441 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     1501 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     1561 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     1621 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     1681 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     1741 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     1801 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     1861 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     1921 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     1981 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     2041 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
     2101 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     2161 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     2221 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     2281 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     2341 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     2401 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2461 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     2521 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     2581 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     2641 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     2701 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     2761 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     2821 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2881 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3001 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3061 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3121 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3181 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3241 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3301 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3361 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3421 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3481 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3541 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3601 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3661 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3721 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3781 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3841 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3901 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3961 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4021 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4081 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4141 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4201 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4261 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4321 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4381 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4441 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4501 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4561 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4621 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4681 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4741 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4801 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     4861 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     4921 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     4981 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     5041 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     5101 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     5161 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     5221 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     5281 gacggccagt gccaagctga cgcgtgtagt cttatgcaat actcttgtag tcttgcaaca
     5341 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     5401 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     5461 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     5521 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5581 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5641 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5701 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     5761 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     5821 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     5881 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     5941 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     6001 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     6061 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     6121 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     6181 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccactg atcttcagac
     6241 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     6301 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     6361 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     6421 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     6481 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     6541 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     6601 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     6661 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     6721 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     6781 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     6841 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     6901 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     6961 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     7021 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     7081 acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg
     7141 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     7201 aaattacaaa attcaaaatt ttatcgatac tagtattatg cccagtacat gaccttatgg
     7261 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     7321 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     7381 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     7441 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtt
     7501 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
     7561 tttgacctcc atagaagatt ctagagctag cgaattcatg gattacaagg atgatgatga
     7621 taagggcggt gggtcgggtg gaggctcagt gagcaagggc gaggagctgt tcaccggggt
     7681 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     7741 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     7801 caagctgccc gtgccctggc ccaccctcgt gaccaccctg tcctggggcg tgcagtgctt
     7861 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     7921 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     7981 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     8041 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tactttagcg acaacgtcta
     8101 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     8161 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     8221 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     8281 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     8341 cggcatggac gagctgtaca agggtggagg ctcggggggt ggctcgagaa attctgaaga
     8401 acagccaagt ggagggacca cggtattgca gcgtttgcta caagagcagc ttcgctatgg
     8461 caatcctagt gagaatcgca gcctgcttgc catacaccag caagccacag ggaatggccc
     8521 tcctttcccc agtggcagtg ggaacccggg ccctcagagt gatgtgttga gtccccaaga
     8581 ccaccaccaa cagcttgtgg ctcatgctgc tcgacaagaa ccccaggggc aggaaatcca
     8641 gtcagaaaac ctcatcatgg agaagcagct gtctcctcga atgcaaaata atgaagaact
     8701 cccgacctat gaagaagcca aggtccagtc ccagtacttt cggggccaac agcatgccag
     8761 tgttggagct gccttctatg tcactggagt caccaaccag aagatgagga ctgagggacg
     8821 cccatcagtt cagcggctca atcctggaaa gatgcaccaa gatgagggac tcagagacct
     8881 taagcaaggg catgtccgtt ccttgagtga acgactaatg cagatgtcac tggccaccag
     8941 tggagttaag gcccatccac ctgttaccag tgctcccctc tccccaccac aacccaatga
     9001 cctctacaag aatcccacaa gttccagtga attctacaag gcccaagggc cacttcctaa
     9061 ccagcatagc ctgaagggca tggaacaccg aggcccccca ccagaatatc ccttcaaggg
     9121 catgccaccc caatctgtag tgtgcaagcc ccaagagcca gggcacttct atagtgagca
     9181 tcgcctgaac cagccaggga gaacagaggg gcaactgatg aggtatcagc atccccctga
     9241 gtatggagca gccaggccag cgcaggacat ctcattgcca ttgtcagcca ggaactcgca
     9301 gcctcacagc cctacttctt ctctgacctc tggggggtcc ttgcccttgc tacaatctcc
     9361 accatccact agattgtctc ctgcccgaca ccccttggtc ccaaaccagg gagaccattc
     9421 agctcacctg cctaggccgc agcagcattt ccttcctaat caggctcacc agggggatca
     9481 ttaccgtctc tcccaacctg gcctgagtca gcagcagcag caacagcagc agcagcacca
     9541 tcatcaccat caccaccaac aacagcagca gcagcagcca cagcagcagc caggagaagc
     9601 ctattcagct atgcctcggg ctcagccatc ctctgcttct tatcagccag tgccagcaga
     9661 cccttttgcc attgtttcca gagcccagca gatggttgag atcctctcag acgagaaccg
     9721 gaacttgagg caagagttgg aaggatgcta tgagaaggtg gcaagactgc agaaggtgga
     9781 gacagaaatc cagcgcgtct cggaggcata tgagaacctc gtgaagtcat cctccaaaag
     9841 agaggcccta gagaaagcca tgagaaacaa gctagagggc gagattcgga ggatgcatga
     9901 tttcaacagg gatctgagag agcgtctaga gactgccaac aagcagcttg cagagaagga
     9961 atatgagggg tcagaggaca ccagaaaaac catctcgcag ctctttgcaa aaaataaaga
    10021 aagccagcgt gagaaggaga agctggaagc ggagctggcc actgcccgtt ctaccaatga
    10081 ggaccaaaga cgacacatcg aaatccgaga tcaggccctg agtaatgccc aggccaaggt
    10141 ggtaaagctg gaagaagagc tgaaaaagaa gcaagtgtac gttgacaagg tggagaagat
    10201 gcagcaggcc cttgtacagc tccaggcagc atgtgaaaaa cgtgagcagc tagagcaccg
    10261 tctccggaca cgactggaga gggaactgga atccctgaga atccagcagc gtcagggcaa
    10321 ctgtcagccc accaacgttt cagaatacaa tgctgctgca ctgatggagc tccttcggga
    10381 gaaagaggag aggattctgg ctctggaagc tgatatgaca aagtgggagc agaaatattt
    10441 ggaggagaat gtgatgagac attttgctct ggatgctgct gcaactgtgg ctgctcagag
    10501 ggacacaaca gtcatcagtc actctcctaa caccagctat gacacagctc tagaagctcg
    10561 catccagaaa gaggaggaag aaatcttgat ggccaataag cgttgccttg acatggaggg
    10621 caggattaag accctccatg cccagattat tgagaaggat gccatgatca aagtactcca
    10681 gcagcgttcc cggaaggagc cgagcaagac agagcagctg tcgtgcatgc ggccagcgaa
    10741 gtctctgatg tccatttcca atgctggatc aggcttgctc tcccactcat ccaccctgac
    10801 tggctccccc atcatggaag aaaagcgaga cgacaagagc tggaagggga gcctaggcat
    10861 tctcctgggt ggagactacc gtgctgaata tgtcccttcc acaccctcgc ctgtgccacc
    10921 ctcgactccc ctgctctcgg ctcactccaa gacagacagc cgagactgca gtacccaaac
    10981 tgaacgtggg acggaatcga acaaaactgc agctgttgct cccatctctg ttcctgctcc
    11041 agttgctgct gccgccactg ctgccgccat cactgccact gctgccacca tcaccaccac
    11101 catggtagct gctgctccag ttgctgttgc tgctgctgct gctccagctg ctgctgctgc
    11161 cccgtctcca gccactgccg ctgctactgc tgctgctgtt tctccagctg ctgctggtca
    11221 gattccagct gctgcctctg ttgcctcagc tgctgccgtt gctccttctg ctgctgctgc
    11281 tgctgctgtt caggttgctc cagctgctcc ggctccagtt ccagctccgg ctctggttcc
    11341 ggtcccagct ccagcagcgg ctcaggcttc tgctcctgct cagactcagg caccaacttc
    11401 agctccggct gtggctccaa ctccagc
//
