LOCUS       sequence-470275-       13141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of FL human VPS34 and human VPS15-ZZ in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Ohashi Y, Soler N, Garcia Ortegon M, Zhang L, Kirsten ML, Perisic O,
            Masson GR, Burke JE, Jakobi AJ, Apostolakis AA, Johnson CM, Ohashi 
            M, Ktistakis NT, Sachse C, Williams RL
  TITLE     Characterization of Atg38 and NRBF2, a fifth subunit of the 
            autophagic Vps34/PIK3C3 complex.
  JOURNAL   Autophagy. 2016 Nov;12(11):2129-2144. doi: 
            10.1080/15548627.2016.1226736. Epub 2016 Sep 14.
  PUBMED    27630019
REFERENCE   2  (bases 1 to 13141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2016.1226736"; journalName: "Autophagy"; date: 
            "2016-11"; volume: "12"; issue: "11"; pages: "2129-2144"
COMMENT     Sequence Label: pYO1025
FEATURES             Location/Qualifiers
     source          1..13141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..585
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     536..556
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    587..605
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    608..626
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     636..660
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(3458..3475)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3464..3688
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3745..4048
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          4328..5344
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5268..5290
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5352..5371
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      5418..5427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9519..9539
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             9561..9581
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             9663..9683
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             9699..9872
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             9873..10046
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     primer_bind     complement(10163..10182)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10228..10283
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10282..10301)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10644..10660)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10644..10660)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10657..10679)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10668..10684
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10692..10698,10699..10716,10717..10722))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10737..10758
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10817..11012
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10863..10998
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    11018..11152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11055..11074)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11109..11128
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11220..11237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11391..11979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11471..11490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12150..12941,12942..13010))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12773..12792
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13011..13115)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      481 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
      601 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      661 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      721 ctccggactc tagcgtttaa acttaagctt ggtaccatgg gggaagcaga gaagtttcac
      781 tacatctata gttgtgacct ggatatcaac gtccagctta agataggaag cttggaaggg
      841 aagagagaac aaaagagtta taaagctgtc ctggaagacc caatgttgaa gttctcagga
      901 ctatatcaag agacatgctc tgatctttat gttacttgtc aagtttttgc agaagggaag
      961 cctttggcct tgccagtgag aacatcctac aaagcattta gtacaagatg gaactggaat
     1021 gaatggctga aactaccagt aaaataccct gacctgccca ggaatgccca agtggccctc
     1081 accatctggg atgtgtatgg tcccggaaaa gcagtgcctg taggaggaac aacggtttcg
     1141 ctctttggaa aatacggcat gtttcgccaa gggatgcatg acttgaaagt ctggcctaat
     1201 gtagaagcag atggatcaga acccacaaaa actcctggca gaacaagtag cactctctca
     1261 gaagatcaga tgagccgtct tgccaagctc accaaagctc atcgacaagg acacatggtg
     1321 aaagtagatt ggctggatag attgacattt agagaaatag aaatgataaa tgagagtgaa
