LOCUS       sequence-470298-       11688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CFP tagged PTPN14 gene in mammalian cells using the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11688)
  AUTHORS   Wang L, Choi K, Su T, Li B, Wu X, Zhang R, Driskill JH, Li H, Lei H,
            Guo P, Chen EH, Zheng Y, Pan D
  TITLE     Multiphase coalescence mediates Hippo pathway activation.
  JOURNAL   Cell. 2022 Nov 10;185(23):4376-4393.e18. doi: 
            10.1016/j.cell.2022.09.036. Epub 2022 Oct 31.
  PUBMED    36318920
REFERENCE   2  (bases 1 to 11688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.09.036"; journalName: "Cell"; date: 
            "2022-11-10- 10"; volume: "185"; issue: "23"; pages: "4376-4393.e18"
COMMENT     Sequence Label: pCDH-CMV-PTPN14-CFP
FEATURES             Location/Qualifiers
     source          1..11688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(5884..5886,5887..6600)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5926..5947)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6187..6206)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6534..6555
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6642..6853
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6866..7134
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             7161..7760
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7161..7180)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7657..7677
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7767..8355
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7820..7840)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8238..8249)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8429..8662
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8734..8855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8771..8790)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8825..8844
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8895..9030
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9063..9079)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9063..9079)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9076..9098)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9087..9103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9111..9117,9118..9135,9136..9141))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9156..9177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9294..9311)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9465..10053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(10224..11015,11016..11084))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10847..10866
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11085..11189)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11257..11275
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11313..11335)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11435..11454
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11648..11670
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11662..11679
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11663..11679
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gcgaattcat gccttttggt ctgaagctcc gccggacacg gcgctacaac
     2341 gtcctgagca agaactgctt tgtcacacgg attcgcctgc tggacagcaa tgttatcgag
     2401 tgcacgctgt cggtggaaag cacagggcaa gaatgcctgg aggctgtggc ccagaggctg
     2461 gagctgcgag agacgcacta ctttggcctt tggtttctca gcaagagcca gcaagcacga
     2521 tgggtggagc tggagaaacc tctgaagaaa catctggaca aattcgctaa tgagcctttg
     2581 cttttctttg gagtcatgtt ctatgtgcca aatgtgtcat ggcttcagca agaggccaca
     2641 agatatcagt attacctgca agtcaaaaaa gatgtgcttg aagggcgatt acgatgtaca
     2701 ttggaccagg tgattcggct agccggccta gctgtgcaag ctgattttgg agactataat
     2761 cagtttgatt ctcaagattt cctcagagag tatgtgctat ttcctatgga tttggccctg
     2821 gaagaggctg ttctggagga gctgacccag aaggtagccc aagaacacaa agcccacagt
     2881 ggaatcctgc cagcagaagc tgaactgatg tacatcaatg aagttgaacg tttggatgga
     2941 tttggacagg aaatcttccc tgtaaaggac aatcatggaa actgtgtaca ccttggcatt
     3001 ttctttatgg ggattttcgt gaggaacaga attggaagac aagcggtaat atacaggtgg
     3061 aatgacatgg ggaatatcac tcataacaag tcgaccattc tagtggagct catcaacaaa
     3121 gaagagactg ccctctttca cacggatgat atcgaaaatg ccaagtatat ttctcggttg
     3181 tttgccacac gacacaagtt ttacaaacaa aacaaaatct gcactgaaca gtcaaattct
     3241 ccacccccca tcagacgcca gcccacctgg agccggtcct ctctgcccag gcagcagccg
     3301 tacatcctgc ctcccgttca cgtccagtgt ggtgagcact actcggaaac gcacacctcg
     3361 caagacagca tttttcatgg gaatgaagaa gccttgtatt gcaactctca caacagcctg
     3421 gacttaaatt atttaaatgg cactgtcacc aatggcagcg tgtgtagcgt tcacagcgtc
     3481 aactccctca actgctcgca aagtttcatc caggcctccc ctgtatcctc caacctcagt
     3541 atccctggga gtgacatcat gcgggccgac tacatcccga gccaccggca cagcgcgatc
     3601 atcgtgccct cgtacaggcc aacccccgat tatgagacag tcatgcgcca gatgaagagg
     3661 gggatcctgc atacagacag ccagagccag tctctgagaa acctcaacat tatcaacacc
     3721 catgcctaca accagccaga ggatctggtg tacagccaac cggagatgcg ggagaggcac
     3781 ccctacactg tcccttatgg gccacagggg gtctacagca acaaacttgt cagtccatct
     3841 gaccagagga acccaaagaa taatgtggta ccaagcaagc cgggggcaag cgccatctcg
     3901 cacacggtga gcaccccaga gctggccaac atgcagctgc agggcagcca taactacagc
     3961 acggcccaca tgcttaagaa ctatctcttc aggccaccgc ccccctaccc acggccacga
     4021 ccggccacca gcaccccaga cctggccagc caccgccaca agtacgtcag cggcagcagc
     4081 ccggacctgg tgacccggaa ggtgcagctc tcggtgaaga ccttccaaga ggacagctct
     4141 ccggtggttc atcagtctct ccaggaggtg agcgagcccc tcacggccac caagcaccac
     4201 ggcactgtga acaagcgcca cagcctggag gtgatgaaca gcatggtgcg gggcatggag
     4261 gccatgacgc tcaagtcgct ccacctcccc atggctcgcc gcaacacgct ccgggagcag
     4321 ggaccgcccg aggaggggtc aggcagccac gaggtccccc agctccctca gtatcaccac
     4381 aagaagacct tctctgatgc cactatgcta atccacagca gcgagagtga ggaggaggag
     4441 gaggaggctc cagaatcggt gccccagatc cccatgctcc gggagaagat ggagtacagt
     4501 gcccagctgc aggcggccct ggcccgcatc cccaacaagc ccccgcctga gtaccccggt
     4561 ccaaggaaga gtgtgagcaa tggggctctg aggcaggacc aagccagcct tcctcccgcc
     4621 atggccagag ccagggtgct gaggcatggg ccagccaagg ccatcagcat gtctcggact
     4681 gacccgccgg ctgtcaacgg ggcctctctc ggcccatcca tctcggaacc cgacctgact
     4741 agtgtgaagg agcgggtcaa aaaagagcct gtgaaggaga gacctgtgtc tgaaatgttt
     4801 tccctggaag acagcattat agagagagag atgatgatca ggaatctaga gaagcagaag
     4861 atggcaggcc tggaggcaca gaagaggccg ctgatgttgg cagcattgaa tgggctctcg
     4921 gtggctcgag tctcagggcg ggaagagaat cgagttgatg ccacccgggt tcccatggac
     4981 gagaggttca gaaccctgaa gaagaaacta gaagagggaa tggtgttcac agaatatgag
     5041 caaattccaa agaaaaaggc gaatggcatt ttcagcacag cagctctgcc agaaaacgcc
     5101 gagcgcagcc gaatccgtga agttgtcccc tatgaggaga atcgagtaga gctgatacca
     5161 accaaagaaa ataacacagg atacattaat gcctcccaca tcaaggtggt ggttggcggg
