LOCUS       sequence-470347-       14131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into 
            TRV2 Architecture_B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14131)
  AUTHORS   Weiss T, Kamalu M, Shi H, Li Z, Amerasekera J, Zhong Z, Adler BA, 
            Song MM, Vohra K, Wirnowski G, Chitkara S, Ambrose C, Steinmetz N, 
            Sridharan A, Sahagun D, Banfield JF, Doudna JA, Jacobsen SE
  TITLE     Viral delivery of an RNA-guided genome editor for transgene-free 
            germline editing in Arabidopsis.
  JOURNAL   Nat Plants. 2025 Apr 22. doi: 10.1038/s41477-025-01989-9.
  PUBMED    40263581
REFERENCE   2  (bases 1 to 14131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2025 Apr 22. doi: 10.1038/s41477-025-01989-9."
COMMENT     Sequence Label: pMK435
FEATURES             Location/Qualifiers
     source          1..14131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    253..277
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        806..1151
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1094..1114
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2728..2775
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             2782..2802
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4125..4249
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4274..4279,4280..4297,4298..4304)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4358..5038
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             5358..5663
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPASVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5542..5561
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5704..5828)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           5840..5907
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             6440..6457
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      6492..6744
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6766..6790
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8091..8720
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9149..10222
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10288..10482
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10718..10740)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10826..10966
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10881..10900
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10981..10998)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11152..11740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11232..11251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13165..13959)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
       61 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
      121 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
      181 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
      241 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
      301 taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc ctaggccacc
      361 atgttgggcc cggcgcgcca agcttgcatg cctgcaggtc aacatggtgg agcacgacac
      421 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac
      481 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
      541 cttcatcgaa aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa
      601 aggaaaggct atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc
      661 cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
      721 atgtgatggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
      781 cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
      841 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
      901 atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctcta
      961 ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa aaagaagacg
     1021 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
     1081 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     1141 tggagaggat aaaacattgc acctatggtg ttgccctggc tggggtatgt cagtgatcgc
     1201 agtagaatgt actaattgac aagttggaga atacggtaga acgtccttat ccaacacagc
     1261 ctttatccct ctccctgacg aggtttttgt cagtgtaata tttctttttg aactatccag
     1321 cttagtaccg tacgggaaag tgactggtgt gcttatcttt gaaatgttac tttgggtttc
     1381 ggttctttag gttagtaaga aagcacttgt cttctcatac aaaggaaaac ctgagacgta
     1441 tcgcttacga aagtagcaat gaaagaaagg tggtggtttt aatcgctacc gcaaaaacga
     1501 tggggtcgtt ttaattaact tctcctacgc aagcgtctaa acggacgttg gggttttgct
     1561 agtttcttta gagaaaacta gctaagtctt taatgttatc attagagatg gcataaatat
     1621 aatacttgtg tctgctgata agatcatttt aatttggacg attagacttg ttgaactaca
     1681 ggttactgaa tcacttgcgc taatcaacat gggagatatg tacgatgaat catttgacaa
     1741 gtcgggcggt cctgctgact tgatggacga ttcttgggtg gaatcagttt cgtggaaaga
     1801 tctgttgaag aagttacaca gcataaaatt tgcactacag tctggtagag atgagatcac
     1861 tgggttacta gcggcactga atagacagtg tccttattca ccatatgagc agtttccaga
     1921 taagaaggtg tatttccttt tagactcacg ggctaacagt gctcttggtg tgattcagaa
     1981 cgcttcagcg ttcaagagac gagctgatga gaagaatgca gtggcgggtg ttacaaatat
     2041 tcctgcgaat ccaaacacaa cggttacgac gaaccaaggg agtactacta ctaccaaggc
     2101 gaacactggc tcgactttgg aagaagactt gtacacttat tacaaattcg atgatgcctc
     2161 tacagctttc cacaaatctc taacttcgtt agagaacatg gagttgaaga gttattaccg
     2221 aaggaacttt gagaaagtat tcgggattaa gtttggtgga gcagctgcta gttcatctgc
     2281 accgcctcca gcgagtggag gtccgatacg tcctaatccc tagggattta aggacgtgaa
     2341 ctctgttgag atctctgtga aattcagagg gtgggtgata ccatattcac tgatgccatt
     2401 agcgacatct aaatagggct aattgtgact aatttgaggg aatttccttt accattgacg
     2461 tcagtgtcgt tggtagcatt tgagtttcgg cacacaaggt taaaaacgct gtagtaatac
     2521 atgcgcaaga acaggctgag catcttgttc tggggtttca cactatcttt agagaaagtg
     2581 ttaagttaat taagttatct taattaagag cataattata ctgatttgtc tctcgttgat
     2641 agagtctatc attctgttat taaaaatttg acaactcggt ttgctgacct actggtcact
     2701 gtatcactta cccgagttaa cgagatggat tacaaggatg atgatgataa ggattacaag
     2761 gatgatgatg ataagatggc tccaaagaag aagagaaagg ttggaatcca cggagttcca
     2821 gctgctatgc tgcagcataa agcctatgaa taccgtatct atccagataa gaagcaggaa
     2881 acactgattg ccaagacaat aggcagttca aggtatgtat acaaccattt cctggaactt
     2941 tggaacaagg aatatgagga gaccggaaaa ggtctgactt attacgcatg ctccaaactt
