LOCUS       sequence-470394-        9630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional reporter for detecting calcium transients in 
            Drosophila larvae (TEVp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Zhou G, Li R, Bartolik O, Ma Y, Wan WW, Meng J, Hu Y, Ye B, Wang W
  TITLE     An improved FLARE system for recording and manipulating neuronal 
            activity.
  JOURNAL   Cell Rep Methods. 2025 Apr 21;5(4):101012. doi: 
            10.1016/j.crmeth.2025.101012. Epub 2025 Mar 21.
  PUBMED    40120579
REFERENCE   2  (bases 1 to 9630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2025-04-21"; volume: "5(4):101012. doi"; pages: " 
            10.1016/j.crmeth.2025.101012. Epub 2025 Mar 21"
COMMENT     Sequence Label: pUASTattB-cytoFLARE1.0-TEV
FEATURES             Location/Qualifiers
     source          1..9630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     165..184
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        165..183
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(217..235)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(687..758,1160..1433,1508..2162,2224..2539,2743..2874,
                     2945..3559)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4334..4367
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4400..4494
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        4513..4751
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     4698..4719
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             5219..5260
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          6046..6111
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6241..6261
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6533..6667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6570..6589)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6624..6643
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6696..6765
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(6984..7002)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7023..7039)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7023..7039)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7036..7058)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7047..7063
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7071..7077,7078..7095,7096..7101))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7116..7137
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7254..7271)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7425..8013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7505..7524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8184..8975,8976..9044))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8807..8826
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9045..9149)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9175..9630)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9312..9333)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9524..9543
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
      181 agggcgaatt gggtacgtac cgggccccta gtatgtatgt aagttaataa aacccatttt
      241 tgcggaaagt agataaaaaa aacatttttt ttttttactg cactggatat cattgaactt
      301 atctgatcag ttttaaattt acttcgatcc aagggtattt gatgtaccag gttctttcga
      361 ttacctctca ctcaaaatga cattccactc aaagtcagcg ctgtttgcct ccttctctgt
      421 ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg
      481 tagcgttacg tagcgtcaat gtccgccttc agttgcattt tgtcagcggt ttcgtgacga
      541 agctccaagc ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc
      601 ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc
      661 aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta ttaattcgcg
      721 gaggcagcaa acacccatct gccgagcatc tgaacaatgt gagtagtaca tgtgcataca
      781 tcttaagttc acttgatcta taggaactgc gattgcaaca tcaaattgtc tgcggcgtga
      841 gaactgcgac ccacaaaaat cccaaaccgc aattgcacaa acaaatagtg acacgaaaca
      901 gattattctg gtagctgttc tcgctatata agacaatttt tgagatcata tcatgatcaa
      961 gacatctaaa ggcattcatt ttcgactata ttctttttta caaaaaatat aacaaccaga
     1021 tattttaagc tgatcctaga tgcacaaaaa ataaataaaa gtataaacct acttcgtagg
     1081 atacttcggg gtactttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg
     1141 agatccccta tcattgcagg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg
     1201 cttcgggcag gccaaaaact acggcacgct ccggccaccc agtccgccgg aggactccgg
     1261 ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt
     1321 tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact
     1381 attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt
     1441 tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga
     1501 aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt
     1561 gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta
     1621 tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc
     1681 aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac
     1741 ctgattttcc aagccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc
     1801 cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt
     1861 gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc
     1921 gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactcc
     1981 tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc
     2041 atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg
     2101 atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc
     2161 tagtgagttc gatgtgttta ttaagggtat ctagtattac ataacatctc aactcctatc
     2221 cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt
     2281 ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa
     2341 ttttgccatt agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatctgga
     2401 gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt
     2461 ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt
     2521 gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa
     2581 ttcttggaaa caaattcgct agattttaga tagaattgcc tgattccaca cccttcttag
     2641 tttttttcaa tgagatgtat agtttatagt tttgcagaag ataaataaat ttcatttaac
     2701 tcgcgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc catcttgatt
     2761 ggcctcatct ttttgggcca acaactcacg caagtgggtg tgatgaatat caacggagcc
     2821 atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat aaatgtaagt
     2881 catgtttaga atacatttgc atttcaataa tttactaact ttctaatgaa tcgattcgat
     2941 ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc gactttatcg
     3001 ctgtgacaca tactttctgg gcaaaacgat tgccgaattg ccgctttttc tcacagtgcc
     3061 actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag tgctgcactt
     3121 cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct tcggatatct
     3181 aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc cggttatcat
     3241 accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag tatacctcaa
     3301 atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg
     3361 ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc
