LOCUS       sequence-470321-        9281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression of the catalytic-domain (CD) of epigenetic 
            effector Ring1b fused with scFV to programme H2AK119ub..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Policarpi C, Munafo M, Tsagkris S, Carlini V, Hackett JA
  TITLE     Systematic epigenome editing captures the context-dependent 
            instructive function of chromatin modifications.
  JOURNAL   Nat Genet. 2024 Jun;56(6):1168-1180. doi: 
            10.1038/s41588-024-01706-w. Epub 2024 May 9.
  PUBMED    38724747
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-024-01706-w"; journalName: "Nat Genet"; date: 
            "2024-06"; volume: "56"; issue: "6"; pages: "1168-1180"
COMMENT     Sequence Label: pPB_TRE3G_scFV-sfGFP-Ring1bCD
FEATURES             Location/Qualifiers
     source          1..9281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1385..1763
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1393..1411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1429..1447
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1465..1483
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1501..1519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1537..1555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1573..1591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1609..1627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1672..1696
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2520..2546
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2637..3347
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             2637..3347
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(2664..2692)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3287..3307
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3366..3386
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3393..3413
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4179..4300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             4862..5656
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4916..4935)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5526..5545
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5851..5972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   6735..6769
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7260..7276)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7260..7276)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7273..7295)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7284..7300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7308..7314,7315..7332,7333..7338))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7353..7374
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7491..7508)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7662..8250)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7742..7761)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8421..9212,9213..9281))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9044..9063
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtctttcgtc ttcaagaatt
     1381 cctcgagttt actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg
     1441 atagagaacg tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac
     1501 tccctatcag tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta
     1561 tctacagttt actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg
     1621 atagagaacg tataagcttt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt
     1681 agtgaaccgt cagatcgcct ggagcaattc cacaacactt ttgtcttata ccaactttcc
     1741 gtaccacttc ctaccctcgt aaaggtctag agctagccat gggccccgac atcgtgatga
     1801 cccagagccc cagcagcctg agcgccagcg tgggcgaccg cgtgaccatc acctgccgca
     1861 gcagcaccgg cgccgtgacc accagcaact acgccagctg ggtgcaggag aagcccggca
     1921 agctgttcaa gggcctgatc ggcggcacca acaaccgcgc ccccggcgtg cccagccgct
     1981 tcagcggcag cctgatcggc gacaaggcca ccctgaccat cagcagcctg cagcccgagg
     2041 acttcgccac ctacttctgc gccctgtggt acagcaacca ctgggtgttc ggccagggca
     2101 ccaaggtgga gctgaagcgt ggaggcggag gtagtggagg tggaggctct ggtgggggag
     2161 gaagcagtgg aggcggaagt gaggtgaagc tgctggagag cggaggcgga ctggtgcagc
     2221 ccggcggatc tctgaagctg agctgcgccg tgagcggctt cagcctgacc gactacggcg
     2281 tgaactgggt gcgccaggcc cccggacgcg gactggagtg gatcggtgtg atctggggcg
     2341 acggcatcac cgactacaac agcgccctga aggaccgctt catcatcagc aaggacaacg
     2401 gcaagaacac cgtgtacctg cagatgagca aggtgcgcag cgacgacacc gccctgtact
     2461 actgcgtgac cggcctgttc gactactggg gacagggcac cctggtgacc gtgagcagct
     2521 acccatacga tgttccagat tacgctggtg gaggcggagg ttctggggga ggaggtagtg
     2581 gcggtggtgg ttcaggaggc ggcggaagct tggatccagg tggaggtgga agcggtagca
     2641 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     2701 atgggcacaa attttctgtc cgtggagagg gtgaaggtga tgctacaaac ggaaaactca
     2761 cccttaaatt tatttgcact actggaaaac tacctgttcc gtggccaaca cttgtcacta
     2821 ctctgaccta tggtgttcaa tgcttttccc gttatccgga tcacatgaaa cggcatgact
     2881 ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct ttcaaagatg
     2941 acgggaccta caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta
     3001 tcgagttaaa gggtattgat tttaaagaag atggaaacat tcttggacac aaactcgagt
     3061 acaactttaa ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag
     3121 ctaacttcaa aattcgccac aacgttgaag atggttccgt tcaactagca gaccattatc
     3181 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga
     3241 cacaatctgt cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt
     3301 ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaaggt ggaggtcgga
     3361 ccggccccaa gaaaaaacgc aaggtggaag atcctaagaa aaagcggaaa gtggacggca
     3421 ttggtagtgg gagcaacggc agcagcggat ccggaggcgg tggtacctct caggctgtgc
     3481 agacaaatgg aactcaacca ttaagcaaaa catgggaact cagtttgtat gagttacaac
     3541 gaacacctca ggaggcaata acagatggct tggaaattgt ggtttcacct agaagtctac
     3601 acagtgaatt aatgtgccca atttgtttgg atatgttaaa gaacaccatg actacaaagg
     3661 agtgtttaca tcggttttgc gcggattgta ttatcacagc ccttagaagt ggcaacaaag
