LOCUS       sequence-470326-       10318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression of the catalytic-domain (CD) of epigenetic 
            effector Dnmt3a3L fused with scFV to programme DNA methylation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Policarpi C, Munafo M, Tsagkris S, Carlini V, Hackett JA
  TITLE     Systematic epigenome editing captures the context-dependent 
            instructive function of chromatin modifications.
  JOURNAL   Nat Genet. 2024 Jun;56(6):1168-1180. doi: 
            10.1038/s41588-024-01706-w. Epub 2024 May 9.
  PUBMED    38724747
REFERENCE   2  (bases 1 to 10318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-024-01706-w"; journalName: "Nat Genet"; date: 
            "2024-06"; volume: "56"; issue: "6"; pages: "1168-1180"
COMMENT     Sequence Label: pPB_TRE3G_scFV-sfGFP-Dnmt3a3lCD
FEATURES             Location/Qualifiers
     source          1..10318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1385..1763
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1393..1411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1429..1447
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1465..1483
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1501..1519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1537..1555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1573..1591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1609..1627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1672..1696
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2520..2546
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2637..3347
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             2637..3347
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(2664..2692)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3287..3307
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3366..3386
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3393..3413
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5216..5337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             5899..6693
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5953..5972)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6563..6582
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6888..7009
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7772..7806
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8297..8313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8297..8313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8310..8332)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8321..8337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8345..8351,8352..8369,8370..8375))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8390..8411
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8528..8545)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8699..9287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8779..8798)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9458..10249,10250..10318))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10081..10100
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtctttcgtc ttcaagaatt
     1381 cctcgagttt actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg
     1441 atagagaacg tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac
     1501 tccctatcag tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta
     1561 tctacagttt actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg
     1621 atagagaacg tataagcttt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt
     1681 agtgaaccgt cagatcgcct ggagcaattc cacaacactt ttgtcttata ccaactttcc
     1741 gtaccacttc ctaccctcgt aaaggtctag agctagccat gggccccgac atcgtgatga
     1801 cccagagccc cagcagcctg agcgccagcg tgggcgaccg cgtgaccatc acctgccgca
     1861 gcagcaccgg cgccgtgacc accagcaact acgccagctg ggtgcaggag aagcccggca
     1921 agctgttcaa gggcctgatc ggcggcacca acaaccgcgc ccccggcgtg cccagccgct
     1981 tcagcggcag cctgatcggc gacaaggcca ccctgaccat cagcagcctg cagcccgagg
     2041 acttcgccac ctacttctgc gccctgtggt acagcaacca ctgggtgttc ggccagggca
     2101 ccaaggtgga gctgaagcgt ggaggcggag gtagtggagg tggaggctct ggtgggggag
     2161 gaagcagtgg aggcggaagt gaggtgaagc tgctggagag cggaggcgga ctggtgcagc
     2221 ccggcggatc tctgaagctg agctgcgccg tgagcggctt cagcctgacc gactacggcg
     2281 tgaactgggt gcgccaggcc cccggacgcg gactggagtg gatcggtgtg atctggggcg
     2341 acggcatcac cgactacaac agcgccctga aggaccgctt catcatcagc aaggacaacg
     2401 gcaagaacac cgtgtacctg cagatgagca aggtgcgcag cgacgacacc gccctgtact
     2461 actgcgtgac cggcctgttc gactactggg gacagggcac cctggtgacc gtgagcagct
     2521 acccatacga tgttccagat tacgctggtg gaggcggagg ttctggggga ggaggtagtg
     2581 gcggtggtgg ttcaggaggc ggcggaagct tggatccagg tggaggtgga agcggtagca
     2641 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     2701 atgggcacaa attttctgtc cgtggagagg gtgaaggtga tgctacaaac ggaaaactca
     2761 cccttaaatt tatttgcact actggaaaac tacctgttcc gtggccaaca cttgtcacta
     2821 ctctgaccta tggtgttcaa tgcttttccc gttatccgga tcacatgaaa cggcatgact
     2881 ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct ttcaaagatg
     2941 acgggaccta caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta
     3001 tcgagttaaa gggtattgat tttaaagaag atggaaacat tcttggacac aaactcgagt
     3061 acaactttaa ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag
     3121 ctaacttcaa aattcgccac aacgttgaag atggttccgt tcaactagca gaccattatc
     3181 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga
     3241 cacaatctgt cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt
     3301 ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaaggt ggaggtcgga
     3361 ccggccccaa gaaaaaacgc aaggtggaag atcctaagaa aaagcggaaa gtggacggca
     3421 ttggtagtgg gagcaacggc agcagcggat ccggaggcgg tggtaccaac catgaccagg
     3481 aatttgaccc cccaaaggtt tacccacctg tgccagctga gaagaggaag cccatccgcg
     3541 tgctgtctct ctttgatggg attgctacag ggctcctggt gctgaaggac ctgggcatcc
     3601 aagtggaccg ctacattgcc tccgaggtgt gtgaggactc catcacggtg ggcatggtgc
     3661 ggcaccaggg aaagatcatg tacgtcgggg acgtccgcag cgtcacacag aagcatatcc
     3721 aggagtgggg cccattcgac ctggtgattg gaggcagtcc ctgcaatgac ctctccattg
     3781 tcaaccctgc ccgcaaggga ctttatgagg gtactggccg cctcttcttt gagttctacc
     3841 gcctcctgca tgatgcgcgg cccaaggagg gagatgatcg ccccttcttc tggctctttg
     3901 agaatgtggt ggccatgggc gttagtgaca agagggacat ctcgcgattt cttgagtcta
     3961 accccgtgat gattgacgcc aaagaagtgt ctgctgcaca cagggcccgt tacttctggg
     4021 gtaaccttcc tggcatgaac aggcctttgg catccactgt gaatgataag ctggagctgc
     4081 aagagtgtct ggagcacggc agaatagcca agttcagcaa agtgaggacc attaccacca
     4141 ggtcaaactc tataaagcag ggcaaagacc agcatttccc cgtcttcatg aacgagaagg