     1381 aaacgaagtt ctaatttcat gtacctgatg gttgaatttc gatgtgtcaa gtgtgatgat
     1441 aaggaatatg gtattgttta ttatgaaaag gacggtgatg aatcatctcc aattttaaca
     1501 agttttgaat tagtgaaagt tcctgacccc cagatgtcta tggagaattt agttgagagc
     1561 aaacaccaca agcttgcccg gagtttaaga agtggacctt ctgaccacga tctgaaaccc
     1621 aatgctgcca cgagagatca gttaaatatt attgtgagtt atccaccaac caagcaactt
     1681 acctatgaag aacaagatct tgtttggaag tttagatatt atcttacgaa tcaagaaaaa
     1741 gccttgacaa aattcttgaa atgtgttaat tgggatctac ctcaagaggc caaacaggcc
     1801 ttggaacttc tgggaaaatg gaagccgatg gatgtagagg actccttgga gctgttatcc
     1861 tctcattaca ccaacccaac tgtgaggcgt tatgctgttg cccggttgcg acaggccgat
     1921 gatgaggatt tgttgatgta cctattacaa ttggtccagg ctctcaaata tgaaaatttt
     1981 gatgatataa agaatggatt ggaacctacc aagaaggata gtcagagttc agtgtcagaa
     2041 aatgtgtcaa attctggaat aaattctgca gaaatagata gctcccaaat tataaccagc
     2101 ccccttcctt cagtctcttc acctcctcct gcatcaaaaa caaaagaagt tccagatggc
     2161 gaaaatctgg aacaagatct ctgtaccttc ttgatatcga gagcctgcaa aaactcaaca
     2221 ctggctaatt atttatactg gtatgtgata gtggaatgtg aagatcaaga tactcagcag
     2281 agagatccaa agacccatga gatgtacttg aacgtaatga gaagattcag ccaagcattg
     2341 ttgaagggtg ataagtctgt cagagttatg cgttctttgc tggctgcaca acagacattt
     2401 gtagatcggt tggtgcatct aatgaaggca gtacaacgcg aaagtggaaa tcgtaagaaa
     2461 aagaatgaga gactacaggc attgcttgga gataatgaaa agatgaattt gtcagatgtg
     2521 gaacttatcc cgttgccttt agaaccccaa gtgaaaatta gaggaataat tccggaaaca
     2581 gctacactgt ttaaaagtgc ccttatgcct gcacagttgt tttttaagac ggaagatgga
     2641 ggcaaatatc cagttatatt taagcatgga gatgatttac gtcaagatca acttattctt
     2701 caaatcattt cactcatgga caagctgtta cggaaagaaa atctggactt gaaattgaca
     2761 ccttataagg tgttagccac cagtacaaaa catggcttca tgcagtttat ccagtcagtt
     2821 cctgtggctg aagttcttga tacagaggga agcattcaga acttttttag aaaatatgca
     2881 ccaagtgaga atgggccaaa tgggattagt gctgaggtca tggacactta cgttaaaagc
     2941 tgtgctggat attgcgtgat cacctatata cttggagttg gagacaggca cctggataac
     3001 cttttgctaa caaaaacagg caaactcttc cacatagact ttggatatat tctgggtcgt
     3061 gatccaaagc ctcttcctcc accaatgaag ctgaataaag aaatggtaga aggaatgggg
     3121 ggcacacaga gtgagcagta ccaagagttc cgtaaacagt gttacacggc tttcctccac
     3181 ctgcgaaggt attctaatct gattttgaac ttgttttcct tgatggttga tgcaaacatt
     3241 ccagatattg cacttgaacc agataaaact gtgaaaaagg ttcaggataa attccgctta
     3301 gacctgtcgg atgaagaggc tgtgcattac atgcagagtc tgattgatga gagtgtccat
     3361 gctctttttg ctgcagtggt ggaacagatt cacaagtttg cccagtactg gcgcaaatga
     3421 gtcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     3481 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     3541 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     3601 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     3661 catgctggtg atgcggtggg ctctatggta ttaatagtaa tcaattacgg ggtcattagt
     3721 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     3781 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     3841 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     3901 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     3961 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     4021 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
     4081 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     4141 tgcagcgatg ggggcggggg gggggggggg gcgcgcgccr gggggggggg gggggggsgg
     4201 ggggsggggs ggggsgrggs ggagaggkgc ggcggcagcc aatcagagcg gcgcgctccg
     4261 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     4321 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
     4381 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     4441 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     4501 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     4561 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     4621 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     4681 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     4741 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     4801 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     4861 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     4921 cggggcgggg ccgcctcggg ccggggrggg ctcgggggrg gggcgcggcg gcccccggag
     4981 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     5041 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     5101 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     5161 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     5221 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     5281 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     5341 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgct
