LOCUS       sequence-470431-       16960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of HaloTag-DNA-PKcs in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16960)
  AUTHORS   Mikhova M, Goff NJ, Janovic T, Heyza JR, Meek K, Schmidt JC
  TITLE     Single-molecule imaging reveals the kinetics of non-homologous 
            end-joining in living cells.
  JOURNAL   Nat Commun. 2024 Nov 23;15(1):10159. doi: 
            10.1038/s41467-024-54545-y.
  PUBMED    39578493
REFERENCE   2  (bases 1 to 16960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-11-23"; volume: "15(1):10159. doi"; pages: " 
            10.1038/s41467-024-54545-y"
COMMENT     Sequence Label: Halo-DNAPKcs
FEATURES             Location/Qualifiers
     source          1..16960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..37)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        105..209
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(210..278,279..1070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(428..447)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1241..1829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1730..1749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1983..2000
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2065..2087
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2084..2100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2084..2100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2134..2167
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        2311..2614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2620..2897
                     /label=chicken beta-actin promoter
     CDS             3049..3114
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    3115..3148
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3157..4047
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             4060..4080
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(16534..16551)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    16540..16764
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(16802..16835)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(16868..16884)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16917..16933)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16926..16948)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
       61 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      181 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      421 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
      481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
      661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
      721 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
      781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
      841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
      901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
      961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1381 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1621 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     1921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1981 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     2041 atctgtgcgg tatttcacac cgcaagcgga taacaatttc acacaggaaa cagctatgac