     3721 agtgtcctac ctgtcggaaa aaactggttt ctaaaagatc actaaggcca gacccgaact
     3781 ttgatgcact catcagcaag atttatccca gtcgtgatga gtatgaagcg catcaggaaa
     3841 gggtcttagc aaggatcaac aaacacaaca atcagcaggc tctcagccac agcatcgagg
     3901 aggggctgaa gatacaggcc atgaacagat tacagcgagg caaaaagcag cagatagaaa
     3961 atggtagtgg agcagaagat aatggtgaca gctcccactg tagtaacgca tccacacaca
     4021 gcaaccagga agcgggcccg agtaacaaac ggaccaaaac ctcttgacaa aagatcataa
     4081 tcagccatat cacatctgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     4141 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     4201 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4261 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctca tatggccttc
     4321 tgcaggagcg tacagaaccc agggccctgg cacccgtgca gaccctggcc caccccacct
     4381 gggcgctcag tgcccaagag atgtccacac ctaggatgtc ccgcggtggg tggggggccc
     4441 gagagacggg caggccgggg gcaggcctgg ccatgcgggg ccgaaccggg cactgcccag
     4501 cgtggggcgc gggggccacg gcgcgcgccc ccagcccccg ggcccagcac cccaaggcgg
     4561 ccaacgccaa aactctccct cctcctcttc ctcaatctcg ctctcgctct tttttttttt
     4621 cgcaaaagga ggggagaggg ggtaaaaaaa tgctgcactg tgcggcgaag ccggtgagtg
     4681 agcggcgcgg ggccaatcag cgtgcgccgt tccgaaagtt gccttttatg gctcgagcgg
     4741 ccgcggcggc gccctataaa acccagcggc gcgacgcgcc accaccgccg agaccgcgtc
     4801 cgccccgcga gcacagagcc tcgcctttgc cgatcctcta gagtcgagat ccgccgccac
     4861 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4921 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4981 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5041 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5101 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5161 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5221 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5281 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5341 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5401 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5461 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5521 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5581 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5641 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5701 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5761 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5821 gcccaccccc cggatctaag ctctagagtt aacttgttta ttgcagctta taatggttac
     5881 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5941 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggaattgact caaatgatgt
     6001 caattagtct atcagaagct catctggtct cccttccggg ggacaagaca tccctgttta
     6061 atatttaaac agcagtgttc ccaaactggg ttcttatatc ccttgctctg gtcaaccagg
     6121 ttgcagggtt tcctgtcctc acaggaacga agtccctaaa gaaacagtgg cagccaggtt
     6181 tagccccgga attgactgga ttcctttttt agggcccatt ggtatggctt tttccccgta
     6241 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
     6301 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
     6361 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
     6421 gtaattacat ccctgggggc tttggggggg ggctgtccct gatatctata acaagaaaat
     6481 atatatataa taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga
     6541 gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt
     6601 atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc
     6661 ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca
     6721 atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt taatctagct
     6781 gcatcaggat catatcgtcg ggtctttttt ccggctcagt catcgcccaa gctggcgcta
     6841 tctgggcatc ggggaggaag aagcccgtgc cttttcccgc gaggttgaag cggcatggaa
     6901 agagtttgcc gaggatgact gctgctgcat tgacgttgag cgaaaacgca cgtttaccat
     6961 gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg ttcaggccac
     7021 ctgggatacc agttcgtcgc ggcttttccg gacacagttc cggatggtca gcccgaagcg
     7081 catcagcaac ccgaacaata ccggcgacag ccggaactgc cgtgccggtg tgcagattaa
     7141 tgacagcggt gcggcgctgg gatattacgt cagcgaggac gggtatcctg gctggatgcc
     7201 gcagaaatgg acatggatac cccgtgagtt acccggcggg cgcgcttggc gtaatcatgg
     7261 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7321 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7381 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7441 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7501 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7561 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7621 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7681 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7741 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7801 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7861 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7921 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7981 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8041 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8101 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8161 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8221 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8281 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8341 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8401 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8461 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8521 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8581 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8641 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8701 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8761 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8821 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8881 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8941 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9001 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9061 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9121 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9181 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9241 aaaaagggaa taagggcgac acggaaatgt tgaatactca t
//
