LOCUS       sequence-47068-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-LOC91807
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    57..156
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tggaaacagc tatgaccatg taatacgact cactataggg gatatcagct ggatggcaaa
       61 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
      121 ttcttataat gccaactttg tacaagaaag ttggcaaggg agaagttgga aatttcctta
      181 acctgttggc agcagtcacc acatagaaat gtttcttcca ttttctttga gctatgtatt
      241 tctgcagcag tagttgggat ttgagacgag ttttggatct tgaagctttg gcaggcaaat
      301 tattcagcca ctcgtgtttc aggcactgtg tggcactcat tctgcagctc ttctctttga
      361 ccagcaaccg ggaaacaaag tccttggcct cctccgagag cccttcaaag gtgtcagcat
      421 caaaatccca gctacagttt acaatgaaat tcatggtctc tgcatctgtt tcccctagaa
      481 atggggacaa gccactgagt agcatgtagg tgatgactcc cacactccac atgtctgtgg
      541 ggaatgagac aaactcataa ttgacgactt ctggggccag gaactcagga gtgccgaagt
      601 tcaccttcag cttctctcga ggcttgtacc ttctggccag cccaaagtca atgatcttaa
      661 tttgatgtcc tgtctgattg acgcacaata tgttctccgg cttgaggtcc aggtgcagga
      721 tgtagtgctg gtgca
//
