LOCUS       sequence-470278-       11531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express YFP tagged KIBRA gene in mammalian cells using the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11531)
  AUTHORS   Wang L, Choi K, Su T, Li B, Wu X, Zhang R, Driskill JH, Li H, Lei H,
            Guo P, Chen EH, Zheng Y, Pan D
  TITLE     Multiphase coalescence mediates Hippo pathway activation.
  JOURNAL   Cell. 2022 Nov 10;185(23):4376-4393.e18. doi: 
            10.1016/j.cell.2022.09.036. Epub 2022 Oct 31.
  PUBMED    36318920
REFERENCE   2  (bases 1 to 11531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.09.036"; journalName: "Cell"; date: 
            "2022-11-10- 10"; volume: "185"; issue: "23"; pages: "4376-4393.e18"
COMMENT     Sequence Label: pCDH-CMV-FLAG-YFP-KIBRA
FEATURES             Location/Qualifiers
     source          1..11531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2302..2325
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(2350..2352,2353..3063)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2392..2413)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     3000..3021
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6485..6696
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6709..6977
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             7004..7603
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7004..7023)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7500..7520
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7610..8198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7663..7683)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8081..8092)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8272..8505
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8577..8698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8614..8633)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8668..8687
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8738..8873
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8906..8922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8906..8922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8919..8941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8930..8946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8954..8960,8961..8978,8979..8984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8999..9020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9137..9154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9308..9896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(10067..10858,10859..10927))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10690..10709
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10928..11032)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11100..11118
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11156..11178)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11278..11297
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11491..11513
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11505..11522
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11506..11522
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gcgaattcat ggattacaag gatgatgatg ataagggcgg tgggtcgggt
     2341 ggaggctcag tgagcaaggg cgaggagctg ttcaccggcg tggtgcccat cctggtcgag
     2401 ctggacggcg acgtgaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgacgcc
     2461 accaacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
     2521 cccaccctcg tgaccaccct gggctacggc gtgcagtgct tcgcccgcta ccccgaccac
     2581 atgaagcagc acgacttctt caagtccgcc atgcccgagg gctacgtcca ggagcgcacc
     2641 atcttcttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac
     2701 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2761 ggccacaagc tggagtacaa cttcaacagc cacaacgtct acatcaccgc cgacaagcag
     2821 aagaacggca tcaaggccaa cttcaagatc cgccacaacg tggaggacgg cggcgtgcag
     2881 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2941 aaccactacc tgagctacca gtccaagctg agcaaggacc ccaacgagaa gcgcgaccac
     3001 atggtcctgc tggagttcgt gaccgccgcc ggcatcaccc acggcatgga cgagctgtac
     3061 aagggtggag gctcgggggg tggctcgccc cggccggagc tgcccctgcc ggagggctgg
     3121 gaggaggcgc gcgacttcga cggcaaggtc tactacatag accacacgaa ccgcaccacc
     3181 agctggatcg acccgcggga caggtacacc aaaccgctca cctttgctga ctgcattagt
     3241 gatgagttgc cgctaggatg ggaagaggca tatgacccac aggttggaga ttacttcata
     3301 gaccacaaca ccaaaaccac tcagattgag gatcctcgag tacaatggcg gcgggagcag
     3361 gaacatatgc tgaaggatta cctggtggtg gcccaggagg ctctgagtgc acaaaaggag
     3421 atctaccagg tgaagcagca gcgcctggag cttgcacagc aggagtacca gcaactgcat
     3481 gccgtctggg agcataagct gggctcccag gtcagcttgg tctctggttc atcatccagc
     3541 tccaagtatg accctgagat cctgaaagct gaaattgcca ctgcaaaatc ccgggtcaac
     3601 aagctgaaga gagagatggt tcacctccag cacgagctgc agttcaaaga gcgtggcttt
     3661 cagaccctga agaaaatcga taagaaaatg tctgatgctc agggcagcta caaactggat
     3721 gaagctcagg ctgtcttgag agaaacaaaa gccatcaaaa aggctattac ctgtggggaa
     3781 aaggaaaagc aagatctcat taagagcctt gccatgttga aggacggctt ccgcactgac
     3841 agggggtctc actcagacct gtggtccagc agcagctctc tggagagttc gagtttcccg
     3901 ctaccgaaac agtacctgga tgtgagctcc cagacagaca tctcgggaag cttcggcatc
     3961 aacagcaaca atcagttggc agagaaggtc agattgcgcc ttcgatatga agaggctaag
     4021 agaaggatcg ccaacctgaa gatccagctg gccaagcttg acagtgaggc ctggcctggg
     4081 gtgctggact cagagaggga ccggctgatc cttatcaacg agaaggagga gctgctgaag
     4141 gagatgcgct tcatcagccc cctcaagtgg acccaggggg aggtggagca gctggagatg
     4201 gcccggaagc ggctggaaaa ggacctgcag gcagcccggg acacccagag caaggcgctg
     4261 acggagaggt taaagttaaa cagtaagagg aaccagcttg tgagagaact ggaggaagcc
     4321 acccggcagg tggcaactct gcactcccag ctgaaaagtc tctcaagcag catgcagtcc
     4381 ctgtcctcag gcagcagccc cggatccctc acgtccagcc ggggctccct ggttgcatcc
     4441 agcctggact cctccacttc agccagcttc actgacctct actatgaccc ctttgagcag
     4501 ctggactcag agctgcagag caaggtggag ttcctgctcc tggagggggc caccggcttc
     4561 cggccctcag gctgcatcac caccatccac gaggatgagg tggccaagac ccagaaggca
     4621 gagggaggtg gccgcctgca ggctctgcgt tccctgtctg gcaccccaaa gtccatgacc
     4681 tccctatccc cacgttcctc tctctcctcc ccctccccac cctgttcccc tctcatggct
     4741 gaccccctcc tggctggtga tgccttcctc aactccttgg agtttgaaga cccggagctg
     4801 agtgccactc tttgtgaact gagccttggt aacagcgccc aggaaagata ccggctggag
     4861 gaaccaggaa cggagggcaa gcagctgggc caagctgtga atacggccca ggggtgtggc
     4921 ctgaaagtgg cctgtgtctc agccgccgta tcggacgagt cagtggctgg agacagtggt
     4981 gtgtacgagg cttccgtgca gagactgggt gcttcagaag ctgctgcatt tgacagtgac
     5041 gaatcggaag cagtgggtgc gacccgaatt cagattgccc tgaagtatga tgagaagaat
     5101 aagcaatttg caatattaat catccagctg agtaaccttt ctgctctgtt gcagcaacaa
