LOCUS       sequence-470303-        9912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CFP tagged NF2 gene in mammalian cells using the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Wang L, Choi K, Su T, Li B, Wu X, Zhang R, Driskill JH, Li H, Lei H,
            Guo P, Chen EH, Zheng Y, Pan D
  TITLE     Multiphase coalescence mediates Hippo pathway activation.
  JOURNAL   Cell. 2022 Nov 10;185(23):4376-4393.e18. doi: 
            10.1016/j.cell.2022.09.036. Epub 2022 Oct 31.
  PUBMED    36318920
REFERENCE   2  (bases 1 to 9912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.09.036"; journalName: "Cell"; date: 
            "2022-11-10- 10"; volume: "185"; issue: "23"; pages: "4376-4393.e18"
COMMENT     Sequence Label: pCDH-CMV-NF2-CFP
FEATURES             Location/Qualifiers
     source          1..9912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(4108..4110,4111..4824)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4150..4171)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4411..4430)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4758..4779
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4866..5077
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5090..5358
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5385..5984
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5385..5404)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5881..5901
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5991..6579
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6044..6064)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6462..6473)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6653..6886
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6958..7079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6995..7014)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7049..7068
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7119..7254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7287..7303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7287..7303)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7300..7322)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7311..7327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7335..7341,7342..7359,7360..7365))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7380..7401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7518..7535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7689..8277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8448..9239,9240..9308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9071..9090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9309..9413)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9481..9499
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9537..9559)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9659..9678
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9872..9894
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9886..9903
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9887..9903
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gcgaattcat ggccggggcc atcgcttccc gcatgagctt cagctctctc
     2341 aagaggaagc aacccaagac gttcaccgtg aggatcgtca ccatggacgc cgagatggag
     2401 ttcaattgcg agatgaagtg gaaagggaag gacctctttg atttggtgtg ccggactctg
     2461 gggctccgag aaacctggtt ctttggactg cagtacacaa tcaaggacac agtggcctgg
     2521 ctcaaaatag acaagaaggt actggatcat gatgtttcaa aggaagaacc agtcaccttt
     2581 cacttcttgg ccaaatttta tcctgagaat gctgaagagg agctggttca ggagatcaca
     2641 caacatttat tcttcttaca ggtaaagaag cagattttag atgaaaagat ctactgccct
     2701 cctgaggctt ctgtgctcct ggcttcttac gccgtccagg ccaagtatgg tgactacgac
     2761 cccagtgttc acaagcgggg atttttggcc caagaggaat tgcttccaaa aagggtaata
     2821 aatctgtatc agatgactcc ggaaatgtgg gaggagagaa ttactgcttg gtacgcagag
     2881 caccgaggcc gagccaggga tgaagctgaa atggaatatc tgaagatagc tcaggacctg
     2941 gagatgtacg gtgtgaacta ctttgcaatc cggaataaaa agggcacaga gctgctgctt
     3001 ggagtggatg ccctggggct tcacatttat gaccctgaga acagactgac ccccaagatc
     3061 tccttcccgt ggaatgaaat ccgaaacatc tcgtacagtg acaaggagtt tactattaaa
     3121 ccactggata agaaaattga tgtcttcaag tttaactcct caaagcttcg tgttaataag
     3181 ctgattctcc agctatgtat cgggaaccat gatctattta tgaggagaag gaaagccgat
     3241 tctttggaag ttcagcagat gaaagcccag gccagggagg agaaggctag aaagcagatg
     3301 gagcggcagc gcctcgctcg agagaagcag atgagggagg aggctgaacg cacgagggat
     3361 gagttggaga ggaggctgct gcagatgaaa gaagaagcaa caatggccaa cgaagcactg
     3421 atgcggtctg aggagacagc tgacctgttg gctgaaaagg cccagatcac cgaggaggag
     3481 gcaaaacttc tggcccagaa ggccgcagag gctgagcagg aaatgcagcg catcaaggcc
     3541 acagcgattc gcacggagga ggagaagcgc ctgatggagc agaaggtgct ggaagccgag
     3601 gtgctggcac tgaagatggc tgaggagtca gagaggaggg ccaaagaggc agatcagctg
     3661 aagcaggacc tgcaggaagc acgcgaggcg gagcgaagag ccaagcagaa gctcctggag
     3721 attgccacca agcccacgta cccgcccatg aacccaattc cagcaccgtt gcctcctgac
     3781 ataccaagct tcaacctcat tggtgacagc ctgtctttcg acttcaaaga tactgacatg
     3841 aagcggcttt ccatggagat agagaaagaa aaagtggaat acatggaaaa gagcaagcat
     3901 ctgcaggagc agctcaatga actcaagaca gaaatcgagg ccttgaaact gaaagagagg
     3961 gagacagctc tggatattct gcacaatgag aactccgaca ggggtggcag cagcaagcac
     4021 aataccatta aaaagctcac cttgcagagc gccaagtccc gagtggcctt ctttgaagag
     4081 ctcggtggag gctcgggggg tggctcggtg agcaagggcg aggagctgtt caccggggtg
     4141 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     4201 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     4261 aagctgcccg tgccctggcc caccctcgtg accaccctgt cctggggcgt gcagtgcttc
     4321 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4381 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4441 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4501 gaggacggca acatcctggg gcacaagctg gagtacaact actttagcga caacgtctat
     4561 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     4621 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     4681 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
     4741 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4801 ggcatggacg agctgtacaa gtaagcggcc gcgaaggatc tgcgatcgct ccggtgcccg
     4861 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     4921 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     4981 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     5041 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
     5101 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
     5161 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
     5221 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
     5281 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
     5341 tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagatgacc gagtacaagc
     5401 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     5461 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     5521 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     5581 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     5641 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     5701 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     5761 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     5821 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     5881 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     5941 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagtcgac aatcaacctc
     6001 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6061 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6121 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6181 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6241 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6301 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6361 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     6421 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6481 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6541 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg
     6601 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     6661 ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc tctctggtta
     6721 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6781 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6841 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     6901 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     6961 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7021 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7081 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7141 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7201 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
     7261 tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     7321 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7381 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7441 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7501 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7561 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7621 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7681 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7741 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7801 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7861 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7921 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7981 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8041 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8101 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8161 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8221 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8281 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8341 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8401 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8461 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8521 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8581 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8641 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8701 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8761 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8821 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8881 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8941 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9001 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9061 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9121 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9181 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9241 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9301 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9361 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9421 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     9481 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     9541 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     9601 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     9661 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     9721 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     9781 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     9841 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     9901 agtgccaagc tg
//
