LOCUS       sequence-470279-        8675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express TAZ-CAMTA1 fusion gene in mammalian cells using the 
            doxycycline-inducible TRE promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Driskill JH, Zheng Y, Wu BK, Wang L, Cai J, Rakheja D, Dellinger M, 
            Pan D
  TITLE     WWTR1(TAZ)-CAMTA1 reprograms endothelial cells to drive epithelioid 
            hemangioendothelioma.
  JOURNAL   Genes Dev. 2021 Apr 1;35(7-8):495-511. doi: 10.1101/gad.348221.120. 
            Epub 2021 Mar 25.
  PUBMED    33766984
REFERENCE   2  (bases 1 to 8675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2021-04-1"; volume: "35(7-8):495-511. doi"; pages: " 
            10.1101/gad.348221.120. Epub 2021 Mar 25"
COMMENT     Sequence Label: pTRE-TAZ-CAMTA1
FEATURES             Location/Qualifiers
     source          1..8675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    19..37
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    61..79
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    103..121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    145..163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        196..234
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     231..255
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      574..583
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             583..606
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             613..636
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          5463..6035
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(6167..6186)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6232..6287
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6286..6305)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6685..6702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6856..7444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6936..6955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7615..8406,8407..8475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8238..8257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8476..8580)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8648..8666
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcactcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
       61 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      121 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct
      181 cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      241 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      301 cgggaccgat ccagcctccg cggccccgaa ttcgagctcg gtaccgtcga caagtgacca
      361 gctacagtcg gaaaccatca gcaagcaggt atgtactctc cagggcgggc ctggcttccc
      421 cagtcaagac tccagggatt tgagggacgc tgtgggctca actcttacat gtaccttttg
      481 ctagcctcaa ccctgactat cttccaggtc attgttccaa cctcggatcc tctagtcagc
      541 tgacgcgtgc tagcgcggcc gcatcgataa gctgccacca tggattataa agatgatgat
      601 gataaagggg cagactacaa ggacgacgac gacaagaatc cggcctcggc gccccctccg
      661 ctcccgccgc ctgggcagca agtgatccac gtcacgcagg acctagacac agacctcgaa
      721 gccctcttca actctgtcat gaatccgaag cctagctcgt ggcggaagaa gatcctgccg
      781 gagtctttct ttaaggagcc tgattcgggc tcgcactcgc gccagtccag caccgactcg
      841 tcgggcggcc acccggggcc tcgactggct gggggtgccc agcatgtccg ctcgcactcg
      901 tcgcccgcgt ccctgcagct gggcaccggc gcgggtgctg cgggtagccc cgcgcagcag
      961 cacgcgcacc tccgccagca gtcctacgac gtgaccgacg agctgccact gcccccgggc
     1021 tgggagatga ccttcacggc cactggccag aggtacttcc tcaatcacat agaaaaaatc
     1081 accacatggc aagaccctag gaaggcgatg aatcagcctc tgaatcatat gaacctccac
     1141 cctgccgtca gttccacacc agtgcctcag aggtccatgg cagtatccca gccaaatctc
     1201 ggagctggcg gcagcgtgca tcacaagtgt aacagcgcca aacaccgcat catctcgccc
     1261 aaggtggagc cacggacagg ggggtacggg agccactcgg aggtgcagca caatgacgtg
     1321 tcggagggca agcacgagca cagccacagc aagggctcca gccgtgagaa gaggaacggc
     1381 aaggtggcca agcccgtgct cctgcaccag agcagcaccg aggtctcctc caccaaccag
     1441 gtggaagtcc ccgacaccac ccagagctcc cctgtgtcca tcagcagcgg gctcaacagc
     1501 gacccggaca tggtggacag cccggtggtc acaggtgtgt ccggtatggc ggtggcctct
