LOCUS       sequence-470396-       10905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional reporter for detecting calcium transients in 
            Drosophila larvae (transcription factor).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Zhou G, Li R, Bartolik O, Ma Y, Wan WW, Meng J, Hu Y, Ye B, Wang W
  TITLE     An improved FLARE system for recording and manipulating neuronal 
            activity.
  JOURNAL   Cell Rep Methods. 2025 Apr 21;5(4):101012. doi: 
            10.1016/j.crmeth.2025.101012. Epub 2025 Mar 21.
  PUBMED    40120579
REFERENCE   2  (bases 1 to 10905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2025-04-21"; volume: "5(4):101012. doi"; pages: " 
            10.1016/j.crmeth.2025.101012. Epub 2025 Mar 21"
COMMENT     Sequence Label: pUASTattB-cytoFLARE1.0-TF
FEATURES             Location/Qualifiers
     source          1..10905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(29..457)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(139..160)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     351..370
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     584..606
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     598..615
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     599..615
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     622..641
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        622..640
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(674..692)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1144..1215,1617..1890,1965..2619,2681..2996,3200..3331,
                     3402..4016)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4791..4824
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4857..4951
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        4970..5208
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5155..5176
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             5241..6176
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             6789..6809
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             6822..6845
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(6846..7097,7098..7451)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     7389..7410
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             7458..7691
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     intron          7778..7843
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7973..7993
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8265..8399
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8302..8321)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8356..8375
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8428..8497
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(8716..8734)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8755..8771)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8755..8771)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8768..8790)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8779..8795
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8803..8809,8810..8827,8828..8833))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8848..8869
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8986..9003)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9157..9745)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9237..9256)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9916..10707,10708..10776))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10539..10558
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10777..10881)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
       61 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
      121 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
      181 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
      241 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
      301 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
      361 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
      421 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
      481 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      541 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      601 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg tacgtaccgg
      661 gcccctagta tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac
      721 attttttttt tttactgcac tggatatcat tgaacttatc tgatcagttt taaatttact
      781 tcgatccaag ggtatttgat gtaccaggtt ctttcgatta cctctcactc aaaatgacat
      841 tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt
      901 tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacgtag cgtcaatgtc
      961 cgccttcagt tgcattttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc
     1021 aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg
     1081 agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg
     1141 gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc
     1201 gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag
     1261 gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc
     1321 aaaccgcaat tgcacaaaca aatagtgaca cgaaacagat tattctggta gctgttctcg
     1381 ctatataaga caatttttga gatcatatca tgatcaagac atctaaaggc attcattttc
     1441 gactatattc ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc
     1501 acaaaaaata aataaaagta taaacctact tcgtaggata cttcggggta ctttttgttc
     1561 ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca ttgcagggtg