     2101 catgattacg ccaagcttgc atgcctgcag gggataactt cgtataatgt atgctatacg
     2161 aagttatggg gccgctctag cctcgaggcc gtaatgagac gcacaaacta atatcacaaa
     2221 ctggaaatgt ctatcaatat atagttgctc tagttattaa tagtaatcaa ttacggggtc
     2281 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2341 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2401 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2461 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2521 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2581 gtacatctac gtattagtca tcgctattac catgcatggt cgaggtgagc cccacgttct
     2641 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     2701 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg
     2761 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
     2821 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     2881 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
     2941 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     3001 gggacggccc ttctccttcg ggctgtaatt agcatcacgc gtaccatgga ctacaaagac
     3061 catgacggtg attataaaga tcatgatatc gattacaagg atgacgatga caagataact
     3121 tcgtataatg tatgctatac gaagttatcc ggtaccatgg cagaaatcgg tactggcttt
     3181 ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt cgatgttggt
     3241 ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc ctacgtgtgg
     3301 cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga cctgatcggt
     3361 atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt ccgcttcatg
     3421 gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca cgactggggc
     3481 tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg tattgcattt
     3541 atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc ccgcgagacc
     3601 ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca gaacgttttt
     3661 atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga gatggaccat
     3721 taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt cccaaacgag
     3781 ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata catggactgg
     3841 ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt tctgatccca
     3901 ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt ggacatcggc
     3961 ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga gatcgcgcgc
     4021 tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta ctttcagagc
     4081 gataacgcga tcgcttccgg taccatggca ggaagtggag caggtgtgag atgcagtcta
     4141 ctaagactac aagaaacctt aagtgctgcg gaccgctgcg gtgctgccct ggccggtcat
     4201 caactgatcc gcggcctggg gcaggaatgc gtcctgagca gcagccccgc ggtgctggca
     4261 ttacagacat ctttagtttt ttccagagat ttcggtttgc ttgtatttgt ccggaagtca
     4321 ctcaacagta ttgaatttcg tgaatgtaga gaagaaatcc taaagttttt atgtattttc
     4381 ttagaaaaaa tgggccagaa gatcgcacct tactctgttg aaattaagaa cacttgtacc
     4441 agtgtttata caaaagatag agctgctaaa tgtaaaattc cagccctgga ccttcttatt
     4501 aagttacttc agacttttag aagttctaga ctcatggatg aatttaaaat tggagaatta