     5221 gcagaatggc actacatagc cacccagggg cccctgccac acacgtgcca cgacttctgg
     5281 cagatggtgt gggagcaggg agtgaatgtg attgccatgg tcactgcaga ggaggagggt
     5341 ggacgaacca aaagccaccg atactggccc aaactaggtt caaagcacag ctcagccacc
     5401 tatggcaagt tcaaggtcac cacgaagttt cgaacggatt ctgtttgcta tgcaaccacg
     5461 ggcttgaagg tcaagcacct tttgtctggg caagaaagga cggtgtggca tttacaatat
     5521 actgactggc cagatcacgg ctgtccagag gatgtccaag gatttttatc ctacttggag
     5581 gagatccagt cggtccgtcg ccataccaac agcatgctgg aaggcaccaa gaaccggcac
     5641 ccgcccatcg tggtccactg tagtgctggg gtgggaagga ccggcgtgct cattctttct
     5701 gagctgatga tctactgctt ggaacataac gaaaaggtgg aagtgcccat gatgctgagg
     5761 ctcctcaggg agcagaggat gttcatgatc cagactatcg ctcagtacaa gtttgtctac
     5821 caagtcctca tccagttcct ccaaaactcc agactcattg gtggaggctc ggggggtggc
     5881 tcggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     5941 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     6001 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     6061 ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     6121 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     6181 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     6241 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     6301 aagctggagt acaactactt tagcgacaac gtctatatca ccgccgacaa gcagaagaac
     6361 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
     6421 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6481 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     6541 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     6601 gcggccgcga aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc
     6661 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg
     6721 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     6781 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     6841 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc
     6901 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc
     6961 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg
     7021 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct
     7081 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct
     7141 gtgaccggcg cctacgctag atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     7201 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     7261 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     7321 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     7381 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     7441 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     7501 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     7561 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     7621 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     7681 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     7741 cccgcaagcc cggtgcctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     7801 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7861 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7921 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7981 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     8041 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     8101 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     8161 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     8221 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     8281 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     8341 gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     8401 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa
     8461 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8521 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8581 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8641 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     8701 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     8761 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8821 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     8881 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8941 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     9001 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa attcgtaatc
     9061 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9121 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9181 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9241 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9301 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9361 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9421 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9481 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9541 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9601 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9661 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9721 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9781 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9841 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9901 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9961 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10021 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10081 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10141 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10201 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10261 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10321 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10381 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10441 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10501 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10561 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10621 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10681 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10741 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10801 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10861 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10921 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10981 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    11041 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11101 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11161 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    11221 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    11281 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
    11341 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
    11401 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
    11461 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
    11521 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    11581 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
    11641 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
