LOCUS       sequence-470592-        7353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express IFIH1-HiBiT in Mammalian Cells under a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: IFIH1-HiBiT Fusion Vector
FEATURES             Location/Qualifiers
     source          1..7353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4296..4314)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4351..4472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4362..4381)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4416..4435
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4717..5074
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4717..4908
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4740..4760)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4925..5060
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5089..5136
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5168..5962
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5222..5241)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5832..5851
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6026..6074
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(6313..6901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6393..6412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    7017..7300
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgtcg aatgggtatt
     1081 ccacagacga gaatttccgc tatctcatct cgtgcttcag ggccagggtg aaaatgtaca
     1141 tccaggtgga gcctgtgctg gactacctga cctttctgcc tgcagaggtg aaggagcaga
     1201 ttcagaggac agtcgccacc tccgggaaca tgcaggcagt tgaactgctg ctgagcacct
     1261 tggagaaggg agtctggcac cttggttgga ctcgggaatt cgtggaggcc ctccggagaa
     1321 ccggcagccc tctggccgcc cgctacatga accctgagct cacggacttg ccctctccat
     1381 cgtttgagaa cgctcatgat gaatatctcc aactgctgaa cctccttcag cccactctgg
     1441 tggacaagct tctagttaga gacgtcttgg ataagtgcat ggaggaggaa ctgttgacaa
     1501 ttgaagacag aaaccggatt gctgctgcag aaaacaatgg aaatgaatca ggtgtaagag
     1561 agctactaaa aaggattgtg cagaaagaaa actggttctc tgcatttctg aatgttcttc
     1621 gtcaaacagg aaacaatgaa cttgtccaag agttaacagg ctctgattgc tcagaaagca
     1681 atgcagagat tgagaattta tcacaagttg atggtcctca agtggaagag caacttcttt
     1741 caaccacagt tcagccaaat ctggagaagg aggtctgggg catggagaat aactcatcag
     1801 aatcatcttt tgcagattct tctgtagttt cagaatcaga cacaagtttg gcagaaggaa
     1861 gtgtcagctg cttagatgaa agtcttggac ataacagcaa catgggcagt gattcaggca
     1921 ccatgggaag tgattcagat gaagagaatg tggcagcaag agcatccccg gagccagaac
     1981 tccagctcag gccttaccaa atggaagttg cccagccagc cttggaaggg aagaatatca
     2041 tcatctgcct ccctacaggg agtggaaaaa ccagagtggc tgtttacatt gccaaggatc
     2101 acttagacaa gaagaaaaaa gcatctgagc ctggaaaagt tatagttctt gtcaataagg
     2161 tactgctagt tgaacagctc ttccgcaagg agttccaacc atttttgaag aaatggtatc
     2221 gtgttattgg attaagtggt gatacccaac tgaaaatatc atttccagaa gttgtcaagt
     2281 cctgtgatat tattatcagt acagctcaaa tccttgaaaa ctccctctta aacttggaaa
     2341 atggagaaga tgctggtgtt caattgtcag acttttccct cattatcatt gatgaatgtc
     2401 atcacaccaa caaagaagca gtgtataata acatcatgag gcattatttg atgcagaagt
     2461 tgaaaaacaa tagactcaag aaagaaaaca aaccagtgat tccccttcct cagatactgg
     2521 gactaacagc ttcacctggt gttggagggg ccacgaagca agccaaagct gaagaacaca
     2581 ttttaaaact atgtgccaat cttgatgcat ttactattaa aactgttaaa gaaaaccttg
     2641 atcaactgaa aaaccaaata caggagccat gcaagaagtt tgccattgca gatgcaacca
     2701 gagaagatcc atttaaagag aaacttctag aaataatgac aaggattcaa acttattgtc
     2761 aaatgagtcc aatgtcagat tttggaactc aaccctatga acaatgggcc attcaaatgg
     2821 aaaaaaaagc tgcaaaagaa ggaaatcgca aagaacgtgt ttgtgcagaa catttgagga
     2881 agtacaatga ggccctacaa attaatgaca caattcgaat gatagatgcg tatactcatc
     2941 ttgaaacttt ctataatgaa gagaaagata agaagtttgc agtcatagaa gatgatagtg
     3001 atgagggtgg tgatgatgag tattgtgatg gtgatgaaga tgaggatgat ttaaagaaac
     3061 ctttgaaact ggatgaaaca gatagatttc tcatgacttt attttttgaa aacaataaaa
     3121 tgttgaaaag gctggctgaa aacccagaat atgaaaatga aaagctgacc aaattaagaa
     3181 ataccataat ggagcaatat actaggactg aggaatcagc acgaggaata atctttacaa
     3241 aaacacgaca gagtgcatat gcgctttccc agtggattac tgaaaatgaa aaatttgctg
     3301 aagtaggagt caaagcccac catctgattg gagctggaca cagcagtgag ttcaaaccca
     3361 tgacacagaa tgaacaaaaa gaagtcatta gtaaatttcg cactggaaaa ataaatctgc