     5161 gaccagaaag tgaatatccg cgtggctgtc cttccttgct ctgaaagcac aacctgcctg
     5221 ttccggaccc ggcctctgga cgcctcagac actctagtgt tcaatgaggt gttctgggta
     5281 tccatgtcct atccagccct tcaccagaag accttaagag tcgatgtctg taccaccgac
     5341 aggagccatc tggaagagtg cctgggaggc gcccagatca gcctggcgga ggtctgccgg
     5401 tctggggaga ggtcgactcg ctggtacaac cttctcagct acaaatactt gaagaagcag
     5461 agcagggagc tcaagccagt gggagttatg gcccctgcct cagggcctgc cagcacggac
     5521 gctgtgtctg ctctgttgga acagacagca gtggagctgg agaagaggca ggagggcagg
     5581 agcagcacac agacactgga agacagctgg aggtatgagg agaccagtga gaatgaggca
     5641 gtagccgagg aagaggagga ggaggtggag gaggaggagg gagaagagga tgttttcacc
     5701 gagaaagcct cacctgatat ggatgggtac ccagcattaa aggtggacaa agagaccaac
     5761 acggagaccc cggccccatc ccccacagtg gtgcgaccta aggaccggag agtgggcacc
     5821 ccgtcccagg ggccatttct tcgagggagc accatcatcc gctctaagac cttctcccca
     5881 ggaccccaga gccagtacgt gtgccggctg aatcggagtg atagtgacag ctccactctg
     5941 tccaaaaagc caccttttgt tcgaaactcc ctggagcgac gcagcgtccg gatgaagcgg
     6001 ccgtccccac ccccacagcc ttcctcggtc aagtcgctgc gctccgagcg tctgatccgt
     6061 acctcgctgg acctggagtt agacctgcag gcgacaagaa cctggcacag ccaactgacc
     6121 caggagatct cggtgctgaa ggagctcaag gagcagctgg aacaagccaa gagccacggg
     6181 gagaaggagc tgccacagtg gttgcgtgag gacgagcgtt tccgcctgct gctgaggatg
     6241 ctggagaagc ggcagatgga ccgagcggag cacaagggtg agcttcagac agacaagatg
     6301 atgagggcag ctgccaagga tgtgcacagg ctccgaggcc agagctgtaa ggaaccccca
     6361 gaagttcagt ctttcaggga gaagatggca tttttcaccc gcctcggatg aatatcccag
     6421 ctctctctgc agatgacgtc taagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     6481 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     6541 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     6601 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     6661 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     6721 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     6781 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     6841 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     6901 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     6961 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg agtacaagcc
     7021 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     7081 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     7141 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     7201 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     7261 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     7321 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     7381 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     7441 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     7501 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     7561 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca atcaacctct
     7621 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7681 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7741 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7801 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7861 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7921 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7981 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     8041 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     8101 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     8161 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga
     8221 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8281 taattcactc ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag
     8341 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8401 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8461 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     8521 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     8581 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8641 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8701 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8761 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8821 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt
     8881 ttgcagagac caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
     8941 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9001 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9061 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9121 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9181 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9241 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9301 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9361 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9421 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9481 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9541 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9601 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9661 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9721 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9781 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9841 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9901 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9961 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10021 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10081 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10141 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10201 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10261 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10321 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10381 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10441 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10501 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10561 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10621 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10681 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10741 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10801 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10861 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10921 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10981 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11041 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    11101 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    11161 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    11221 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    11281 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    11341 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    11401 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    11461 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    11521 gtgccaagct g
//
