LOCUS       sequence-47032-e         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2254
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             76..141
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             148..168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agaacccact ggcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 ctggctagca ccatggacta caaagaccat gacggtgatt ataaagatca tgacatcgat
      121 tacaaggatg acgatgacaa gatggccccc aagaagaaga ggaaggtggg cattcaccgc
      181 ggggtaccta tggtggactt gaggacactc ggttattcgc aacagcaaca ggagaaaatc
      241 aagcctaagg tcaggagcac cgtcgcgcaa caccacgagg cgcttgtggg gcatggcttc
      301 actcatgcgc atattgtcgc gctttcacag caccctgcgg cgcttgggac ggtggctgtc
      361 aaataccaag atatgattgc ggccctgccc gaagccacgc acgaggcaat tgtaggggtc
      421 ggtaaacagt ggtcgggagc gcgagcactt gaggcgctgc tgactgtggc gggtgagctt
      481 agggggcctc cgctccagct cgacaccggg cagctgctga agatcgcgaa gagaggggga
      541 gtaacagcgg tagaggcagt gcacgcctgg cgcaatgcgc tcaccggggc ccccttgaac
      601 ctgaccccag accaggtagt cgcaatcgcg aacaataatg ggggaaagca agccctggaa
      661 accgtgc
//