     1621 acagcggagc ggcttcgcag agctgcatta accagggctt cgggcaggcc aaaaactacg
     1681 gcacgctccg gccacccagt ccgccggagg actccggttc agggagcggc caactagccg
     1741 agaacctcac ctatgcctgg cacaatatgg acatctttgg ggcggtcaat cagccgggct
     1801 ccggatggcg gcagctggtc aaccggacac gcggactatt ctgcaacgag cgacacatac
     1861 cggcgcccag gaaacatttg ctcaagaacg gtgagtttct attcgcagtc ggctgatctg
     1921 tgtgaaatct taataaaggg tccaattacc aatttgaaac tcagtttgcg gcgtggccta
     1981 tccgggcgaa cttttggccg tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa
     2041 tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg ccatccggga tgcgactgct
     2101 caatggccaa cctgtggacg ccaaggagat gcaggccagg tgcgcctatg tccagcagga
     2161 tgacctcttt atcggctccc taacggccag ggaacacctg attttccaag ccatggtgcg
     2221 gatgccacga catctgacct atcggcagcg agtggcccgc gtggatcagg tgatccagga
     2281 gctttcgctc agcaaatgtc agcacacgat catcggtgtg cccggcaggg tgaaaggtct
     2341 gtccggcgga gaaaggaagc gtctggcatt cgcctccgag gctctaaccg atccgccgct
     2401 tctgatctgc gatgagccca cctccggact ggactccttt accgcccaca gcgtcgtcca
     2461 ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc atcagccgtc
     2521 ttccgagctg tttgagctct ttgacaagat ccttctgatg gccgagggca gggtagcttt
     2581 cttgggcact cccagcgaag ccgtcgactt cttttcctag tgagttcgat gtgtttatta
     2641 agggtatcta gtattacata acatctcaac tcctatccag cgtgggtgcc cagtgtccta
     2701 ccaactacaa tccggcggac ttttacgtac aggtgttggc cgttgtgccc ggacgggaga
     2761 tcgagtcccg tgatcggatc gccaagatat gcgacaattt tgccattagc aaagtagccc
     2821 gggatatgga gcagttgttg gccaccaaaa atctggagaa gccactggag cagccggaga
     2881 atgggtacac ctacaaggcc acctggttca tgcagttccg ggcggtcctg tggcgatcct
     2941 ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg acttattcag acaacggtga
     3001 gtggttccag tggaaacaaa tgatataacg cttacaattc ttggaaacaa attcgctaga
     3061 ttttagatag aattgcctga ttccacaccc ttcttagttt ttttcaatga gatgtatagt
     3121 ttatagtttt gcagaagata aataaatttc atttaactcg cgaatattaa tgagatgcga
     3181 gtaacatttt aatttgcaga tggttgccat cttgattggc ctcatctttt tgggccaaca
     3241 actcacgcaa gtgggtgtga tgaatatcaa cggagccatc ttcctcttcc tgaccaacat
     3301 gacctttcaa aacgtctttg ccacgataaa tgtaagtcat gtttagaata catttgcatt
     3361 tcaataattt actaactttc taatgaatcg attcgattta ggtgttcacc tcagagctgc
     3421 cagtttttat gagggaggcc cgaagtcgac tttatcgctg tgacacatac tttctgggca
     3481 aaacgattgc cgaattgccg ctttttctca cagtgccact ggtcttcacg gcgattgcct
     3541 atccgatgat cggactgcgg gccggagtgc tgcacttctt caactgcctg gcgctggtca
     3601 ctctggtggc caatgtgtca acgtccttcg gatatctaat atcctgcgcc agctcctcga
     3661 cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc attcctgctc tttggcggct
     3721 tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac ctctcatggt
     3781 tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag ccgggcgaaa
     3841 ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc ctggagacgc
     3901 ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggccatt ctcatcgtga
     3961 gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag gagtagccga
     4021 catatatccg aaataactgc ttgttttttt tttttaccat tattaccatc gtgtttactg
     4081 tttattgccc cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa caagatatga
     4141 cctatagaat acaagtattt ccccttcgaa catccccaca agtagacttt ggatttgtct
     4201 tctaaccaaa agacttacac acctgcatac cttacatcaa aaactcgttt atcgctacat
     4261 aaaacaccgg gatatatttt ttatatacat acttttcaaa tcgcgcgccc tcttcataat
     4321 tcacctccac cacaccacgt ttcgtagttg ctctttcgct gtctcccacc cgctctccgc
     4381 aacacattca ccttttgttc gacgaccttg gagcgactgt cgttagttcc gcgcgattcg
     4441 gttcgctcaa atggttccga gtggttcatt tcgtctcaat agaaattagt aataaatatt
     4501 tgtatgtaca atttatttgc tccaatatat ttgtatatat ttccctcaca gctatattta
     4561 ttctaattta atattatgac tttttaaggt aattttttgt gacctgttcg gagtgattag
     4621 cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga tgcatctcaa
     4681 aaaaatggtg ggcataatag tgttgtttat atatatcaaa aataacaact ataataataa
     4741 gaatacattt aatttagaaa atgcttggat ttcactggaa ctaggctagc ataacttcgt
     4801 ataatgtatg ctatacgaag ttatgctagc ggatccaagc ttgcatgcct gcaggtcgga
     4861 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     4921 ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgagcgc cggagtataa
     4981 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg
     5041 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa
     5101 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact
     5161 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagggaattg
     5221 ggaattccaa aatgccatct gctggagaca tgagagctgc caacctttgg ccaagcccgc
     5281 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
     5341 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
     5401 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
     5461 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
     5521 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
     5581 tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
     5641 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
     5701 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
     5761 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
     5821 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
     5881 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
     5941 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