     3421 gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt
     3481 gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg
     3541 ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt ttttttttac
     3601 cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt aattatattt
     3661 gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc gaacatcccc
     3721 acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca taccttacat
     3781 caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata catacttttc
     3841 aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag ttgctctttc
     3901 gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc ttggagcgac
     3961 tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc atttcgtctc
     4021 aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata tatttgtata
     4081 tatttccctc acagctatat ttattctaat ttaatattat gactttttaa ggtaattttt
     4141 tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat ccagtgtttg
     4201 ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt tatatatatc
     4261 aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg gatttcactg
     4321 gaactaggct agcataactt cgtataatgt atgctatacg aagttatgct agcggatcca
     4381 agcttgcatg cctgcaggtc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
     4441 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggaga
     4501 ctctagcgag cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc
     4561 aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga
     4621 acaagctaaa caatctgcag taaagtgcaa gttaaagtga atcaattaaa agtaaccagc
     4681 aaccaagtaa atcaactgca actactgaaa tctgccaaga agtaattatt gaatacaaga
     4741 agagaactct gaatagggaa ttgggaattc caaaatggac caactgactg aagagcagat
     4801 cgcagaattt aaagaggctt tctccctact ggacaaggac ggggatggga caataacaac
     4861 caaggagctg gggacgggga tgcggtctct ggggcagaac cccacagaag cagagctgca
     4921 ggacatgatc aatgaagtag atgccgacgg tgacggcaca atcgacttcc ctgagttcct
     4981 gacaatgatg gcaagaaaaa tgaaatacag ggacacggaa gaagaaatta gagaagcgtt
     5041 cggtgtgttt gataaggatg gcaatggcta catcagtgca gcagagcttc gccacgtgat
     5101 gacaaacctt ggagagaagt taacagatga agaggttgat gaaatgatca gggaagcaga
     5161 catcgatggg gatggtcagg taaactacga agagtttgta caaatgatga cagcgaaggg
     5221 caagccgatc cctaaccctc tgctgggcct ggacagcact ggtggagcta gcggtggaag
     5281 tggatcaggc agcggtggat ctggagaaag cttgtttaag ggaccacgtg attacaaccc
     5341 gatatcgagc accatttgtc atttgacgaa tgaatctgat gggcacacaa catcgttgta
     5401 tggtattgga tttggtccct tcatcattac aaacaagcac ttgtttagaa gaaataatgg
     5461 aacactgttg gtccaatcac tacatggtgt attcaaggtc aagaacacca cgactttgca
     5521 acaacacctc attgatggga gggacatgat aattattcgc atgcctaagg atttcccacc
     5581 atttcctcaa aagctgaaat ttagagagcc acaaagggaa gagcgcatat gtcttgtgac
     5641 aaccaacttc caaactaaga gcatgtctag catggtgtca gacactagtt gcacattccc
     5701 ttcatctgat ggcatattct ggaagcattg gattcaaacc aaggatgggc agtgtggcaa
     5761 tccattagta tcaactagag atgggttcat tgttggtata cactcagcat cgaatttcac
     5821 caacacaaac aattatttca caagcgtgcc gaaaaacttc atggaattgt tgacaaatca
     5881 ggaggcgcag cagtgggtta gtggttggcg attaaatgct gactcagtat tgtggggtgg
     5941 ccataaagtt ttcatggtgt aatctagagg atctttgtga aggaacctta cttctgtggt
     6001 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     6061 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     6121 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
     6181 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
     6241 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
     6301 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
     6361 gaaaaagctg cactgctata caagaaaatt atggaaaaat atttgatgta tagtgccttg
     6421 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     6481 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     6541 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6601 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6661 ctggatccac tagtgtcgac gatgtaggtc acggtctcga agccgcggtg cgggtgccag
     6721 ggcgtgccct tgggctcccc gggcgcgtac tccacctcac ccatctggtc catcatgatg
     6781 aacgggtcga ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg
     6841 ccctcgaaac cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg
     6901 ggtgcggata cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca
     6961 ctagttctag ccagcttttg ttccctttag tgagggttaa tttcgagctt ggcgtaatca
     7021 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7081 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7141 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7201 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7261 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7321 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7381 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7441 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7501 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7561 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7621 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7681 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7741 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7801 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7861 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7921 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7981 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8041 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8101 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8161 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8221 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8281 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8341 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8401 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8461 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8521 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8581 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8641 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8701 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8761 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8821 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8881 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8941 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9001 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9061 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9121 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     9181 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     9241 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     9301 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     9361 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     9421 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     9481 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     9541 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     9601 ccgcgcttaa tgcgccgcta cagggcgcgt
//
