LOCUS       sequence-470307-       10539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CFP tagged SLMAP gene in mammalian cells using the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10539)
  AUTHORS   Wang L, Choi K, Su T, Li B, Wu X, Zhang R, Driskill JH, Li H, Lei H,
            Guo P, Chen EH, Zheng Y, Pan D
  TITLE     Multiphase coalescence mediates Hippo pathway activation.
  JOURNAL   Cell. 2022 Nov 10;185(23):4376-4393.e18. doi: 
            10.1016/j.cell.2022.09.036. Epub 2022 Oct 31.
  PUBMED    36318920
REFERENCE   2  (bases 1 to 10539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.09.036"; journalName: "Cell"; date: 
            "2022-11-10- 10"; volume: "185"; issue: "23"; pages: "4376-4393.e18"
COMMENT     Sequence Label: pCDH-CMV-HA-CFP-SLMAP (isoform r)
FEATURES             Location/Qualifiers
     source          1..10539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2302..2328
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(2353..2355,2356..3066)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2395..2416)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2656..2675)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3003..3024
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5493..5704
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5717..5985
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6012..6611
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6012..6031)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6508..6528
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6618..7206
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6671..6691)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7089..7100)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7280..7513
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7585..7706
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7622..7641)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7676..7695
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7746..7881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7914..7930)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7914..7930)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7927..7949)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7938..7954
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7962..7968,7969..7986,7987..7992))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8007..8028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8145..8162)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8316..8904)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9075..9866,9867..9935))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9698..9717
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9936..10040)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10108..10126
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10164..10186)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10286..10305
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10499..10521
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10513..10530
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10514..10530
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gcgaattcat gtacccgtac gatgtgccgg attacgccgg cggtgggtcg
     2341 ggtggaggct cagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2401 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2461 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2521 tggcccaccc tcgtgaccac cctgtcctgg ggcgtgcagt gcttcgcccg ctaccccgac
     2581 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2641 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2701 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2761 ctggggcaca agctggagta caactacttt agcgacaacg tctatatcac cgccgacaag
     2821 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     2881 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2941 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     3001 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3061 tacaagggtg gaggctcggg gggtggctcg ccgtcagcct tggccatctt cacttgccgc
     3121 ccgaactcgc acccgtttca ggagcgtcat gtctacctgg acgagcccat caaaatcggc
     3181 cgctcagtgg cccgctgtcg accagcgcag aataatgcca cttttgattg caaagtgcta
     3241 tcaaggaacc acgctctcgt ctggtttgat cacaagacgg gcaagtttta tcttcaagac
     3301 actaaaagta gtaatggtac ttttataaat agccagagat tgagtcgagg ctctgaagaa
     3361 agtccaccat gtgaaattct ttccggtgac attatccagt ttggagtaga cgtgacagag
     3421 aatacacgga aagttaccca tgggtgtatt gtttccacaa taaaactttt tctaccagat
     3481 ggtatggaag cccggctccg ctcagatgtc atccatgcac cattaccaag tcctgttgac
     3541 aaagttgctg ctaacactcc aagtatgtac tctcaggaac tattccagct ttctcagtat
     3601 ctacaggagg ccttacatcg ggaacaaatg ttggaacaga agttagccac gcttcagcgg
     3661 ctactagcca tcacccaaga ggcttcagat accagttggc aggctttaat agatgaagat
     3721 agactcttat cacggttaga agttatggga aaccaattac aggcatgctc caaaaatcaa
     3781 acagaagata gtttacgaaa ggaacttata gcattacaag aggataaaca taactatgag
     3841 acaacagcca aagagtccct gaggcgggtt cttcaggaga aaattgaagt ggttagaaaa
     3901 ctttcagaag ttgagcgaag tctgagtaat actgaagatg aatgtaccca tctgaaagaa
     3961 atgaatgaaa ggactcagga agaattaaga gaattagcca acaaatataa tggagcagtt
     4021 aatgagatta aagatttatc tgataaatta aaggtagcag agggaaaaca agaggaaatc
     4081 caacagaagg gacaggctga gaaaaaagaa ttacaacata aaatagatga aatggaagaa
     4141 aaagaacagg agctccaggc aaaaatagaa gctttgcaag ctgataatga tttcaccaat
     4201 gaaaggctaa cagctttaca agagaagctg atcgtcgaag ggcatctaac caaagcggta
     4261 gaagaaacaa agctttcaaa agaaaatcag acaagagcaa aagaatctga tttttcagat
     4321 actctgagtc caagcaagga aaaaagcagt gacgacacta cagacgccca aatggatgag
     4381 caagacctaa atgagcctct tgccaaagtg tcccttttaa aagatgactt gcagggtgca
     4441 cagtcagaaa ttgaggcaaa gcaagaaata cagcatcttc gaaaggaatt gatcgaagcc
     4501 caggagctag ctagaacaag taaacaaaaa tgctttgaac ttcaagctct tttggaagaa
     4561 gaaagaaaag cctatcgaaa tcaagttgag gaatccacta aacaaataca ggttcttcaa
     4621 gcccaattgc agaggttaca catcgatact gagaatctcc gggaggagaa ggacagtgaa
     4681 atcacaagta ctagagatga attgcttagt gcccgagatg aaattttgct ccttcatcaa
     4741 gcagcagcaa aggttgcctc tgagcgggac actgacattg cttctttaca agaagagctt
     4801 aagaaggtga gagctgagct tgagcggtgg cggaaagcag cgtctgaata tgagaaagaa
     4861 atcacaagtc tgcaaaacag ttttcagctt agatgtcaac agtgtgagga ccagcagaga
     4921 gaagaagcaa caaggttgca aggtgaacta gagaagttga gaaaggaatg gaatgcattg
     4981 gaaaccgaat gccattctct aaaaagggaa aatgttttgc tatcatcaga actgcaacgg
     5041 caagaaaaag aattgcacaa ttctcagaag cagagtttag agcttaccag tgatctcagc
     5101 atccttcaaa tgtctaggaa agaacttgag aatcaagtgg gatccttgaa agaacagcat
     5161 cttcgggatt cagctgattt aaaaactctt ctcagtaagg cagaaaacca agcaaaggat
     5221 gtgcagaaag agtatgaaaa gacacagact gtactctcag aactgaagtt gaagtttgaa
     5281 atgactgagc aggaaaagca gtcaatcaca gatgagctca aacagtgtaa aaacaacctg
     5341 aagctgctcc gagagaaagg aaataatcct tccatattac aacccgtccc agccgtattc
     5401 atcggcctat tcctggcttt cctgttttgg tgtttcggtc cattgtggta ggcggccgcg
     5461 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     5521 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
     5581 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     5641 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     5701 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     5761 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     5821 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
     5881 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     5941 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
     6001 gcctacgcta gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     6061 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6121 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6181 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6241 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6301 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6361 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6421 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6481 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6541 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6601 ccggtgcctg agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6661 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6721 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6781 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6841 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6901 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6961 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     7021 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7081 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7141 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     7201 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7261 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata agatctgctt
     7321 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7381 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7441 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7501 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7561 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     7621 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7681 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     7741 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7801 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7861 gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat catgtcatag
     7921 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7981 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8041 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8101 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8161 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8221 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8281 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8341 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8401 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8461 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8521 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8581 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8641 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8701 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8761 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8821 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8881 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8941 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9001 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9061 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9121 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9181 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9241 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9301 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9361 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9421 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9481 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9541 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9601 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9661 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9721 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9781 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9841 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9901 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9961 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10021 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    10081 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    10141 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    10201 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    10261 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    10321 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    10381 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    10441 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    10501 cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
