LOCUS       sequence-470269-        9645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express RFP tagged MST2 gene in mammalian cells using the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Wang L, Choi K, Su T, Li B, Wu X, Zhang R, Driskill JH, Li H, Lei H,
            Guo P, Chen EH, Zheng Y, Pan D
  TITLE     Multiphase coalescence mediates Hippo pathway activation.
  JOURNAL   Cell. 2022 Nov 10;185(23):4376-4393.e18. doi: 
            10.1016/j.cell.2022.09.036. Epub 2022 Oct 31.
  PUBMED    36318920
REFERENCE   2  (bases 1 to 9645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.09.036"; journalName: "Cell"; date: 
            "2022-11-10- 10"; volume: "185"; issue: "23"; pages: "4376-4393.e18"
COMMENT     Sequence Label: pCDH-CMV-V5-RFP-MST2
FEATURES             Location/Qualifiers
     source          1..9645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2302..2343
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2368..3060
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(2498..2516)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2766..2785
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     RBS             3735..3743
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        4599..4810
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4823..5091
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5118..5717
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5118..5137)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5614..5634
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5724..6312
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5777..5797)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6195..6206)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6386..6619
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6691..6812
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6728..6747)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6782..6801
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6852..6987
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7020..7036)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7020..7036)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7033..7055)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7044..7060
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7068..7074,7075..7092,7093..7098))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7113..7134
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7251..7268)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7422..8010)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8181..8972,8973..9041))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8804..8823
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9042..9146)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9214..9232
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9270..9292)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9392..9411
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9605..9627
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9619..9636
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9620..9636
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gcgaattcat gggtaagcct atccctaacc ctctcctcgg tctcgattct
     2341 acgggcggtg ggtcgggtgg aggctcagtg agcaagggcg aggcagtgat caaggagttc
     2401 atgcggttca aggtgcacat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     2461 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     2521 ggccccctgc ccttctcctg ggacatcctg tcccctcagt tcatgtacgg ctccagggcc
     2581 ttcatcaagc accccgccga catccccgac tactataagc agtccttccc cgagggcttc
     2641 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc
     2701 tccctggagg acggcaccct gatctacaag gtgaagctcc gcggcaccaa cttccctcct
     2761 gacggccccg taatgcagaa gaagacaatg ggctgggaag cgtccaccga gcggttgtac
     2821 cccgaggacg gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa ggacggcggc
     2881 cgctacctgg cggacttcaa gaccacctac aaggccaaga agcccgtgca gatgcccggc
     2941 gcctacaacg tcgaccgcaa gttggacatc acctcccaca acgaggacta caccgtggtg
     3001 gaacagtacg aacgctccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     3061 ggtggaggct cggggggtgg ctcggagcag ccgccggcgc ctaagagtaa actaaaaaag
     3121 ctgagtgaag acagtttgac taagcagcct gaagaagttt ttgatgtatt agagaagctt
     3181 ggagaagggt cttatggaag tgtatttaaa gcaatacaca aggaatccgg tcaagttgtc
     3241 gcaattaaac aagtacctgt tgaatcagat cttcaggaaa taatcaaaga aatttccata
     3301 atgcagcaat gtgacagccc atatgttgta aagtactatg gcagttattt taagaataca
     3361 gacctctgga ttgttatgga gtactgtggc gctggctctg tctcagacat aattagatta
     3421 cgaaacaaga cattaataga agatgaaatt gcaaccattc ttaaatctac attgaaagga
     3481 ctagaatatt tgcactttat gagaaaaata cacagagata taaaagctgg aaatattctc
     3541 ctcaatacag aaggacatgc aaaattggca gattttggag tggctggtca gttaacagat
     3601 acaatggcaa aacgcaatac tgtaatagga actccatttt ggatggctcc tgaggtgatt
     3661 caagaaatag gctataactg tgtggccgac atctggtccc ttggcattac ttctatagaa
     3721 atggctgaag gaaaacctcc ttatgctgat atacatccaa tgagggctat ttttatgatt
     3781 cccacaaatc caccaccaac attcagaaag ccagaacttt ggtccgatga tttcaccgat
     3841 tttgttaaaa agtgtttggt gaagaatcct gagcagagag ctactgcaac acaactttta
     3901 cagcatcctt ttatcaagaa tgccaaacct gtatcaatat taagagacct gatcacagaa
     3961 gctatggaga tcaaagctaa aagacatgag gaacagcaac gagaattgga agaggaagaa
     4021 gaaaattcgg atgaagatga gctggattcc cacaccatgg tgaagactag tgtggagagt
     4081 gtgggcacca tgcgggccac aagcacgatg agtgaagggg cccagaccat gattgaacat
     4141 aatagcacga tgttggaatc cgacttgggg accatggtga taaacagtga ggatgaggaa
     4201 gaagaagatg gaactatgaa aagaaatgca acctcaccac aagtacaaag accatctttc
     4261 atggactact ttgataagca agacttcaag aataagagtc acgaaaactg taatcagaac
     4321 atgcatgagc ccttccctat gtccaaaaac gtttttcctg ataactggaa agttcctcaa
     4381 gatggagact ttgacttttt gaaaaatcta agtttagaag aactacagat gcggttaaaa
     4441 gcactggacc ccatgatgga acgggagata gaagaacttc gtcagagata cactgcgaaa
     4501 agacagccca ttctggatgc gatggatgca aagaaaagaa ggcagcaaaa cttttgagcg
     4561 gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     4621 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg
     4681 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     4741 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     4801 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac
     4861 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc
     4921 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc
     4981 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct
     5041 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg
     5101 accggcgcct acgctagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     5161 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     5221 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     5281 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     5341 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     5401 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     5461 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     5521 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     5581 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     5641 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     5701 gcaagcccgg tgcctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     5761 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     5821 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5881 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     5941 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6001 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6061 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     6121 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6181 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6241 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6301 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6361 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagataagat
     6421 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6481 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6541 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6601 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6661 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     6721 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6781 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     6841 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6901 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6961 agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt cgtaatcatg
     7021 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7081 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7141 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7201 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7261 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7321 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7381 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7441 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7501 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7561 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7621 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7681 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7741 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7801 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7861 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7921 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7981 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8041 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8101 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8161 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8221 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8281 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8341 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8401 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8461 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8521 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8581 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8641 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8701 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8761 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8821 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8881 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8941 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9001 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9061 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9121 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     9181 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     9241 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     9301 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     9361 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     9421 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     9481 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     9541 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     9601 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
