LOCUS       sequence-47057-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-VRK2
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..120
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             497..508
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggggggaatc agcctggatg gcaataatga ttttattttg actgatagtg acctgttcgt
       61 tgcaacaaat tgataagcaa tgctttctta taatgccaac tttgtacaag aaagttggca
      121 agagaaaaaa taaagcaaga aatactaaca tcaaaaggac aggtatgatg atgcgataat
      181 aataaacatc tgcgtttgtc tcttcactaa gagtaaactg ggaaattgta ggccaaagtc
      241 cagttgaact ttctaagtct gtgatccccg tgctgactgt ggaagtgtat ttataccaag
      301 atggagatct tgacttcttg aatatatctg gactggtaaa atcttgatga ggctcataaa
      361 atgagtttgg gaattgtgta tagctgattt tttgtgggaa actgtttact tcattcaaag
      421 gttcttgaga ctcttgatat ttctgtcttc tccttgtgct ttcctgagct gcttcattgt
      481 tcatcaatcc aatcagtttc tcctctttct gcactttcca tgttgcacag gactcagcgc
      541 ttctctcact gtggactttt ttttcgatta acctattgtg tgccttgttg acttgctttg
      601 ttgcagcctt ttgtgaatca actttttgac tgtttggagt atggacattt atactctgtc
      661 cttttgtgga aaagtccagt ggtcctaaag gtattccatg agggttcaaa attttcttga
      721 gggcttgata gtttggcttt tcatcatatg ctaaactatg agcacatacc aaaaattggg
      781 ctatttcaca g
//