     4561 tttagtaaat tctatggaga acttgcattg aaaaaaaaaa taccagatac agttttagaa
     4621 aaagtatatg agctcctagg attattgggt gaagttcatc ctagtgagat gataaataat
     4681 gcagaaaacc tgttccgcgc ttttctgggt gaacttaaga cccagatgac atcagcagta
     4741 agagagccca aactacctgt tctggcagga tgtctgaagg ggttgtcctc acttctgtgc
     4801 aacttcacta agtccatgga agaagatccc cagacttcaa gggagatttt taattttgta
     4861 ctaaaggcaa ttcgtcctca gattgatctg aagagatatg ctgtgccctc agctggcttg
     4921 cgcctatttg ccctgcatgc atctcagttt agcacctgcc ttctggacaa ctacgtgtct
     4981 ctatttgaag tcttgttaaa gtggtgtgcc cacacaaatg tagaattgaa aaaagctgca
     5041 ctttcagccc tggaatcctt tctgaaacag gtttctaata tggtggcgaa aaatgcagaa
     5101 atgcataaaa ataaactgca gtactttatg gagcagtttt atggaatcat cagaaatgtg
     5161 gattcgaaca acaaggagtt atctattgct atccgtggat atggactttt tgcaggaccg
     5221 tgcaaggtta taaacgcaaa agatgttgac ttcatgtacg ttgagctcat tcagcgctgc
     5281 aagcagatgt tcctcaccca gacagacact ggtgacgacc gtgtttatca gatgccaagc
     5341 ttcctccagt ctgttgcaag cgtcttgctg taccttgaca cagttcctga ggtgtatact
     5401 ccagttctgg agcacctcgt ggtgatgcag atagacagtt tcccacagta cagtccaaaa
     5461 atgcagctgg tgtgttgcag agccatagtg aaggtgttcc tagctttggc agcaaaaggg
     5521 ccagttctca ggaattgcat tagtactgtg gtgcatcagg gtttaatcag aatatgttct
     5581 aaaccagtgg tccttccaaa gggacctgag tctgaatctg aagaccaccg tgcttcaggg
     5641 gaagtcagaa ctggcaaatg gaaggtgccc acatacaaag actacgtgga tctcttcaga
     5701 catctcctga gctctgacca gatgatggat tctattttag cagatgaagc atttttctct
     5761 gtgaattcct ccagtgaaag tctgaatcat ttactttatg atgaatttgt aaaatccgtt
     5821 ttgaagattg ttgagaaatt ggatcttaca cttgaaatac agactgttgg ggaacaagag
     5881 aatggagatg aggcacctgg tgtttggatg atcccaactt cagatccagc ggctaacttg
     5941 catccagcta aacctaaaga tttttcggct ttcattaacc tggtggaatt ttgcagagag
     6001 attctccctg agaaacaagc agaatttttt gaaccatggg tgtactcatt ttcatatgaa
     6061 ttaattttgc aatctacaag gttgcccctc atcagtggtt tctacaaatt gctttctatt
     6121 acagtaagaa atgccaagaa aataaaatat ttcgagggag ttagtccaaa gagtctgaaa
     6181 cactctcctg aagacccaga aaagtattct tgctttgctt tatttgtgaa atttggcaaa
     6241 gaggtggcag ttaaaatgaa gcagtacaaa gatgaacttt tggcctcttg tttgaccttt
     6301 cttctgtcct tgccacacaa catcattgaa ctcgatgtta gagcctacgt tcctgcactg
     6361 cagatggctt tcaaactggg cctgagctat acccccttgg cagaagtagg actgaatgct
     6421 ctagaagaat ggtcaattta tattgacaga catgtaatgc agccttatta caaagacatt
     6481 ctcccctgcc tggatggata cctgaagact tcagccttgt cagatgagac caagaataac
     6541 tgggaagtgt cagctctttc tcgggctgcc cagaaaggat ttaataaagt ggtgttaaag
     6601 catctgaaga agacaaagaa cctttcatca aacgaagcaa tatccttaga agaaataaga
     6661 attagagtag tacaaatgct tggatctcta ggaggacaaa taaacaaaaa tcttctgaca
     6721 gtcacgtcct cagatgagat gatgaagagc tatgtggcct gggacagaga gaagcggctg
     6781 agctttgcag tgccctttag agagatgaaa cctgtcattt tcctggatgt gttcctgcct
     6841 cgagtcacag aattagcgct cacagccagt gacagacaaa ctaaggttgc agcctgtgaa
     6901 cttttacata gcatggttat gtttatgttg ggcaaagcca cgcagatgcc agaaggggga
     6961 cagggagccc cacccatgta ccagctctat aagcggacgt ttcctgtgct gcttcgactt
