LOCUS       sequence-470272-       11562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express RFP tagged LATS1 gene in mammalian cells using the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11562)
  AUTHORS   Wang L, Choi K, Su T, Li B, Wu X, Zhang R, Driskill JH, Li H, Lei H,
            Guo P, Chen EH, Zheng Y, Pan D
  TITLE     Multiphase coalescence mediates Hippo pathway activation.
  JOURNAL   Cell. 2022 Nov 10;185(23):4376-4393.e18. doi: 
            10.1016/j.cell.2022.09.036. Epub 2022 Oct 31.
  PUBMED    36318920
REFERENCE   2  (bases 1 to 11562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.09.036"; journalName: "Cell"; date: 
            "2022-11-10- 10"; volume: "185"; issue: "23"; pages: "4376-4393.e18"
COMMENT     Sequence Label: pCDH-CMV-V5-RFP-LATS1
FEATURES             Location/Qualifiers
     source          1..11562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2302..2343
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2368..3060
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(2498..2516)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2766..2785
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        6516..6727
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6740..7008
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             7035..7634
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7035..7054)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7531..7551
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7641..8229
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7694..7714)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8112..8123)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8303..8536
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8608..8729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8645..8664)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8699..8718
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8769..8904
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8937..8953)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8937..8953)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8950..8972)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8961..8977
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8985..8991,8992..9009,9010..9015))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9030..9051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9168..9185)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9339..9927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(10098..10889,10890..10958))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10721..10740
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10959..11063)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11131..11149
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11187..11209)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11309..11328
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11522..11544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11536..11553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11537..11553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gcgaattcat gggtaagcct atccctaacc ctctcctcgg tctcgattct
     2341 acgggcggtg ggtcgggtgg aggctcagtg agcaagggcg aggcagtgat caaggagttc
     2401 atgcggttca aggtgcacat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     2461 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     2521 ggccccctgc ccttctcctg ggacatcctg tcccctcagt tcatgtacgg ctccagggcc
     2581 ttcatcaagc accccgccga catccccgac tactataagc agtccttccc cgagggcttc
     2641 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc
     2701 tccctggagg acggcaccct gatctacaag gtgaagctcc gcggcaccaa cttccctcct
     2761 gacggccccg taatgcagaa gaagacaatg ggctgggaag cgtccaccga gcggttgtac
     2821 cccgaggacg gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa ggacggcggc
     2881 cgctacctgg cggacttcaa gaccacctac aaggccaaga agcccgtgca gatgcccggc
     2941 gcctacaacg tcgaccgcaa gttggacatc acctcccaca acgaggacta caccgtggtg
     3001 gaacagtacg aacgctccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     3061 ggtggaggct cggggggtgg ctcgaagagg agtgaaaagc cagaaggata tagacaaatg
     3121 aggcctaaga cctttcctgc cagtaactat actgtcagta gccggcaaat gttacaagaa
     3181 attcgggaat cccttaggaa tttatctaaa ccatctgatg ctgctaaggc tgagcataac
     3241 atgagtaaaa tgtcaaccga agatcctcga caagtcagaa atccacccaa atttgggacg
     3301 catcataaag ccttgcagga aattcgaaac tctctgcttc catttgcaaa tgaaacaaat
     3361 tcttctcgga gtacttcaga agttaatcca caaatgcttc aagacttgca agctgctgga
     3421 tttgatgagg atatggttat acaagctctt cagaaaacta acaacagaag tatagaagca
     3481 gcaattgaat tcattagtaa aatgagttac caagatcctc gacgagagca gatggctgca
     3541 gcagctgcca gacctattaa tgccagcatg aaaccaggga atgtgcagca atcagttaac
     3601 cgcaaacaga gctggaaagg ttctaaagaa tccttagttc ctcagaggca tggcccgcca
     3661 ctaggagaaa gtgtggccta tcattctgag agtcccaact cacagacaga tgtaggaaga
     3721 cctttgtctg gatctggtat atcagcattt gttcaagctc accctagcaa cggacagaga
     3781 gtgaaccccc caccaccacc tcaagtaagg agtgttactc ctccaccacc tccaagaggc
     3841 cagactcccc ctccaagagg tacaactcca cctccccctt catgggaacc aaactctcaa
     3901 acaaagcgct attctggaaa catggaatac gtaatctccc gaatctctcc tgtcccacct
     3961 ggggcatggc aagagggcta tcctccacca cctctcaaca cttcccccat gaatcctcct
     4021 aatcaaggac agagaggcat tagttctgtt cctgttggca gacaaccaat catcatgcag
     4081 agttctagca aatttaactt tccatcaggg agacctggaa tgcagaatgg tactggacaa
     4141 actgatttca tgatacacca aaatgttgtc cctgctggca ctgtgaatcg gcagccacca
     4201 cctccatatc ctctgacagc agctaatgga caaagccctt ctgctttaca aacaggggga
     4261 tctgctgctc cttcgtcata tacaaatgga agtattcctc agtctatgat ggtgccaaac
     4321 agaaatagtc ataacatgga actatataac attagtgtac ctggactgca aacaaattgg
     4381 cctcagtcat cttctgctcc agcccagtca tccccgagca gtgggcatga aatccctaca
     4441 tggcaaccta acataccagt gaggtcaaat tcttttaata acccattagg aaatagagca
     4501 agtcactctg ctaattctca gccttctgct acaacagtca ctgcaattac accagctcct
     4561 attcaacagc ctgtgaaaag tatgcgtgta ttaaaaccag agctacagac tgctttagca
     4621 cctacacacc cttcttggat accacagcca attcaaactg ttcaacccag tccttttcct
     4681 gagggaaccg cttcaaatgt gactgtgatg ccacctgttg ctgaagctcc aaactatcaa
     4741 ggaccaccac caccctaccc aaaacatctg ctgcaccaaa acccatctgt tcctccatac
     4801 gagtcaatca gtaagcctag caaagaggat cagccaagct tgcccaagga agatgagagt
