LOCUS       sequence-470305-       10533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express RFP tagged SLMAP gene in mammalian cells using the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   Wang L, Choi K, Su T, Li B, Wu X, Zhang R, Driskill JH, Li H, Lei H,
            Guo P, Chen EH, Zheng Y, Pan D
  TITLE     Multiphase coalescence mediates Hippo pathway activation.
  JOURNAL   Cell. 2022 Nov 10;185(23):4376-4393.e18. doi: 
            10.1016/j.cell.2022.09.036. Epub 2022 Oct 31.
  PUBMED    36318920
REFERENCE   2  (bases 1 to 10533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.09.036"; journalName: "Cell"; date: 
            "2022-11-10- 10"; volume: "185"; issue: "23"; pages: "4376-4393.e18"
COMMENT     Sequence Label: pCDH-CMV-V5-RFP-SLMAP (isoform r)
FEATURES             Location/Qualifiers
     source          1..10533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2302..2343
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2368..3060
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(2498..2516)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2766..2785
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        5487..5698
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5711..5979
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6006..6605
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6006..6025)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6502..6522
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6612..7200
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6665..6685)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7083..7094)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7274..7507
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7579..7700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7616..7635)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7670..7689
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7740..7875
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7908..7924)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7908..7924)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7921..7943)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7932..7948
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7956..7962,7963..7980,7981..7986))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8001..8022
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8139..8156)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8310..8898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9069..9860,9861..9929))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9692..9711
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9930..10034)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10102..10120
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10158..10180)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10280..10299
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10493..10515
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10507..10524
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10508..10524
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gcgaattcat gggtaagcct atccctaacc ctctcctcgg tctcgattct
     2341 acgggcggtg ggtcgggtgg aggctcagtg agcaagggcg aggcagtgat caaggagttc
     2401 atgcggttca aggtgcacat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     2461 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     2521 ggccccctgc ccttctcctg ggacatcctg tcccctcagt tcatgtacgg ctccagggcc
     2581 ttcatcaagc accccgccga catccccgac tactataagc agtccttccc cgagggcttc
     2641 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc
     2701 tccctggagg acggcaccct gatctacaag gtgaagctcc gcggcaccaa cttccctcct
     2761 gacggccccg taatgcagaa gaagacaatg ggctgggaag cgtccaccga gcggttgtac
     2821 cccgaggacg gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa ggacggcggc
     2881 cgctacctgg cggacttcaa gaccacctac aaggccaaga agcccgtgca gatgcccggc
     2941 gcctacaacg tcgaccgcaa gttggacatc acctcccaca acgaggacta caccgtggtg
     3001 gaacagtacg aacgctccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     3061 ggtggaggct cggggggtgg ctcgccgtca gccttggcca tcttcacttg ccgcccgaac
     3121 tcgcacccgt ttcaggagcg tcatgtctac ctggacgagc ccatcaaaat cggccgctca
     3181 gtggcccgct gtcgaccagc gcagaataat gccacttttg attgcaaagt gctatcaagg
     3241 aaccacgctc tcgtctggtt tgatcacaag acgggcaagt tttatcttca agacactaaa
     3301 agtagtaatg gtacttttat aaatagccag agattgagtc gaggctctga agaaagtcca
     3361 ccatgtgaaa ttctttccgg tgacattatc cagtttggag tagacgtgac agagaataca
     3421 cggaaagtta cccatgggtg tattgtttcc acaataaaac tttttctacc agatggtatg
     3481 gaagcccggc tccgctcaga tgtcatccat gcaccattac caagtcctgt tgacaaagtt
     3541 gctgctaaca ctccaagtat gtactctcag gaactattcc agctttctca gtatctacag
     3601 gaggccttac atcgggaaca aatgttggaa cagaagttag ccacgcttca gcggctacta
     3661 gccatcaccc aagaggcttc agataccagt tggcaggctt taatagatga agatagactc
     3721 ttatcacggt tagaagttat gggaaaccaa ttacaggcat gctccaaaaa tcaaacagaa
     3781 gatagtttac gaaaggaact tatagcatta caagaggata aacataacta tgagacaaca
     3841 gccaaagagt ccctgaggcg ggttcttcag gagaaaattg aagtggttag aaaactttca
     3901 gaagttgagc gaagtctgag taatactgaa gatgaatgta cccatctgaa agaaatgaat
     3961 gaaaggactc aggaagaatt aagagaatta gccaacaaat ataatggagc agttaatgag
     4021 attaaagatt tatctgataa attaaaggta gcagagggaa aacaagagga aatccaacag
     4081 aagggacagg ctgagaaaaa agaattacaa cataaaatag atgaaatgga agaaaaagaa
     4141 caggagctcc aggcaaaaat agaagctttg caagctgata atgatttcac caatgaaagg
     4201 ctaacagctt tacaagagaa gctgatcgtc gaagggcatc taaccaaagc ggtagaagaa
     4261 acaaagcttt caaaagaaaa tcagacaaga gcaaaagaat ctgatttttc agatactctg
     4321 agtccaagca aggaaaaaag cagtgacgac actacagacg cccaaatgga tgagcaagac
     4381 ctaaatgagc ctcttgccaa agtgtccctt ttaaaagatg acttgcaggg tgcacagtca
     4441 gaaattgagg caaagcaaga aatacagcat cttcgaaagg aattgatcga agcccaggag
     4501 ctagctagaa caagtaaaca aaaatgcttt gaacttcaag ctcttttgga agaagaaaga
     4561 aaagcctatc gaaatcaagt tgaggaatcc actaaacaaa tacaggttct tcaagcccaa