     3001 ctgacaaagc ttaagaggga cccggaaaca gtatggcttt gtgaagtgga taagttctcg
     3061 ctccagaatt cgcttcgtaa cctgtcggac gccttctccc gctttttcaa aggacagaac
     3121 gagcatcccc agttcaagag caagaagagc ccgaggcaaa gctacacgac acaatatacg
     3181 aacaacaaca tcgcagtttc cggaaattgt ctgaagctgc ctaagctggg ccttgtcaag
     3241 tttgcagaca gtagggagat gaaaggccgt atactgaatg ccacagtacg aaggaaatcg
     3301 agcgggaaat tcttcgtatc gatcctatgc gaagaggaga tctgtgaatt gccaaagact
     3361 gactcatctg tcggaattga cctcggaatc attgactttg cggttatgtc agacggaagc
     3421 aggcatgaca acaatcattt taccaggcaa atggaagaaa ggcttagacg ggagcagcga
     3481 aagcttgcaa ggcgtgcact tgctgcggag aaaaggggca tttccctttc tgaagccagg
     3541 aactatcaga agcaaaggcg gaaggtagcg agactttatg aaaaggttgc aaaccagcga
     3601 aaagaatatc ttaacaaact cagtacagag atagtcaaaa accacgatat catctgtatc
     3661 gaggacctta acgttaaggg catgatgcgc aatcataaac tggcaaaaag catctctgat
     3721 gtatcatgga cgagccttgt atcgaaactg cagtacaagg cttcctggta tggaaaagaa
     3781 gtcatcagga taagcagatg gtttccgtca agtcagatat gctcagagtg cggccacaag
     3841 gacaggaaga agcctctcca tgtaagggag tggacctgtc ctgtttgtca tgcccaccat
     3901 gaccgtgacg tcaatgcagc aagaaatata ctggctgagg gactcaggat aagagctctg
     3961 actccggggt cttagcaata cagaagcaaa caggaaccgc aggaattgcg ggggtagctt
     4021 ggtaaacaag agaaacctct gccggcaaag aaataagccg gtaagtatgc tctgttccca
     4081 agaatctcgt gactttagtc atgagagttt caacatcgca ggtgacaagt ttgtacaaaa
     4141 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
     4201 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt
     4261 aggaacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
     4321 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     4381 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     4441 cgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     4501 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     4561 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     4621 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     4681 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     4741 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     4801 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     4861 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     4921 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     4981 acaacagtac tgcgatgagt ggcaggcggg gcgtaatcta gaggatccgg cttactaaaa
     5041 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     5101 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     5161 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     5221 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     5281 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     5341 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     5401 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     5461 ccctggccag tgcacgtctg ctgtcagata aagtcccccg tgaactttac ccggtggtgc
     5521 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggcctccg
     5581 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     5641 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     5701 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     5761 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc ttttttgtac
     5821 aaacttgtca cctgcgatgg gccggcatgg tcccagcctc ctcgctggcg ccggctgggc
     5881 aacatgcttc ggcatggcga atgggacccg tagctcagtt ggttagagcg ttggtcttat
     5941 gagccgaagg tcgcgggttc gagccccgcc ggaagcaccc gggcatgtcc cgaagacatt
     6001 aaactacggt tctttaagta gatccgtgtc tgaagtttta ggttcaattt aaacctacga
     6061 gattgacatt ctcgactgat cttgattgat cggtaagtct tttgtaattt aattttcttt
     6121 ttgattttat tttaaattgt tatctgtttc tgtgtataga ctgtttgaga tcggcgtttg
     6181 gccgactcat tgtcttacca taggggaacg gactttgttt gtgttgttat tttatttgta
     6241 ttttattaaa attctcaacg atctgaaaaa gcctcgcggc taagagattg ttggggggtg
     6301 agtaagtact tttaaagtga tgatggttac aaaggcaaaa ggggtaaaac ccctcgccta
     6361 cgtaagcgtt attacgcccg tctgtactta tatcagtaca ctgacgagtc cctaaaggac
     6421 gaaacgggag aacgctagcc accaccacca ccaccacgtg tgaattacag gtgaccagct
     6481 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     6541 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     6601 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     6661 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     6721 cggtgtcatc tatgttacta gatcgggaat taaactatca gtgtttgaca ggatatattg
     6781 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
     6841 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
     6901 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
     6961 gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
     7021 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
     7081 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
     7141 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
     7201 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
     7261 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
     7321 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
     7381 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
     7441 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
     7501 gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac
     7561 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
     7621 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
     7681 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
     7741 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
     7801 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
     7861 gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg
     7921 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc
     7981 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     8041 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     8101 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
