LOCUS       sequence-47070-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PRKCA
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    56..155
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gaaaacagct atgaccatgt aatacgactc actatagggg atatcagctg gatggcaaat
       61 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt
      121 tcttataatg ccaactttgt acaagaaagt tggcaatact gcactctgta agatggggtg
      181 cacaaactgg gggttgacat acgagaaccc ttcaaaatca gactggtcta tgttagcaat
      241 aaccagctga tcaggtggtg ttaagacggg ctgtcctcgt gtgaagaact tgtcaaagtt
      301 ctctgctcct ttgccacaca ctttgggctt gaatggtggc tggatctccc tgttctccag
      361 tttttcccag tcgatcctcc ggaagaaggc atgctctctc acgtccctct ccccctcagg
      421 cccacagccc agccgcttgg ctgggtgttt ggtcatcagt cctttgcaga tagaaacagc
      481 ctccttggac aaggattttg gataggaaac gttgtgctcc atgatagact gaaatagctc
      541 gtcttcatct tcaccatcaa atggaggctg cccggcaagc atttcataca acaggacgcc
      601 ataggcccac cagtccacag attttccata cggctgataa gcgattatct ctggggcgat
      661 ataatctgga gtcccacaga aggtcctggt cgtgactcca tccatcatgt gttccttgca
      721 catcccaaag tcagcaattt tgatatgtcc ttctgaatcc aacatgacgt tatctaactt
      781 cagatcccta t
//