     4621 ttgcagaggt tacacatcga tactgagaat ctccgggagg agaaggacag tgaaatcaca
     4681 agtactagag atgaattgct tagtgcccga gatgaaattt tgctccttca tcaagcagca
     4741 gcaaaggttg cctctgagcg ggacactgac attgcttctt tacaagaaga gcttaagaag
     4801 gtgagagctg agcttgagcg gtggcggaaa gcagcgtctg aatatgagaa agaaatcaca
     4861 agtctgcaaa acagttttca gcttagatgt caacagtgtg aggaccagca gagagaagaa
     4921 gcaacaaggt tgcaaggtga actagagaag ttgagaaagg aatggaatgc attggaaacc
     4981 gaatgccatt ctctaaaaag ggaaaatgtt ttgctatcat cagaactgca acggcaagaa
     5041 aaagaattgc acaattctca gaagcagagt ttagagctta ccagtgatct cagcatcctt
     5101 caaatgtcta ggaaagaact tgagaatcaa gtgggatcct tgaaagaaca gcatcttcgg
     5161 gattcagctg atttaaaaac tcttctcagt aaggcagaaa accaagcaaa ggatgtgcag
     5221 aaagagtatg aaaagacaca gactgtactc tcagaactga agttgaagtt tgaaatgact
     5281 gagcaggaaa agcagtcaat cacagatgag ctcaaacagt gtaaaaacaa cctgaagctg
     5341 ctccgagaga aaggaaataa tccttccata ttacaacccg tcccagccgt attcatcggc
     5401 ctattcctgg ctttcctgtt ttggtgtttc ggtccattgt ggtaggcggc cgcgaaggat
     5461 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     5521 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     5581 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     5641 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     5701 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     5761 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     5821 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     5881 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     5941 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     6001 gctagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     6061 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     6121 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     6181 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     6241 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     6301 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     6361 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     6421 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     6481 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     6541 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     6601 cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6661 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6721 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6781 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6841 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6901 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6961 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7021 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7081 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7141 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7201 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7261 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct gctttttgct
     7321 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7381 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7441 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7501 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7561 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7621 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7681 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7741 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7801 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7861 gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc atagctgttt
     7921 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7981 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8041 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8101 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8161 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8221 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8281 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8341 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8401 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8461 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8521 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8581 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8641 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8701 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8761 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8821 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8881 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8941 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9001 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9061 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9121 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9181 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9241 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9301 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9361 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9421 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9481 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9541 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9601 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9661 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9721 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9781 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9841 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9901 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9961 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10021 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    10081 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    10141 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    10201 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    10261 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    10321 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
    10381 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    10441 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    10501 cgacgttgta aaacgacggc cagtgccaag ctg
//