     7021 gcgtgtgatg ttgatcaggt gacaaggcaa ctgtatgagc cactagttat gcagctgatt
     7081 cactggttca ctaacaacaa gaaatttgaa agtcaggata ctgttgcctt actagaagct
     7141 atattggatg gaattgtgga ccctgttgac agtactttaa gagatttttg tggtcggtgt
     7201 attcgagaat tccttaaatg gtccattaag caaataacac cacagcagca ggagaagagt
     7261 ccagtaaaca ccaaatcgct tttcaagcga ctttatagcc ttgcgcttca ccccaatgct
     7321 ttcaagaggc tgggagcatc acttgccttt aataatatct acagggaatt cagggaagaa
     7381 gagtctctgg tggaacagtt tgtgtttgaa gccttggtga tatacatgga gagtctggcc
     7441 ttagcacatg cagatgagaa gtccttaggt acaattcaac agtgttgtga tgccattgat
     7501 cacctatgcc gcatcattga aaagaagcat gtttctttaa ataaagcaaa gaaacgacgt
     7561 ttgccgcgag gatttccacc ttccgcatca ttgtgtttat tggatctggt caagtggctt
     7621 ttagctcatt gtgggaggcc ccagacagaa tgtcgacaca aatccattga actcttttat
     7681 aaattcgttc ctttattgcc aggcaacaga tcccctaatt tgtggctgaa agatgttctc
     7741 aaggaagaag gtgtctcttt tctcatcaac acctttgagg ggggtggctg tggccagccc
     7801 tcgggcatcc tggcccagcc caccctcttg taccttcggg ggccattcag cctgcaggcc
     7861 acgctatgct ggctggacct gctcctggcc gcgttggagt gctacaacac gttcattggc
     7921 gagagaactg taggagcgct ccaggtccta ggtactgaag cccagtcttc acttttgaaa
     7981 gcagtggctt tcttcttaga aagcattgcc atgcatgaca ttatagcagc agaaaagtgc
     8041 tttggcactg gggcagcagg taacagaaca agcccacaag agggagaaag gtacaactac
     8101 agcaaatgca ccgttgtggt ccggattatg gagtttacca cgactctgct aaacacctcc
     8161 ccggaaggat ggaagctcct gaagaaggac ttgtgtaata cacacctgat gagagtcctg
     8221 gtgcagacgc tgtgtgagcc cgcaagcata ggtttcaaca tcggagacgt ccaggttatg
     8281 gctcatcttc ctgatgtttg tgtgaatctg atgaaagctc taaagatgtc cccatacaaa
     8341 gatatcctag agacccatct gagagagaaa ataacagcac agagcattga ggagctttgt
     8401 gccgtcaact tgtatggccc tgacgcgcaa gtggacagga gcaggctggc tgctgttgtg
     8461 tctgcctgta aacagcttca cagagctggg cttctgcata atatattacc gtctcagtcc
     8521 acagatttgc atcattctgt tggcacagaa cttctttccc tggtttataa aggcattgcc
     8581 cctggagatg agagacagtg tctgccttct ctagacctca gttgtaagca gctggccagc
     8641 ggacttctgg agttagcctt tgcttttgga ggactgtgtg agcgccttgt gagtcttctc
     8701 ctgaacccag cggtgctgtc cacggcgtcc ttgggcagct cacagggcag cgtcatccac
     8761 ttctcccatg gggagtattt ctatagcttg ttctcagaaa cgatcaacac ggaattattg
     8821 aaaaatctgg atcttgctgt attggagctc atgcagtctt cagtggataa taccaaaatg
     8881 gtgagtgccg ttttgaacgg catgttagac cagagcttca gggagcgagc aaaccagaaa
     8941 caccaaggac tgaaacttgc gactacaatt ctgcaacact ggaagaagtg tgattcatgg
     9001 tgggccaaag attcccctct cgaaactaaa atggcagtgc tggccttact ggcaaaaatt
     9061 ttacagattg attcatctgt atcttttaat acaagtcatg gttcattccc tgaagtcttt
     9121 acaacatata ttagtctact tgctgacaca aagctggatc tacatttaaa gggccaagct
     9181 gtcactcttc ttccattctt caccagcctc actggaggca gtctggagga acttagacgt
     9241 gttctggagc agctcatcgt tgctcacttc cccatgcagt ccagggaatt tcctccagga
     9301 actccgcggt tcaataatta tgtggactgc atgaaaaagt ttctagatgc attggaatta
     9361 tctcaaagcc ctatgttgtt ggaattgatg acagaagttc tttgtcggga acagcagcat
     9421 gtcatggaag aattatttca atccagtttc aggaggattg ccagaagggg ttcatgtgtc
     9481 acacaagtag gccttctgga aagcgtgtat gaaatgttca ggaaggatga cccccgccta