     5401 cgatcgacgg tatcgatgcc accatgggaa atcagcttgc tggcattgct ccctcccaga
     5461 tcctttctgt agagagttat ttttcagata ttcatgactt tgaatatgat aaaagcctgg
     5521 ggagtactcg gttttttaaa gttgctcgag ccaagcaccg agaaggcctg gtcgttgtga
     5581 aggtttttgc aattcaggat cccacattgc ctttaaccag ctataaacaa gagctggagg
     5641 aactgaaaat caggcttaat tctgcacaga attgtctacc tttccagaaa gcatcagaaa
     5701 aagcatctga gaaagcagct atgctcttta ggcagtatgt gcgagacaat ctctatgatc
     5761 gcatcagtac ccgtccattc ttgaataaca ttgagaagcg ctggattgct ttccagatcc
     5821 tgacagctgt ggaccaagca cacaaatctg gagttcgtca tggggacatc aagactgaga
     5881 atgtgatggt caccagttgg aattgggttc ttctaactga ttttgccagt tttaagccca
     5941 cttatcttcc agaagacaac ccggcagatt tcaattattt ctttgacaca tcacggagga
     6001 gaacttgcta tattgctcct gaacgttttg ttgatggtgg gatgtttgcc actgagttag
     6061 aatatatgag agatccttca actccgcttg tagacttaaa tagcaatcag agaacaagag
     6121 gagagttgaa gagagcaatg gacatctttt cagcaggttg tgtgatagct gagcttttta
     6181 cagaaggtgt accattattt gatctctctc aacttttggc ttatagaaat ggacattttt
     6241 tccctgaaca agtgctaaat aaaattgaag atcacagtat cagagaattg gtaactcaga
     6301 tgattcaccg tgagccagat aaacgtttag aggcagaaga ttacttaaaa cagcagcgtg
     6361 gcaatgcctt tcctgaaata ttttacactt ttcttcagcc ctacatggcc cagtttgcca
     6421 aggaaacgtt tctttctgca gatgagcgta ttctggttat acggaaggat ttgggcaaca
     6481 ttattcacaa tctctgtgga catgatctgc cagaaaaagc cgaaggagag cctaaggaaa
     6541 atgggctggt tatcttggta tctgttataa catcctgcct acagaccctt aaatactgtg
     6601 attccaaact agctgctttg gaactgattc ttcatttggc tccaagatta agtgttgaaa
     6661 tccttttgga tcgtattact ccatatcttt tgcatttcag caatgactct gttcctaggg
     6721 tgagggctga agccttgagg acgttgacca aagttcttgc tctcgtcaaa gaggttcctc
     6781 gtaatgatat caatatttat ccggaataca ttctgccagg catagcccac ttagcccaag
     6841 atgatgctac tatcgttaga ctagcctatg ctgaaaacat agctctgctg gcagaaacag
     6901 ctctgagatt cctggaatta gtacagttaa aaaatcttaa tatggaaaat gaccccaata
     6961 atgaagaaat agatgaggtt acacatccaa atggaaatta tgacacagag ctgcaagcct
     7021 tacatgaaat ggtccagcag aaagttgtta ctttgctaag tgaccctgaa aatattgtaa
     7081 aacaaacctt gatggaaaat ggaataacac ggctgtgtgt attctttgga cgtcagaaag
     7141 ccaacgatgt tttgttgtcc cacatgatta ctttcctaaa tgataagaat gattggcatc
     7201 tacgtggagc attttttgat agtatagttg gtgttgctgc ctatgttggc tggcaaagct
     7261 cctcaattct caagcctctg ctgcaacaag gtcttagtga tgctgaggaa tttgtcattg
     7321 tgaaagctct ttatgccctt acttgtatgt gccagttagg actgctacaa aaaccccatg
     7381 tttacgaatt tgccagtgat attgccccct tcctgtgtca tcccaattta tggatacgtt
     7441 atggtgccgt gggatttatc acagtggtag ctcgtcaaat aagtacagct gatgtctact
     7501 gtaaactgat gccttatctt gacccatata ttacccaacc aataatacag attgaaagaa
     7561 aacttgttct gctcagtgtt ttaaaggaac cagtaagtcg ttctatattt gattatgctt
     7621 tgaggtctaa agatattact agcttgttca gacatcttca catgcgtcag aagaaacgaa
     7681 atggttctct tcccgactgc cctccgccag aggatcctgc catagcacag cttctgaaga
     7741 agttgctctc acagggaatg acagaggaag aggaagacaa acttctggca ctgaaagact
     7801 tcatgatgaa atctaataaa gcaaaggcca atatagtgga ccagagccat cttcatgata
     7861 gtagtcagaa aggtgtaatt gacttggcag ctttaggcat aactgggaga caagttgatc
     7921 ttgttaaaac caaacaagaa ccagatgaca aacgggccag aaaacatgta aaacaagact
     7981 caaatgtaaa tgaagaatgg aaaagcatgt ttgggtcact ggacccacca aacatgccac
     8041 aggccctacc taaagggagt gatcaggagg tgattcagac tgggaaacct cctcgttccg
     8101 agtcctctgc tggcatttgt gtccctttgt caacttcttc acaggttcca gaagtgacaa
     8161 ctgtccaaaa taaaaaacca gtaataccgg ttttaagtag tacaatctta ccatccacct
     8221 atcagattcg aattacaact tgtaaaactg aacttcagca actcatccag caaaagcggg
     8281 agcagtgcaa tgctgagaga atagctaagc agatgatgga aaatgctgaa tgggagagta
     8341 aaccaccacc acctggatgg cgtcctaaag ggctgttagt tgcccatctt catgagcata
     8401 aatctgctgt gaatcgaatt agagtctctg atgaacactc actttttgca acatgttcaa
     8461 atgatggcac agtgaaaatc tggaacagtc aaaagatgga ggggaagacc accactacca
     8521 gatctattct tacatacagc cgaattggag gacgagtcaa gacgctcaca ttctgccaag
     8581 gctcccacta tttagccata gcatctgata atggtgctgt ccagcttctt ggaattgagg
     8641 cttctaagct gcccaagtct cctaaaatcc atcctctaca aagcagaatt ctagatcaga
     8701 aggaggacgg ttgtgttgtg gatatgcatc acttcaactc tggagcacag tctgttcttg
     8761 cctatgccac tgtgaatggc tctctggttg gctgggacct taggtcttca agcaatgcgt
     8821 ggactttaaa gcatgattta aagtcgggcc tcatcacttc ctttgctgtg gacatccacc
     8881 aatgctggct ctgcattggt acaagcagtg gcaccatggc ttgttgggac atgaggttcc
     8941 agttgccaat ttcaagtcac tgtcatcctt ccagggctcg aatcagacgc ctctcaatgc