     1561 gtgatgggga gcttgtccca gagcgccacg gtgttcatgt cagaggtcac caatgaggcc
     1621 gtgtacacca tgtcccccac cgctggcccc aaccaccacc tcctctcacc tgacgcctct
     1681 cagggcctcg tcctggccgt gagctctgat ggccacaagt tcgcctttcc caccacgggc
     1741 agctcagaga gcctgtccat gctgcccacc aacgtgtccg aagagctggt cctctccacc
     1801 accctcgacg gtggccggaa gattccagaa accaccatga actttgaccc cgactgtttc
     1861 cttaataacc caaagcaggg ccagacgtac gggggtggag gcctgaaagc cgagatggtc
     1921 agctccaaca tccggcactc gccacccggg gagcggagct tcagctttac caccgtcctc
     1981 accaaggaga tcaagaccga ggacacctcc ttcgagcagc agatggccaa agaagcgtac
     2041 tcctcctccg cggcggctgt ggcagccagc tccctcaccc tgaccgccgg ctccagcctc
     2101 ctgccgtcgg gcggcggcct gagtcccagc accaccctgg agcagatgga cttcagcgcc
     2161 atcgactcca acaaggacta cacgtccagc ttcagccaga cgggccacag cccccacatc
     2221 caccagaccc cctccccgag cttcttcctg caggacgcca gcaaacccct ccccgtcgag
     2281 cagaacaccc acagcagcct gagtgactct gggggcacct tcgtgatgcc cacggtgaaa
     2341 acggaggcct cgtcccaaac cagctcctgc agcggtcacg tggagacgcg gatcgagtcc
     2401 acttcctccc tccacctcat gcagttccag gccaacttcc aggccatgac ggcagaaggg
     2461 gaggtcacca tggagacctc gcaggcggcg gaagggagcg aggtcctgct caagtctggg
     2521 gagctgcagg cttgcagctc tgagcactac ctgcagccgg agaccaacgg ggtaatccga
     2581 agcgccggcg gcgtccccat cctcccgggc aacgtggtgc agggactcta ccccgtggcc
     2641 cagcccagcc tcggcaacgc ctccaacatg gagctcagcc tggaccactt tgacatctcc
     2701 ttcagcaacc agttctccga cctgatcaac gacttcatct ccgtggaggg gggcagcagc
     2761 accatctatg ggcaccagct ggtgtcgggg gacagcacgg cgctctcaca gtcagaggac
     2821 ggggcgcggg cccccttcac ccaggcagag atgtgcctcc cctgctgtag cccccagcag
     2881 ggtagcctgc agctgagcag ctcggagggc ggggccagca ccatggccta catgcacgtc
     2941 gccgaggtgg tctcggccgc ctcggcccag ggcaccctag gcatgctgca gcagagcgga
     3001 cgggtgttca tggtgaccga ctactcccca gagtggtctt acccagaggg aggagtgaag
     3061 gtcctcatca caggcccgtg gcaagaagcc agcaataact acagctgcct gtttgaccag
     3121 atctcagtgc ctgcatccct gattcagcct ggggtgctgc gctgctactg cccagcccat
     3181 gacactggtc ttgtgaccct acaagttgcc ttcaacaacc agatcatctc caactcggtg
     3241 gtgtttgagt acaaagcccg ggctctgccc acgctccctt cctcccagca cgactggctg
     3301 tcgttggacg ataaccagtt caggatgtcc atcctggaac gactggagca gatggagagg
     3361 aggatggccg agatgacggg gtcccagcag cacaaacagg cgagcggagg cggcagcagt
     3421 ggaggcggca gcgggagcgg gaatggaggg agccaggcac agtgtgcttc tgggactggg
     3481 gccttgggga gctgctttga gagccgtgtg gtcgtggtat gcgagaagat gatgagccga
     3541 gcctgctggg cgaagtccaa gcacttgatc cactcaaaga ctttccgcgg aatgacccta
     3601 ctccacctgg ccgctgccca gggctatgcc accctaatcc agaccctcat caaatggcgt
     3661 acaaagcacg cggatagcat tgacctggaa ctggaagttg accccttgaa tgtggaccac
     3721 ttctcctgta ctcctctgat gtgggcgtgt gccctagggc acttggaagc tgccgtcgtg
     3781 ctgtacaagt gggaccgtcg ggccatctcg attcccgact ctctaggaag gctgcctttg
     3841 ggaattgcca ggtcacgggg tcatgtgaaa ttagcagagt gtctggagca cctgcagaga
     3901 gatgagcagg ctcagctggg acagaacccc agaatccact gtcctgcaag cgaagagccc
     3961 agcacagaga gctggatggc ccagtggcac agcgaagcca tcagctctcc agaaataccc
     4021 aagggagtca ctgttattgc aagcaccaac ccagagctga gaagacctcg ttctgaaccc
     4081 tctaattact acagcagtga gagccacaaa gattatccgg ctcccaaaaa gcataaattg
     4141 aaccctgagt acttccagac aaggcaggag aagctgcttc ccactgcact gagtctggaa
     4201 gagccaaata tcaggaagca aagccctagt tctaagcagt ctgtccccga gacactcagc
     4261 cccagtgaag gagtgaggga cttcagccgg gaactctccc ctcccactcc agagactgca
     4321 gcatttcaag cctctggatc tcagcctgta ggaaagtgga attccaaaga tctttacatt
     4381 ggtgtgtcta cagtacaggt gactggaaat ccgaagggga ccagtgtagg aaaggaggca
     4441 gcaccttcac aggtgcgtcc acgggaacca atgagtgtcc tgatgatggc taacagagag
     4501 gtggtgaata cagagctggg gtcctaccgt gatagtgcag aaaatgaaga atgcggccag
     4561 cccatggatg acatacaggt gaacatgatg accttggcag aacacattat tgaagccaca
     4621 cctgaccgaa tcaagcagga gaattttgtg cccatggagt cctcaggatt ggaaagaaca
     4681 gaccctgcca ccattagcag tacaatgagc tggctggcca gttatctagc ggatgctgac
     4741 tgccttccca gtgctgccca gatccgaagt gcatataacg agcctctaac cccttcttct
     4801 aataccagct tgagccctgt tggctctccc gtcagtgaaa tcgctttcga gaaacctaac