     9541 agtttcacac gccagtcctt tgtggaccgc tccctcctca ctctgctgtg gcactgtagc
     9601 ctggatgctt tgagagaatt cttcagcaca attgtggtgg atgccattga tgtgttgaag
     9661 tccaggttta caaagctaaa tgaatctacc tttgatactc aaatcaccaa gaagatgggc
     9721 tactataaga ttctagacgt gatgtattct cgccttccca aagatgatgt tcatgctaag
     9781 gaatcaaaaa ttaatcaagt tttccatggc tcgtgtatta cagaaggaaa tgaacttaca
     9841 aagacattga ttaaattgtg ctacgatgca tttacagaga acatggcagg agagaatcag
     9901 ctgctggaga ggagaagact ttaccattgt gcagcataca actgcgccat atctgtcatc
     9961 tgctgtgtct tcaatgagtt aaaattttac caaggttttc tgtttagtga aaaaccagaa
    10021 aagaacttgc ttatttttga aaatctgatc gacctgaagc gccgctataa ttttcctgta
    10081 gaagttgagg ttcctatgga aagaaagaaa aagtacattg aaattaggaa agaagccaga
    10141 gaagcagcaa atggggattc agatggtcct tcctatatgt cttccctgtc atatttggca
    10201 gacagtaccc tgagtgagga aatgagtcaa tttgatttct caaccggagt tcagagctat
    10261 tcatacagct cccaagaccc tagacctgcc actggtcgtt ttcggagacg ggagcagcgg
    10321 gaccccacgg tgcatgatga tgtgctggag ctggagatgg acgagctcaa tcggcatgag
    10381 tgcatggcgc ccctgacggc cctggtcaag cacatgcaca gaagcctggg cccgcctcaa
    10441 ggagaagagg attcagtgcc aagagatctt ccttcttgga tgaaattcct ccatggcaaa
    10501 ctgggaaatc caatagtacc attaaatatc cgtctcttct tagccaagct tgttattaat
    10561 acagaagagg tctttcgccc ttacgcgaag cactggctta gccccttgct gcagctggct
    10621 gcttctgaaa acaatggagg agaaggaatt cactacatgg tggttgagat agtggccact
    10681 attctttcat ggacaggctt ggccactcca acaggggtcc ctaaagatga agtgttagca
    10741 aatcgattgc ttaatttcct aatgaaacat gtctttcatc caaaaagagc tgtgtttaga
    10801 cacaaccttg aaattataaa gacccttgtc gagtgctgga aggattgttt atccatccct
    10861 tataggttaa tatttgaaaa gttttccggt aaagatccta attctaaaga caactcagta
    10921 gggattcaat tgctaggcat cgtgatggcc aatgacctgc ctccctatga cccacagtgt
    10981 ggcatccaga gtagcgaata cttccaggct ttggtgaata atatgtcctt tgtaagatat
    11041 aaagaagtgt atgccgctgc agcagaagtt ctaggactta tacttcgata tgttatagag
    11101 agaaaaaaca tactggagga gtctctgtgt gaactggttg cgaaacaatt gaagcaacat
    11161 cagaatacta tggaggacaa gtttattgtg tgcttgaaca aagtgaccaa gagcttccct
    11221 cctcttgcag acaggttcat gaatgctgtg ttctttctgc tgccaaaatt tcatggagtg
    11281 ttgaaaacac tctgtctgga ggtggtactt tgtcgtgtgg agggaatgac agagctgtac
    11341 ttccagttaa agagcaagga cttcgttcaa gtcatgagac atagagatga tgaaagacaa
    11401 aaagtatgtt tggacataat ttataagatg atgccaaagt taaaaccagt agaactccga
    11461 gaacttctga accccgttgt ggaattcgtt tcccatcctt ctacaacatg tagggaacaa
    11521 atgtataata ttctcatgtg gattcatgat aattacagag atccagaaag tgagacagat
    11581 aatgactccc aggaaatatt taagttggca aaagatgtgc tgattcaagg attgatcgat
    11641 gagaaccctg gacttcaatt aattattcga aatttctgga gccatgaaac taggttacct
    11701 tcaaatacct tggaccggtt gctggcacta aattccttat attctcctaa gatagaagtg
    11761 cactttttaa gtttagcaac aaattttctg ctcgaaatga ccagcatgag cccagattat
    11821 ccaaacccca tgttcgagca tcctctgtca gaatgcgaat ttcaggaata taccattgat
    11881 tctgattggc gtttccgaag tactgttctc actccgatgt ttgtggagac ccaggcctcc
    11941 cagggcactc tccaggcccg tacccaggaa gggtccctct cagctcgctg gccagtggca
