LOCUS       sequence-470387-        2591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 3-4 fluorescent protein cassette for Golden Gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Lariviere PJ, Zuberi Ashraf AHM, Leonard SP, Miller LG, Moran NA, 
            Barrick JE
  TITLE     Single-step genome engineering in the bee gut symbiont Snodgrassella
            alvi
  JOURNAL   bioRxiv 2023.09.19.558440
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.09.19.558440"
COMMENT     Sequence Label: pBTK1053
FEATURES             Location/Qualifiers
     source          1..2591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             160..834
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     558..577
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      complement(989..1023)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1094..1753)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1668..1687
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1935..2523
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2424..2443
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgcgttatcc cctgattctg tggataaccg tagtcggtct catatgtgaa ctggccgata
       61 attgcagacg aacgctttgg cagtttattc ttgacatgta gtgagggggc tggtataatc
      121 acatagtact gttatacaga aacagaggag atattacata tggatagcac tgagaacgtc
      181 atcaagccct tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      241 gagatcgagg gcgtgggcga gggcaagccc tacgagggca cccagaccgc caagctgcaa
      301 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcttctac
      361 ggctccaagg cgtacatcaa gcaccccgcc gacatccccg actacctcaa gcagtccttc
      421 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
      481 acccaggact cctccctgca ggacggcacc ctcatctacc acgtgaagtt catcggcgtg
      541 aacttcccct ccgacggccc cgtaatgcag aagaagactc tgggctggga gccctccact
      601 gagcgcaact acccccgcga cggcgtgctg aagggcgaga accacatggc gctgaagctg
      661 aagggcggcg gccactacct gtgtgagttc aagtccatct acatggccaa gaagcccgtg
      721 aagctgcccg gctaccacta cgtggactac aagctcgaca tcacctccca caacgaggac
      781 tacaccgtgg tggagcagta cgagcgcgcc gaggcccgcc accacctgtt ccagactcac
      841 ggtatggacg aattgtacaa gcacgacgaa ttgtaaatcc gtaatcgtta atccgcaaat
      901 aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa agagcatttg
      961 tcagctgcgt cgctgtgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct
     1021 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     1081 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     1141 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     1201 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     1261 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa
     1321 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1381 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1441 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1501 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     1561 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1621 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1681 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1741 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     1801 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc
     1861 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1921 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1981 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2041 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2101 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2161 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2221 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2281 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2341 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2401 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2461 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2521 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2581 atgttctttc c
//