     3421 ttatcgctac cacagtggca gaagaaggtc tggatattaa agaatgtaac attgttatcc
     3481 gttatggtct cgtcaccaat gaaatagcca tggtccaggc ccgtggtcga gccagagctg
     3541 atgagagcac ctacgtcctg gttgctcaca gtggttcagg agttatcgaa catgagacag
     3601 ttaatgattt ccgagagaag atgatgtata aagctataca ttgtgttcaa aatatgaaac
     3661 cagaggagta tgctcataag attttggaat tacagatgca aagtataatg gaaaagaaaa
     3721 tgaaaaccaa gagaaatatt gccaagcatt acaagaataa cccatcacta ataactttcc
     3781 tttgcaaaaa ctgcagtgtg ctagcctgtt ctggggaaga tatccatgta attgagaaaa
     3841 tgcatcacgt caatatgacc ccagaattca aggaacttta cattgtaaga gaaaacaaag
     3901 cactgcaaaa gaagtgtgcc gactatcaaa taaatggtga aatcatctgc aaatgtggcc
     3961 aggcttgggg aacaatgatg gtgcacaaag gcttagattt gccttgtctc aaaataagga
     4021 attttgtagt ggttttcaaa aataattcaa caaagaaaca atacaaaaag tgggtagaat
     4081 tacctatcac atttcccaat cttgactatt cagaatgctg tttatttagt gatgaggatg
     4141 tttctcaagg cagttcaggt ggctcgagcg gtgtgagcgg ctggcggctg ttcaagaaga
     4201 ttagctaagg ccgcgactct agagtcgacc tgcaggcatg caagctgatc cggctgctaa
     4261 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
     4321 ccttggggcg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     4381 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     4441 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     4501 ttcaggttca gggggagatg tgggaggttt ttttaagcaa gtaaaacctc tacaaatgtg
     4561 gtaaaatcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
     4621 ttacgcatct gtgcggtatt tcacaccgca tacgcggatc tgcgcagcac catggcctga
     4681 aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg
     4741 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4801 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4861 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4921 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4981 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     5041 aggaggcttt tttggaggcc taggcttttg caaaaagctt aattaactgt tgacaattaa
     5101 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aacccaggag
     5161 gcagatcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5221 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5281 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5341 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5401 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5461 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5521 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5581 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5641 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     5701 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5761 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5821 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5881 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5941 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     6001 cccaacctgc catcacgatg gccgcaataa aatatcttta ttttcattac atctgtgtgt
     6061 tggttttttg tgtgaatcga tagcgataag gatcctcttt gcgcttgcgt tttcccttgt
     6121 ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt
     6181 ctacccggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6241 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6301 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6361 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6421 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6481 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6541 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6601 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6661 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6721 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     6781 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6841 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatttca
     6901 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6961 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tatagtccgg
     7021 aaatacagga acgcacgctg gatggccctt cgctgggatg gtgaaaccat gaaaaatggc
     7081 agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc tctggttgca taggtattca
     7141 tacggttaaa atttatcagg cgcgattgcg gcagtttttc gggtggtttg ttgccatttt
     7201 tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc ggtgcgtaca attaagggat
     7261 tatggtaaat ccacttactg tctgccctcg tagccatcga gataaaccgc agtactccgg
     7321 ccacgatgcg tccggcgtag aggatcgaga tct
//