    12001 gggcagataa gggccaccca gcagcagcat gacttcacac tgacacagac tgcagatgga
    12061 agaagctcat ttgattggct gaccgggagc agcactgacc cgctggtcga ccacaccagt
    12121 ccctcatctg actccttgct gtttgcccac aagaggagtg aaaggttaca gagagcaccc
    12181 ttgaagtcag tggggcctga ttttgggaaa aaaaggctgg gccttccagg ggacgaggtg
    12241 gataacaaag tgaaaggtgc ggccggccgg acggacctac tacgactgcg cagacggttt
    12301 atgagggacc aggagaagct tagtttgatg tatgcccgaa aaggcgttgc tgagcaaaaa
    12361 cgagagaagg aaatcaagag tgagttaaaa atgaagcagg atgcccaggt cgttctgtac
    12421 agaagctacc ggcacggaga ccttcctgac attcagatca agcacagcag cctcatcacc
    12481 ccgttacagg ccgtggccca gagggaccca ataatcgcga aacagctctt tagcagcttg
    12541 ttttctggaa ttttgaaaga gatggataaa tttaagacac tgtctgaaaa aaacaacatc
    12601 actcaaaagt tgcttcaaga cttcaatcgt tttcttaata ccaccttctc tttctttcca
    12661 ccctttgtct cttgtattca ggacattagc tgtcagcacg cagccctgct gagcctcgac
    12721 ccagcggctg ttagcgctgg ttgcctggcc agcctacagc agcctgttgg catccgccta
    12781 ctagaggagg ctattctttg tctgctgcct gctgagctgc ccgccaagcg agtccgtggg
    12841 aaggctagac ctcctcctga tgtcctcaga tgggtggagc ttgctaagct gtatagatca
    12901 attggagaat acgacgtcct ccgtgggatt tttaccagtg agataggaac aaagcaaatc
    12961 actcagagtg cattattagc agaagccaga agtgattatt ctgaagctgc taagcagtat
    13021 gatgaggctc tcaataaaca agactgggta gatggtgagc ccacagaagc cgagaaggat
    13081 ttttgggaac ttgcatccct tgactgttac aaccaccttg ctgagtggaa atcacttgaa
    13141 tactgttcta cagccagtat agacagtgag aaccccccag acctaaataa aatctggagt
    13201 gaaccatttt atcaggaaac atatctacct tacatgatcc gcagcaagct gaagctgctg
    13261 ctccagggag aggctgacca gtccctgctg acatttattg acaaagctat gcacggggag
    13321 ctccagaagg cgattctaga gcttcattac agtcaagagc tgagtctgct ttacctcctg
    13381 caagatgatg ttgacagagc caaatattac attcaaaatg gcattcagag ttttatgcag
    13441 aattattcta gtattgatgt cctcttacac caaagtagac tcaccaaatt gcagtctgta
    13501 caggctttaa cagaaattca ggagttcatc agctttataa gcaaacaagg caatttatca
    13561 tctcaagttc cccttaagag acttctgaac acctgggcaa acagatatcc agatgctaaa
    13621 atggacccaa tgaacatctg ggatgacatc atcacaaatc gatgtttctt tctcagcaaa
    13681 atagaggaga agcttacccc tcttccagaa gataatagta tgaatgtgga tcaagatgga
    13741 gaccccagtg acaggatgga agtgcaagag caggaagaag atatcagctc cctgatcagg
    13801 agttgcaagt tttccatgaa aatgaagatg atagacagtg cccggaagca gaacaatttc
    13861 tcacttgcta tgaaactact gaaggagctg cataaagagt caaaaaccag agacgattgg
    13921 ctggtgagct gggtgcagag ctactgccgc ctgagccact gccggagccg gtcccagggc
    13981 tgctctgagc aggtgctcac tgtgctgaaa acagtctctt tgttggatga gaacaacgtg
    14041 tcaagctact taagcaaaaa tattctggct ttccgtgacc agaacattct cttgggtaca
    14101 acttacagga tcatagcgaa tgctctcagc agtgagccag cctgccttgc tgaaatcgag
    14161 gaggacaagg ctagaagaat cttagagctt tctggatcca gttcagagga ttcagagaag
    14221 gtgatcgcgg gtctgtacca gagagcattc cagcacctct ctgaggctgt gcaggcggct
    14281 gaggaggagg cccagcctcc ctcctggagc tgtgggcctg cagctggggt gattgatgct
    14341 tacatgacgc tggcagattt ctgtgaccaa cagctgcgca aggaggaaga gaatgcatca
    14401 gttactgatt ctgcagaact gcaggcgtat ccagcacttg tggtggagaa aatgttgaaa
