LOCUS       sequence-470356-       10001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression of the catalytic-domain (CD) of epigenetic 
            effector Dot1l fused with scFV to programme H3K79me2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10001)
  AUTHORS   Policarpi C, Munafo M, Tsagkris S, Carlini V, Hackett JA
  TITLE     Systematic epigenome editing captures the context-dependent 
            instructive function of chromatin modifications.
  JOURNAL   Nat Genet. 2024 Jun;56(6):1168-1180. doi: 
            10.1038/s41588-024-01706-w. Epub 2024 May 9.
  PUBMED    38724747
REFERENCE   2  (bases 1 to 10001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-024-01706-w"; journalName: "Nat Genet"; date: 
            "2024-06"; volume: "56"; issue: "6"; pages: "1168-1180"
COMMENT     Sequence Label: pPB_TRE3G_scFV-sfGFP-Dot1LCD
FEATURES             Location/Qualifiers
     source          1..10001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1385..1763
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1393..1411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1429..1447
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1465..1483
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1501..1519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1537..1555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1573..1591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1609..1627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1672..1696
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2520..2546
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2637..3347
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             2637..3347
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(2664..2692)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3287..3307
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3366..3386
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3393..3413
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3465..3491
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     polyA_signal    4899..5020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             5582..6376
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5636..5655)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6246..6265
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6571..6692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7455..7489
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7980..7996)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7980..7996)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7993..8015)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8004..8020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8028..8034,8035..8052,8053..8058))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8073..8094
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8211..8228)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8382..8970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8462..8481)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9141..9932,9933..10001))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9764..9783
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtctttcgtc ttcaagaatt
     1381 cctcgagttt actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg
     1441 atagagaacg tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac
     1501 tccctatcag tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta
     1561 tctacagttt actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg
     1621 atagagaacg tataagcttt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt
     1681 agtgaaccgt cagatcgcct ggagcaattc cacaacactt ttgtcttata ccaactttcc
     1741 gtaccacttc ctaccctcgt aaaggtctag agctagccat gggccccgac atcgtgatga
     1801 cccagagccc cagcagcctg agcgccagcg tgggcgaccg cgtgaccatc acctgccgca
     1861 gcagcaccgg cgccgtgacc accagcaact acgccagctg ggtgcaggag aagcccggca
     1921 agctgttcaa gggcctgatc ggcggcacca acaaccgcgc ccccggcgtg cccagccgct
     1981 tcagcggcag cctgatcggc gacaaggcca ccctgaccat cagcagcctg cagcccgagg
     2041 acttcgccac ctacttctgc gccctgtggt acagcaacca ctgggtgttc ggccagggca
     2101 ccaaggtgga gctgaagcgt ggaggcggag gtagtggagg tggaggctct ggtgggggag
     2161 gaagcagtgg aggcggaagt gaggtgaagc tgctggagag cggaggcgga ctggtgcagc
     2221 ccggcggatc tctgaagctg agctgcgccg tgagcggctt cagcctgacc gactacggcg
     2281 tgaactgggt gcgccaggcc cccggacgcg gactggagtg gatcggtgtg atctggggcg
     2341 acggcatcac cgactacaac agcgccctga aggaccgctt catcatcagc aaggacaacg
     2401 gcaagaacac cgtgtacctg cagatgagca aggtgcgcag cgacgacacc gccctgtact
     2461 actgcgtgac cggcctgttc gactactggg gacagggcac cctggtgacc gtgagcagct
     2521 acccatacga tgttccagat tacgctggtg gaggcggagg ttctggggga ggaggtagtg
     2581 gcggtggtgg ttcaggaggc ggcggaagct tggatccagg tggaggtgga agcggtagca
     2641 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     2701 atgggcacaa attttctgtc cgtggagagg gtgaaggtga tgctacaaac ggaaaactca
     2761 cccttaaatt tatttgcact actggaaaac tacctgttcc gtggccaaca cttgtcacta
     2821 ctctgaccta tggtgttcaa tgcttttccc gttatccgga tcacatgaaa cggcatgact
     2881 ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct ttcaaagatg
     2941 acgggaccta caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta
     3001 tcgagttaaa gggtattgat tttaaagaag atggaaacat tcttggacac aaactcgagt
     3061 acaactttaa ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag
     3121 ctaacttcaa aattcgccac aacgttgaag atggttccgt tcaactagca gaccattatc
     3181 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga
     3241 cacaatctgt cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt
     3301 ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaaggt ggaggtcgga
     3361 ccggccccaa gaaaaaacgc aaggtggaag atcctaagaa aaagcggaaa gtggacggca
     3421 ttggtagtgg gagcaacggc agcagcggat ccggaggcgg tggtcctgct gcaaagcgtg
     3481 ttaagcttga tggcggcgag aagctggagc tgaggctcaa gtcgcctgtg ggcgccgagc
     3541 ccgccgtcta cccgtggccg ctgccggtct acgacaagca ccatgatgct gctcatgaga
     3601 ttattgagac tatccggtgg gtctgcgagg aaatcccaga tctcaagctg gccatggaga
     3661 actatgtcct gatcgactac gacaccaaaa gttttgaaag catgcagcgg ctgtgtgaca
     3721 aatacaaccg ggccatcgac agcatccacc agctgtggaa gggcactaca cagcccatga
     3781 agctgaatac tcggccgtcc aatggacttc tgcggcacat cctgcagcag gtatataacc
     3841 actctgtcac tgacccggag aagcttaaca actacgagcc cttctcccct gaggtgtatg
     3901 gggagacctc ctttgacctg gtggcccaga tgattgatga gatcaagatg acagaggatg
     3961 acctgtttgt cgacctgggc agtggtgtgg ggcaggttgt ccttcaggtt gctgcggcca