     9001 accctctgta tcagtcctgg gtgattgcag ctgttcaggg caacaacgaa gtgtccatgt
     9061 gggacatgga gactggtgac agaagattta ctctctgggc cagcagtgca ccaccacttt
     9121 ctgaattaca gccttctcct catagcgtcc atggtatcta ctgtagtcct gcagatggaa
     9181 atcctatcct actaacagct ggctcagata tgaaaataag gttttgggac ttggcttacc
     9241 cagaaaggtc ctatgttgtt gcaggaagta ctagttcccc atctgtgtcc tactacagga
     9301 aaataattga aggcactgaa gttgtccagg aaattcagaa taagcagaaa gtaggaccaa
     9361 gtgatgacac ccctcgaagg ggcccagagt ccctgcccgt gggacatcat gacatcatca
     9421 ctgatgtcgc cacattccag accacacagg gcttcatcgt aactgcttct agagatggga
     9481 ttgtgaaggt gtggaaatct agaccaacta ctgcctctga aaatttgtac tttcaaggcg
     9541 agttgaagac cactgcttct gaaaacttgt acttccaagg tgagttactc gagggtgaat
     9601 tgaagactgc tgctttggct caacacgacg aagctagatc cggagcaggg gcgggtgcta
     9661 gcgagaacct gtacttccag ggcgcccacg atgaagccgt agacaacaaa ttcaacaaag
     9721 aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa gaacaacgaa
     9781 acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt ttagcagaag
     9841 ctaaaaagct aaatgatgct caggcgccga aagtagacaa caaattcaac aaagaacaac
     9901 aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa cgaaacgcct
     9961 tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca gaagctaaaa
    10021 agctaaatga tgctcaggcg ccgaaagtag acgcgaattc gtgagctcgc gaactcgagg
    10081 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
    10141 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
    10201 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
    10261 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
    10321 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
    10381 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
    10441 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
    10501 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
    10561 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
    10621 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    10681 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    10741 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    10801 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
    10861 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    10921 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    10981 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
    11041 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    11101 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
    11161 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    11221 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    11281 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    11341 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    11401 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    11461 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    11521 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    11581 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    11641 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    11701 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    11761 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    11821 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    11881 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    11941 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    12001 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    12061 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    12121 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    12181 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    12241 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    12301 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    12361 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    12421 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    12481 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    12541 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    12601 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    12661 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    12721 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    12781 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    12841 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    12901 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    12961 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    13021 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    13081 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    13141 g
//