    14461 gctttaaaat taaattccaa tgaagccaga ttgaagtttc ctagattact tcagattata
    14521 gaacggtatc cagaggagac tttgagcctc atgacaaaag agatctcttc cgttccctgc
    14581 tggcagttca tcagctggat cagccacatg gtggccttac tggacaaaga ccaagccgtt
    14641 gctgttcagc actctgtgga agaaatcact gataactacc cgcaggctat tgtttatccc
    14701 ttcatcataa gcagcgaaag ctattccttc aaggatactt ctactggtca taagaataag
    14761 gagtttgtgg caaggattaa aagtaagttg gatcaaggag gagtgattca agattttatt
    14821 aatgccttag atcagctctc taatcctgaa ctgctcttta aggattggag caatgatgta
    14881 agagctgaac tagcaaaaac ccctgtaaat aaaaaaaaca ttgaaaaaat gtatgaaaga
    14941 atgtatgcag ccttgggtga cccaaaggct ccaggcctgg gggcctttag aaggaagttt
    15001 attcagactt ttggaaaaga atttgataaa cattttggga aaggaggttc taaactactg
    15061 agaatgaagc tcagtgactt caacgacatt accaacatgc tacttttaaa aatgaacaaa
    15121 gactcaaagc cccctgggaa tctgaaagaa tgttcaccct ggatgagcga cttcaaagtg
    15181 gagttcctga gaaatgagct ggagattccc ggtcagtatg acggtagggg aaagccattg
    15241 ccagagtacc acgtgcgaat cgccgggttt gatgagcggg tgacagtcat ggcgtctctg
    15301 cgaaggccca agcgcatcat catccgtggc catgacgaga gggaacaccc tttcctggtg
    15361 aagggtggcg aggacctgcg gcaggaccag cgcgtggagc agctcttcca ggtcatgaat
    15421 gggatcctgg cccaagactc cgcctgcagc cagagggccc tgcagctgag gacctatagc
    15481 gttgtgccca tgacctccag gttaggatta attgagtggc ttgaaaatac tgttaccttg
    15541 aaggaccttc ttttgaacac catgtcccaa gaggagaagg cggcttacct gagtgatccc
    15601 agggcaccgc cgtgtgaata taaagattgg ctgacaaaaa tgtcaggaaa acatgatgtt
    15661 ggagcttaca tgctaatgta taagggcgct aatcgtactg aaacagtcac gtcttttaga
    15721 aaacgagaaa gtaaagtgcc tgctgatctc ttaaagcggg ccttcgtgag gatgagtaca
    15781 agccctgagg ctttcctggc gctccgctcc cacttcgcca gctctcacgc tctgatatgc
    15841 atcagccact ggatcctcgg gattggagac agacatctga acaactttat ggtggccatg
    15901 gagactggtg gcgtgatcgg gatcgacttt gggcatgcgt ttggatccgc tacacagttt
    15961 ctgccagtcc ctgagttgat gccttttcgg ctaactcgcc agtttatcaa tctgatgtta
    16021 ccaatgaaag aaacgggcct tatgtacagc atcatggtac acgcactccg ggccttccgc
    16081 tcagaccctg gcctgctcac caacaccatg gatgtgtttg tcaaggagcc ctcctttgat
    16141 tggaaaaatt ttgaacagaa aatgctgaaa aaaggagggt catggattca agaaataaat
    16201 gttgctgaaa aaaattggta cccccgacag aaaatatgtt acgctaagag aaagttagca
    16261 ggtgccaatc cagcagtcat tacttgtgat gagctactcc tgggtcatga gaaggcccct
    16321 gccttcagag actatgtggc tgtggcacga ggaagcaaag atcacaacat tcgtgcccaa
    16381 gaaccagaga gtgggctttc agaagagact caagtgaagt gcctgatgga ccaggcaaca
    16441 gaccccaaca tccttggcag aacctgggaa ggatgggagc cctggatgtg aggtctgcgg
    16501 ccgcgatctg tcgacgataa acccgctgat cagcctcgac tgtgccttct agttgccagc
    16561 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
    16621 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
    16681 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
    16741 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagatcg gatccatcga
    16801 tataacttcg tataggagac tttatacgaa gttatctcga tgaaggggtt ctctagatcg
    16861 agaattcact ggccgtcgtt ttacaaggcg cgcccccggg taccgagctc gaattcactg
    16921 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
//
