LOCUS       sequence-470280-       10226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express TAZ-CAMTA1 fusion gene in mammalian cells using the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Driskill JH, Zheng Y, Wu BK, Wang L, Cai J, Rakheja D, Dellinger M, 
            Pan D
  TITLE     WWTR1(TAZ)-CAMTA1 reprograms endothelial cells to drive epithelioid 
            hemangioendothelioma.
  JOURNAL   Genes Dev. 2021 Apr 1;35(7-8):495-511. doi: 10.1101/gad.348221.120. 
            Epub 2021 Mar 25.
  PUBMED    33766984
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2021-04-1"; volume: "35(7-8):495-511. doi"; pages: " 
            10.1101/gad.348221.120. Epub 2021 Mar 25"
COMMENT     Sequence Label: pCDNA-TAZ-CAMTA1
FEATURES             Location/Qualifiers
     source          1..10226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      935..944
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             944..967
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             974..997
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5817..5834)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5823..6047
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6093..6521
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6180..6199)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6390..6411
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6530..6550)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6535..6864
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6715..6850
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6777..6796
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6931..7725
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6985..7004)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7595..7614
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7899..8020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7936..7955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7990..8009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8069..8085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8069..8085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8082..8104)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8093..8109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8117..8123,8124..8141,8142..8147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8162..8183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8300..8317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8471..9059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8551..8570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9230..10021,10022..10090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9853..9872
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10091..10195)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggattata aagatgatga
      961 tgataaaggg gcagactaca aggacgacga cgacaagaat ccggcctcgg cgccccctcc
     1021 gctcccgccg cctgggcagc aagtgatcca cgtcacgcag gacctagaca cagacctcga
     1081 agccctcttc aactctgtca tgaatccgaa gcctagctcg tggcggaaga agatcctgcc
     1141 ggagtctttc tttaaggagc ctgattcggg ctcgcactcg cgccagtcca gcaccgactc
     1201 gtcgggcggc cacccggggc ctcgactggc tgggggtgcc cagcatgtcc gctcgcactc
     1261 gtcgcccgcg tccctgcagc tgggcaccgg cgcgggtgct gcgggtagcc ccgcgcagca
     1321 gcacgcgcac ctccgccagc agtcctacga cgtgaccgac gagctgccac tgcccccggg
     1381 ctgggagatg accttcacgg ccactggcca gaggtacttc ctcaatcaca tagaaaaaat
     1441 caccacatgg caagacccta ggaaggcgat gaatcagcct ctgaatcata tgaacctcca
     1501 ccctgccgtc agttccacac cagtgcctca gaggtccatg gcagtatccc agccaaatct
     1561 cggagctggc ggcagcgtgc atcacaagtg taacagcgcc aaacaccgca tcatctcgcc
     1621 caaggtggag ccacggacag gggggtacgg gagccactcg gaggtgcagc acaatgacgt
     1681 gtcggagggc aagcacgagc acagccacag caagggctcc agccgtgaga agaggaacgg
     1741 caaggtggcc aagcccgtgc tcctgcacca gagcagcacc gaggtctcct ccaccaacca
     1801 ggtggaagtc cccgacacca cccagagctc ccctgtgtcc atcagcagcg ggctcaacag
     1861 cgacccggac atggtggaca gcccggtggt cacaggtgtg tccggtatgg cggtggcctc
     1921 tgtgatgggg agcttgtccc agagcgccac ggtgttcatg tcagaggtca ccaatgaggc
     1981 cgtgtacacc atgtccccca ccgctggccc caaccaccac ctcctctcac ctgacgcctc
     2041 tcagggcctc gtcctggccg tgagctctga tggccacaag ttcgcctttc ccaccacggg
     2101 cagctcagag agcctgtcca tgctgcccac caacgtgtcc gaagagctgg tcctctccac
     2161 caccctcgac ggtggccgga agattccaga aaccaccatg aactttgacc ccgactgttt