     6001 tctatgacct gctgctggag gcggcggacg cccaccgcct acatgcgccc actagccgtg
     6061 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
     6121 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagctg
     6181 ctagcttcaa cgctcgcagg aagctgaagg gtgctatcct gttcaccatg ctcgccacaa
     6241 gaaacttcag cggcagcttt aatgcgcggc gcaagctaaa gggagccata cttttcacaa
     6301 tgttggctac cagaaatttt tcggagctgg ccgagaagct ggccggcctg gacatcaacg
     6361 gtggaactag tgagtttcgg gcaaccacac tggaacggat cgagaaaagc ttcgtgatta
     6421 ctgatccgag actgcctgac aacccagtga tttttgtgag cgattccttc ctgcagctga
     6481 cagaatattc tcgggaagag atcctggggc gcaattgccg ttttctgcag ggacccgaga
     6541 cagaccgtgc cactgttcgg aaaatcagag atgctattga caaccagact gaagtgaccg
     6601 ttcagctgat caattatacc aagagcggca agaagttctg gaacgtgttc cacctgcagc
     6661 cgatgcgcga ttataagggc gacgtccagt acttcattgg cgtgcagctg gatggcaccg
     6721 aacgtcttca tggcgccgct gagcgtgagg cggtctgcct ggtcaaaaag acagcctttc
     6781 agattgctga gaacctgtac ttccagatgg gcggaggatc cgactacaag gatgacgacg
     6841 ataagatgaa agcgttaacg gccaggcaac aagaggtgtt tgatctcatc cgtgatcaca
     6901 tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc gcagcgtttg gggttccgtt
     6961 ccccaaacgc ggctgaagaa catctgaagg cgctggcacg caaaggcgtt attgaaattg
     7021 tttccggcgc atcacgcggg attcgtctgt tgcaggaaga ggaagaaggg ttgccgctgg
     7081 taggtcgtgt ggctgccggt gaaccacttc tggcgcaaca gcatattgaa ggtcattatc
     7141 aggtcgatcc ttccttattc aagccgaatg ctgatttcct gctgcgcgtc agcgggatgt
     7201 cgatgaaaga catcggcatt atggatggtg acttgctggc agtgcataaa actcaggatg
     7261 tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga ggttaccgtt aagcgcctga
     7321 aaaaacaggg caataaagtc gaactgttgc cagaaaatag cgagtttaaa ccaattgtcg
     7381 tagatcttcg tcagcagagc ttcaccattg aagggctggc ggttggggtt attcgcaacg
     7441 gcgactggct gggcggcgcc cccccgaccg atgtcagcct gggggacgaa ttgcacttag
     7501 acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt
     7561 tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg
     7621 ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg
     7681 agtacggtgg gtaatctaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     7741 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     7801 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     7861 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     7921 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     7981 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     8041 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     8101 tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga
     8161 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     8221 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     8281 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8341 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     8401 actagtgtcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc
     8461 cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga tgaacgggtc
     8521 gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa
     8581 accgctgggc gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg cgggtgcgga
     8641 tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga cactagttct
     8701 agccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
     8761 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8821 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8881 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8941 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9001 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9061 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9121 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9181 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9241 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9301 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9361 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9421 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9481 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9541 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9601 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9661 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9721 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9781 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9841 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9901 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9961 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10021 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10081 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10141 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10201 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10261 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10321 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10381 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10441 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10501 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10561 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10621 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10681 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10741 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10801 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10861 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacc
//