     4861 cttccctccg ccgcggattg gtcagaattc ctgagtgcat ctaccagtga gaaggtagag
     4921 aatgagtttg ctcagctcac tctgtctgat catgaacaga gagaactcta tgaggctgcc
     4981 aggcttgtcc agacagcttt ccggaaatac aagggccgac ccttgcggga acagcaagaa
     5041 gtagctgctg ctgttattca gcgttgttac agaaaatata aacagtacgc actttataaa
     5101 aagatgacac aggctgccat ccttatccag agcaaattcc gaagttacta tgaacaaaaa
     5161 aaattccagc agagccgacg ggctgctgtg ctcatccaaa agtactaccg aagttataag
     5221 aaatgtggca aaagacggca ggctcgccgg acggctgtga ttgtacaaca gaaactcagg
     5281 agcagtttgc taaccaaaaa gcaggatcaa gctgctcgaa aaataatgag gtttcttcgc
     5341 cgctgtcgcc acagccccct ggtggaccat aggctgtaca aaaggagtga aagaattgaa
     5401 aaaggccaag gaacttgagt cgacagcttg tcgacgatat ctctagagct gagaacttca
     5461 gggtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa ttcatgttat
     5521 atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc ttgtatcacc
     5581 atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa ccattgtctc
     5641 ctcttatttt cttttcattt tctgtaactt tttcgttaaa ctttagcttg catttgtaac
     5701 gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc taatcacttt
     5761 tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag agaacaattg
     5821 ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg cgtggaaata
     5881 ttcttattgg tagaaacaac tacaccctgg tcatcatcct gcctttctct ttatggttac
     5941 aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg
     6001 ctaaccatgt tcatgccttc ttctctttcc tacagctcct gggcaacgtg ctggttgttg
     6061 tgctgtctca tcattttggc aaagaattca ctcctcaggt gcaggctgcc tatcagaagg
     6121 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
     6181 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     6241 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     6301 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     6361 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     6421 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     6481 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     6541 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     6601 tcttatggag atccctcgac tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6661 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6721 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6781 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6841 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6901 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6961 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7021 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7081 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7141 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7201 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7261 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7321 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7381 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7441 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7501 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7561 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7621 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7681 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7741 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7801 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7861 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7921 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7981 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8041 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8101 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8161 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8221 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8281 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8341 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8401 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8461 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8521 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8581 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     8641 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
