LOCUS       sequence-47036-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Van Engelenburg SB, Nahreini T, Palmer AE
  TITLE     FACS-based selection of tandem tetracysteine peptides with improved 
            ReAsH brightness in live cells.
  JOURNAL   Chembiochem. 2010 Mar 1;11(4):489-93.
  PUBMED    20099291
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem."; date: "2010-03-1"; volume: "11(4)"; pages: "489-93"
COMMENT     Sequence Label: pC2xTC1-alphaTubulin
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             99..116
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             144..161
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttacc atgggcagct tcctgaactg ctgccctggc tgctgcatgg
      121 agcctatcct ggagttcctg aactgctgcc ctggctgctg catggagcct aggcctaggg
      181 gcagccgtga gtgcatctcc atccacgttg gccaggctgg tgtccagatt ggcaatgcct
      241 gctgggagct ctactgcctg gaacacggca tccagcccga tggccagatg ccaagtgaca
      301 agaccattgg gggaggagat gactccttca acaccttctt cagtgagacg ggcgctggca
      361 agcacgtgcc ccgggctgtg tttgtagact tggaacccac agtcattgat gaagttcgca
      421 ctggcaccta ccgccagctc ttccaccctg agcagctcat cacaggcaag gaagatgctg
      481 ccaataacta tgcccgaggg cactacacca ttggcaagga gatcattgac cttgtgttgg
      541 accgaattcg caagctggct gaccagtgca ccggccttca gggcttcttg gttttccaca
      601 gctttggtgg gggaactggt tctgggttca cctccctgct catggaacgt ctctcagttg
      661 attatggcaa gaagtccaag ctggagttct ccatttaccc agcaccccag gtttccacag
      721 ctgtagttga gccctacaac tccatcctca ccacccacac caccctggag cactctgatt
      781 gtgccttcat ggtagacaat gaggccatct atgacatctg tcgtagaaac ctcgatatcg
      841 agcgcc
//
