LOCUS       sequence-47069-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PRKAG3
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    57..156
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gggaaacagc tatgaccatg taatacgact cactataggg gatatcagct ggatggcaaa
       61 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
      121 ttcttataat gccaactttg tacaagaaag ttggcaaggc cccgagggca tcgatgccag
      181 cagggctgag caccagtgcc tgaaggatgt cggagaggga gaccacgccc aagagatgct
      241 gggtctcgtc cactagcacc agcctgtgta cctgctcccg agcaatcctg tcgatcactt
      301 cccccaagct ctcgtggggc tggcaggaaa ggactccctc cagacatagt gtcctctgcc
      361 tcagggcttc tcccacactc atgtccaggt ggttgtaggt ttgctgggca gccaggtgaa
      421 tcacatcaaa gcgggaatag aggcccacga cctgaccaca ttcgttgacc acaggcagtg
      481 cagacacacg ccggtccaca aagatgtcca gtgcagtcag gatgggtgct gtctccagca
      541 ccacagccaa gtctcggaat gtgccgatgc ccaaatcttg gatagtgcgg tagaggaagg
      601 agggccgggg cagcagggaa ccaaagatgt gcaggaactt gagcaggcgt ttgtgtgtga
      661 ggatgtggag tacgttgcct gacaccgggt caagaacagg caggcgatgg atccggttct
      721 tgatgagggt gtagacagct tcaaacaggc tatcattagg agagatggag accagaagct
      781 t
//