     8161 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     8221 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     8281 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     8341 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     8401 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     8461 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     8521 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     8581 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     8641 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     8701 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     8761 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     8821 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     8881 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
     8941 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     9001 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
     9061 gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca
     9121 ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca
     9181 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
     9241 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     9301 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     9361 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     9421 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     9481 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     9541 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     9601 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     9661 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     9721 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     9781 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     9841 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     9901 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     9961 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
    10021 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
    10081 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
    10141 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
    10201 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
    10261 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc
    10321 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
    10381 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
    10441 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
    10501 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
    10561 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
    10621 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
    10681 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    10741 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10801 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
    10861 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10921 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
    10981 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    11041 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    11101 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    11161 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    11221 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    11281 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    11341 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    11401 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    11461 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    11521 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    11581 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    11641 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    11701 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    11761 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggctgatga atcccctaat
    11821 gatttttatc aaaatcatta agttaaggta gatacacatc ttgtcatatg atcaaatggt
    11881 ttcgccaaaa atcaataatc agacaacaaa atgtgcgaac tcgatatttt acacgactct
    11941 ctttaccaat tctgccccga attacactta aaacgactca acagcttaac gttggcttgc
    12001 cacgccttac ttgactgtaa aactctcact cttaccgaac ttggccgtaa cctgccaacc
    12061 aaagcgagaa caaaacataa catcaaacga atcgaccgat tgttaggtaa tcgtcacctc
    12121 cacaaagagc gactcgctgt ataccgttgg catgctagct ttatctgttc gggcaatacg
    12181 atgcccattg tacttgttga ctggtctgat atccgtgagc aaaaacggct tatggtattg
    12241 cgagcttcag tcgcactaca cggtcgttct gttactcttt atgagaaagc gttcccgctt
    12301 tcagagcaat gttcaaagaa agctcatgac caatttctag ccgaccttgc gagcattcta
    12361 ccgagtaaca ccacaccgct cattgtcagt gatgctggct ttaaagtgcc atggtataaa
    12421 tccgttgaga agctgggttg gtactggtta agtcgagtaa gaggaaaagt acaatatgca
    12481 gacctaggag cggaaaactg gaaacctatc agcaacttac atgatatgtc atctagtcac
    12541 tcaaagactt taggctataa gaggctgact aaaagcaatc caatctcatg ccaaattcta
    12601 ttgtataaat ctcgctctaa aggccgaaaa aatcagcgct cgacacggac tcattgtcac
    12661 cacccgtcac ctaaaatcta ctcagcgtcg gcaaaggagc catggattct agcaactaac
    12721 ttacctgttg aaattcgaac acccaaacaa cttgttaata tctattcgaa gcgaatgcag
    12781 attgaagaaa ccttccgaga cttgaaaagt cctgcctacg gactaggcct acgccatagc
    12841 cgaacgagca gctcagagcg ttttgatatc atgctgctaa tcgccctgat gcttcaacta
    12901 acatgttggc ttgcgggcgt tcatgctcag aaacaaggtt gggacaagca cttccaggct
    12961 aacacagtca gaaatcgaaa cgtactctca acagttcgct taggcatgga agttttgcgg
    13021 cattctggct acacaataac aagggaagac tcactcgtgg ctgcaaccct gcttactcaa
    13081 aatctattca cacatggtta cgttttgggg aaattatgag gggatctctc agcgttaagg
    13141 gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat
    13201 tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc
    13261 cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct
    13321 gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct
    13381 gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa
    13441 atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac
    13501 atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt
    13561 cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag
    13621 ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc
    13681 ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg
    13741 aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt
    13801 ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag
    13861 cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat
    13921 cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt
    13981 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg
    14041 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag
    14101 ttggcgtata acatagtatc gacggagccg a
//