     4201 aggacatcct gtggtgcact gaaatggaaa gggtgtttgg cttccccgtc cactacacag
     4261 acgtctccaa catgagccgc ttggcgaggc agagactgct gggccgatcg tggagcgtgc
     4321 cggtcatccg ccacctcttc gctccgctga aggaatattt tgcttgtgtg tctagcggca
     4381 atagtaacgc taacagccgc gggccgagct tcagcagcgg cctggtgccg ttaagcttgc
     4441 gcggcagcca tatgggccct atggagatat acaagacagt gtctgcatgg aagagacagc
     4501 cagtgcgggt actgagcctt tttagaaata ttgataaagt actaaagagt ttgggctttt
     4561 tagaaagcgg ttctggttct gggggaggaa cgctgaagta cgtggaagat gtcacaaatg
     4621 tcgtgaggag agacgtggag aaatggggcc cctttgacct ggtgtacggc tcgacgcagc
     4681 ccctaggcag ctcctgtgat cgctgtcccg gctggtacat gttccagttc caccggatcc
     4741 tgcagtatgc gctgcctcgc caggagagtc agcggccctt cttctggata ttcatggaca
     4801 atctgctgct gactgaggat gaccaagaga caactacccg cttccttcag acagaggctg
     4861 tgaccctcca ggatgtccgt ggcagggact accagaatgc tatgcgggtg tggagcaaca
     4921 ttccagggct gaagagcaag catgcgcccc tgaccccaaa ggaagaagag tatctgcaag
     4981 cccaagtcag aagcaggagc aagctggacg ccccgaaagt tgacctcctg gtgaagaact
     5041 gccttctccc gctgagagag tacttcaagt atttttctca aaactcactt cctctttagg
     5101 gtaccaaaag atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa
     5161 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     5221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5341 tgtctcatat ggccttctgc aggagcgtac agaacccagg gccctggcac ccgtgcagac
     5401 cctggcccac cccacctggg cgctcagtgc ccaagagatg tccacaccta ggatgtcccg
     5461 cggtgggtgg ggggcccgag agacgggcag gccgggggca ggcctggcca tgcggggccg
     5521 aaccgggcac tgcccagcgt ggggcgcggg ggccacggcg cgcgccccca gcccccgggc
     5581 ccagcacccc aaggcggcca acgccaaaac tctccctcct cctcttcctc aatctcgctc
     5641 tcgctctttt tttttttcgc aaaaggaggg gagagggggt aaaaaaatgc tgcactgtgc
     5701 ggcgaagccg gtgagtgagc ggcgcggggc caatcagcgt gcgccgttcc gaaagttgcc
     5761 ttttatggct cgagcggccg cggcggcgcc ctataaaacc cagcggcgcg acgcgccacc
     5821 accgccgaga ccgcgtccgc cccgcgagca cagagcctcg cctttgccga tcctctagag
     5881 tcgagatccg ccgccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct
     5941 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     6001 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     6061 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     6121 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     6181 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     6241 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     6301 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     6361 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     6421 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     6481 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     6541 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     6601 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     6661 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     6721 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     6781 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     6841 tcatgctgga gttcttcgcc caccccccgg atctaagctc tagagttaac ttgtttattg
     6901 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6961 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     7021 attgactcaa atgatgtcaa ttagtctatc agaagctcat ctggtctccc ttccggggga
     7081 caagacatcc ctgtttaata tttaaacagc agtgttccca aactgggttc ttatatccct
     7141 tgctctggtc aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa
     7201 acagtggcag ccaggtttag ccccggaatt gactggattc cttttttagg gcccattggt
     7261 atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt
     7321 cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg
     7381 ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc
     7441 cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc tgtccctgat
     7501 atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca
     7561 atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt
     7621 tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc
     7681 tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta
     7741 tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt
     7801 ctagggttaa tctagctgca tcaggatcat atcgtcgggt cttttttccg gctcagtcat
     7861 cgcccaagct ggcgctatct gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag
     7921 gttgaagcgg catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga
     7981 aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa
     8041 ctgttcgttc aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg
     8101 atggtcagcc cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt
     8161 gccggtgtgc agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg
     8221 tatcctggct ggatgccgca gaaatggaca tggatacccc gtgagttacc cggcgggcgc
     8281 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8341 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8401 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8461 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8521 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8581 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8641 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8701 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8761 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8821 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8881 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8941 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9001 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9061 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9121 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9181 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9241 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9301 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9361 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9421 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9481 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9541 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9601 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9661 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9721 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9781 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9841 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9901 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9961 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10021 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10081 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10141 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10201 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10261 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcat
//