     4861 gaaaagagtt atgaaaatgt tgatagtggg gataaagaaa agaaacagat tacaacttca
     4921 cctattactg ttaggaaaaa caagaaagat gaagagcgaa gggaatctcg tattcaaagt
     4981 tattctcctc aagcatttaa attctttatg gagcaacatg tagaaaatgt actcaaatct
     5041 catcagcagc gtctacatcg taaaaaacaa ttagagaatg aaatgatgcg ggttggatta
     5101 tctcaagatg cccaggatca aatgagaaag atgctttgcc aaaaagaatc taattacatc
     5161 cgtcttaaaa gggctaaaat ggacaagtct atgtttgtga agataaagac actaggaata
     5221 ggagcatttg gtgaagtctg tctagcaaga aaagtagata ctaaggcttt gtatgcaaca
     5281 aaaactcttc gaaagaaaga tgttcttctt cgaaatcaag tcgctcatgt taaggctgag
     5341 agagatatcc tggctgaagc tgacaatgaa tgggtagttc gtctatatta ttcattccaa
     5401 gataaggaca atttatactt tgtaatggac tacattcctg ggggtgatat gatgagccta
     5461 ttaattagaa tgggcatctt tccagaaagt ctggcacgat tctacatagc agaacttacc
     5521 tgtgcagttg aaagtgttca taaaatgggt tttattcata gagatattaa acctgataat
     5581 attttgattg atcgtgatgg tcatattaaa ttgactgact ttggcctctg cactggcttc
     5641 agatggacac acgattctaa gtactatcag agtggtgacc atccacggca agatagcatg
     5701 gatttcagta atgaatgggg ggatccctca agctgtcgat gtggagacag actgaagcca
     5761 ttagagcgga gagctgcacg ccagcaccag cgatgtctag cacattcttt ggttgggact
     5821 cccaattata ttgcacctga agtgttgcta cgaacaggat acacacagtt gtgtgattgg
     5881 tggagtgttg gtgttattct ttttgaaatg ttggtgggac aacctccttt cttggcacaa
     5941 acaccattag aaacacaaat gaaggttatc aactggcaaa catctcttca cattccacca
     6001 caagctaaac tcagtcctga agcttctgat cttattatta aactttgccg aggacccgaa
     6061 gatcgcttag gcaagaatgg tgctgatgaa ataaaagctc atccattttt taaaacaatt
     6121 gacttctcca gtgacctgag acagcagtct gcttcataca ttcctaaaat cacacaccca
     6181 acagatacat caaattttga tcctgttgat cctgataaat tatggagtga tgataacgag
     6241 gaagaaaatg taaatgacac tctcaatgga tggtataaaa atggaaagca tcctgaacat
     6301 gcattctatg aatttacctt ccgaaggttt tttgatgaca atggctaccc atataattat
     6361 ccgaagccta ttgaatatga atacattaat tcacaaggct cagagcagca gtcggatgaa
     6421 gatgatcaaa acacaggctc agagattaaa aatcgcgatc tagtatatgt ttaagcggcc
     6481 gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     6541 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
     6601 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     6661 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     6721 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     6781 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     6841 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     6901 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     6961 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     7021 ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     7081 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     7141 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     7201 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     7261 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     7321 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     7381 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7441 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     7501 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     7561 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     7621 agcccggtgc ctgagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     7681 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7741 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7801 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7861 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7921 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7981 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     8041 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     8101 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     8161 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     8221 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     8281 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag ataagatctg
     8341 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8401 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8461 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8521 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     8581 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     8641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8701 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     8761 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8821 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8881 agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt aatcatgtca
     8941 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9001 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9061 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9121 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10021 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10081 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10141 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10201 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10261 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10321 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10381 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10441 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10501 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10561 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10621 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10681 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10741 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10801 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10861 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10921 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10981 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11041 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    11101 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    11161 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    11221 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    11281 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
    11341 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
    11401 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    11461 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    11521 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