     2221 ccttaataac ccaaagcagg gccagacgta cgggggtgga ggcctgaaag ccgagatggt
     2281 cagctccaac atccggcact cgccacccgg ggagcggagc ttcagcttta ccaccgtcct
     2341 caccaaggag atcaagaccg aggacacctc cttcgagcag cagatggcca aagaagcgta
     2401 ctcctcctcc gcggcggctg tggcagccag ctccctcacc ctgaccgccg gctccagcct
     2461 cctgccgtcg ggcggcggcc tgagtcccag caccaccctg gagcagatgg acttcagcgc
     2521 catcgactcc aacaaggact acacgtccag cttcagccag acgggccaca gcccccacat
     2581 ccaccagacc ccctccccga gcttcttcct gcaggacgcc agcaaacccc tccccgtcga
     2641 gcagaacacc cacagcagcc tgagtgactc tgggggcacc ttcgtgatgc ccacggtgaa
     2701 aacggaggcc tcgtcccaaa ccagctcctg cagcggtcac gtggagacgc ggatcgagtc
     2761 cacttcctcc ctccacctca tgcagttcca ggccaacttc caggccatga cggcagaagg
     2821 ggaggtcacc atggagacct cgcaggcggc ggaagggagc gaggtcctgc tcaagtctgg
     2881 ggagctgcag gcttgcagct ctgagcacta cctgcagccg gagaccaacg gggtaatccg
     2941 aagcgccggc ggcgtcccca tcctcccggg caacgtggtg cagggactct accccgtggc
     3001 ccagcccagc ctcggcaacg cctccaacat ggagctcagc ctggaccact ttgacatctc
     3061 cttcagcaac cagttctccg acctgatcaa cgacttcatc tccgtggagg ggggcagcag
     3121 caccatctat gggcaccagc tggtgtcggg ggacagcacg gcgctctcac agtcagagga
     3181 cggggcgcgg gcccccttca cccaggcaga gatgtgcctc ccctgctgta gcccccagca
     3241 gggtagcctg cagctgagca gctcggaggg cggggccagc accatggcct acatgcacgt
     3301 cgccgaggtg gtctcggccg cctcggccca gggcacccta ggcatgctgc agcagagcgg
     3361 acgggtgttc atggtgaccg actactcccc agagtggtct tacccagagg gaggagtgaa
     3421 ggtcctcatc acaggcccgt ggcaagaagc cagcaataac tacagctgcc tgtttgacca
     3481 gatctcagtg cctgcatccc tgattcagcc tggggtgctg cgctgctact gcccagccca
     3541 tgacactggt cttgtgaccc tacaagttgc cttcaacaac cagatcatct ccaactcggt
     3601 ggtgtttgag tacaaagccc gggctctgcc cacgctccct tcctcccagc acgactggct
     3661 gtcgttggac gataaccagt tcaggatgtc catcctggaa cgactggagc agatggagag
     3721 gaggatggcc gagatgacgg ggtcccagca gcacaaacag gcgagcggag gcggcagcag
     3781 tggaggcggc agcgggagcg ggaatggagg gagccaggca cagtgtgctt ctgggactgg
     3841 ggccttgggg agctgctttg agagccgtgt ggtcgtggta tgcgagaaga tgatgagccg
     3901 agcctgctgg gcgaagtcca agcacttgat ccactcaaag actttccgcg gaatgaccct
     3961 actccacctg gccgctgccc agggctatgc caccctaatc cagaccctca tcaaatggcg
     4021 tacaaagcac gcggatagca ttgacctgga actggaagtt gaccccttga atgtggacca
     4081 cttctcctgt actcctctga tgtgggcgtg tgccctaggg cacttggaag ctgccgtcgt
     4141 gctgtacaag tgggaccgtc gggccatctc gattcccgac tctctaggaa ggctgccttt
     4201 gggaattgcc aggtcacggg gtcatgtgaa attagcagag tgtctggagc acctgcagag
     4261 agatgagcag gctcagctgg gacagaaccc cagaatccac tgtcctgcaa gcgaagagcc
     4321 cagcacagag agctggatgg cccagtggca cagcgaagcc atcagctctc cagaaatacc
     4381 caagggagtc actgttattg caagcaccaa cccagagctg agaagacctc gttctgaacc
     4441 ctctaattac tacagcagtg agagccacaa agattatccg gctcccaaaa agcataaatt
     4501 gaaccctgag tacttccaga caaggcagga gaagctgctt cccactgcac tgagtctgga
     4561 agagccaaat atcaggaagc aaagccctag ttctaagcag tctgtccccg agacactcag
     4621 ccccagtgaa ggagtgaggg acttcagccg ggaactctcc cctcccactc cagagactgc
     4681 agcatttcaa gcctctggat ctcagcctgt aggaaagtgg aattccaaag atctttacat
     4741 tggtgtgtct acagtacagg tgactggaaa tccgaagggg accagtgtag gaaaggaggc
     4801 agcaccttca caggtgcgtc cacgggaacc aatgagtgtc ctgatgatgg ctaacagaga
     4861 ggtggtgaat acagagctgg ggtcctaccg tgatagtgca gaaaatgaag aatgcggcca
     4921 gcccatggat gacatacagg tgaacatgat gaccttggca gaacacatta ttgaagccac
     4981 acctgaccga atcaagcagg agaattttgt gcccatggag tcctcaggat tggaaagaac
     5041 agaccctgcc accattagca gtacaatgag ctggctggcc agttatctag cggatgctga
     5101 ctgccttccc agtgctgccc agatccgaag tgcatataac gagcctctaa ccccttcttc
     5161 taataccagc ttgagccctg ttggctctcc cgtcagtgaa atcgctttcg agaaacctaa
     5221 ccttccctcc gccgcggatt ggtcagaatt cctgagtgca tctaccagtg agaaggtaga
     5281 gaatgagttt gctcagctca ctctgtctga tcatgaacag agagaactct atgaggctgc
     5341 caggcttgtc cagacagctt tccggaaata caagggccga cccttgcggg aacagcaaga
     5401 agtagctgct gctgttattc agcgttgtta cagaaaatat aaacagtacg cactttataa
     5461 aaagatgaca caggctgcca tccttatcca gagcaaattc cgaagttact atgaacaaaa
     5521 aaaattccag cagagccgac gggctgctgt gctcatccaa aagtactacc gaagttataa
     5581 gaaatgtggc aaaagacggc aggctcgccg gacggctgtg attgtacaac agaaactcag
     5641 gagcagtttg ctaaccaaaa agcaggatca agctgctcga aaaataatga ggtttcttcg
     5701 ccgctgtcgc cacagccccc tggtggacca taggctgtac aaaaggagtg aaagaattga
     5761 aaaaggccaa ggaacttgag tcgagtctag agggcccgtt taaacccgct gatcagcctc
     5821 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5881 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5941 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6001 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6061 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     6121 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6181 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6241 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6301 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6361 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6421 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6481 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     6541 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6601 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6661 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6721 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6781 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     6841 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     6901 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     6961 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     7021 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     7081 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     7141 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     7201 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     7261 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     7321 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     7381 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     7441 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     7501 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7561 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7621 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7681 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     7741 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     7801 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7861 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7921 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7981 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     8041 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8101 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8161 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8221 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8281 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8341 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8401 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8461 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8521 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8581 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8641 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8701 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8761 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8821 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8881 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8941 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9001 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9061 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9121 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9181 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9241 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9301 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9361 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9421 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9481 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9541 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9601 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9661 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9721 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9781 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9841 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9901 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9961 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10021 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10081 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10141 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10201 ttccccgaaa agtgccacct gacgtc
//