     4021 ccaactgcaa acatcactac ggagtggaga aagcggacat cccagccaag tacgcagaga
     4081 ccatggaccg agagttcagg aagtggatga aatggtatgg aaaaaagcat gcagaataca
     4141 cactggaacg aggtgacttc ctctcagagg aatggaggga acggatcgcc aacacgagtg
     4201 ttatatttgt gaataacttt gcctttggtc ctgaggtgga tcaccagctg aaggagcgat
     4261 tcgcaaacat gaaggaaggc ggcagaatcg tatcctcaaa gccctttgcc cctctgaact
     4321 tcaggatcaa cagcaggaac ttgagtgaca ttggcaccat catgcgtgtg gtggagctgt
     4381 cgcccctgaa gggctctgtg tcgtggactg gcaagcctgt ctcctactat ctgcacacca
     4441 ttgaccgcac catacttgaa aactattttt ctagtctgaa aaatccaaaa ctcagggagg
     4501 agcaggaggc agctaggcga agacagcagc gagagaacaa gagcaatgcg accacgccca
     4561 ccaaagtccc tgagagcaaa gcagctgcca ctgaggcccc cgcggattct ggtgctgagg
     4621 aagaaaaatc aggtgtggct actgtcaaaa agccatctcc ctccaaagcc cggaagaaga
     4681 aactgaacaa gaaagggaga aagatggctg gccggaaacg tgggcggccc aagaaaatga
     4741 gtgctgccag tgccgaacgc aagtctaaga agagccaaag atcttgacaa aagatcataa
     4801 tcagccatat cacatctgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     4861 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     4921 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4981 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctca tatggccttc
     5041 tgcaggagcg tacagaaccc agggccctgg cacccgtgca gaccctggcc caccccacct
     5101 gggcgctcag tgcccaagag atgtccacac ctaggatgtc ccgcggtggg tggggggccc
     5161 gagagacggg caggccgggg gcaggcctgg ccatgcgggg ccgaaccggg cactgcccag
     5221 cgtggggcgc gggggccacg gcgcgcgccc ccagcccccg ggcccagcac cccaaggcgg
     5281 ccaacgccaa aactctccct cctcctcttc ctcaatctcg ctctcgctct tttttttttt
     5341 cgcaaaagga ggggagaggg ggtaaaaaaa tgctgcactg tgcggcgaag ccggtgagtg
     5401 agcggcgcgg ggccaatcag cgtgcgccgt tccgaaagtt gccttttatg gctcgagcgg
     5461 ccgcggcggc gccctataaa acccagcggc gcgacgcgcc accaccgccg agaccgcgtc
     5521 cgccccgcga gcacagagcc tcgcctttgc cgatcctcta gagtcgagat ccgccgccac
     5581 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5641 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5701 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5761 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5821 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5881 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5941 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     6001 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     6061 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     6121 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     6181 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     6241 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     6301 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     6361 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     6421 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     6481 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6541 gcccaccccc cggatctaag ctctagagtt aacttgttta ttgcagctta taatggttac
     6601 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6661 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggaattgact caaatgatgt
     6721 caattagtct atcagaagct catctggtct cccttccggg ggacaagaca tccctgttta
     6781 atatttaaac agcagtgttc ccaaactggg ttcttatatc ccttgctctg gtcaaccagg
     6841 ttgcagggtt tcctgtcctc acaggaacga agtccctaaa gaaacagtgg cagccaggtt
     6901 tagccccgga attgactgga ttcctttttt agggcccatt ggtatggctt tttccccgta
     6961 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
     7021 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
     7081 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
     7141 gtaattacat ccctgggggc tttggggggg ggctgtccct gatatctata acaagaaaat
     7201 atatatataa taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga
     7261 gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt
     7321 atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc
     7381 ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca
     7441 atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt taatctagct
     7501 gcatcaggat catatcgtcg ggtctttttt ccggctcagt catcgcccaa gctggcgcta
     7561 tctgggcatc ggggaggaag aagcccgtgc cttttcccgc gaggttgaag cggcatggaa
     7621 agagtttgcc gaggatgact gctgctgcat tgacgttgag cgaaaacgca cgtttaccat
     7681 gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg ttcaggccac
     7741 ctgggatacc agttcgtcgc ggcttttccg gacacagttc cggatggtca gcccgaagcg
     7801 catcagcaac ccgaacaata ccggcgacag ccggaactgc cgtgccggtg tgcagattaa
     7861 tgacagcggt gcggcgctgg gatattacgt cagcgaggac gggtatcctg gctggatgcc
     7921 gcagaaatgg acatggatac cccgtgagtt acccggcggg cgcgcttggc gtaatcatgg
     7981 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8041 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8101 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8161 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8221 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8281 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8341 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8401 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8461 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8521 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8581 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8641 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8701 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8761 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8821 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8881 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8941 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9001 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9061 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9121 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9181 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9241 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9301 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9361 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9421 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9481 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9541 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9601 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9661 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9721 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9781 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9841 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9901 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9961 aaaaagggaa taagggcgac acggaaatgt tgaatactca t
//
