LOCUS       sequence-471001-       10184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of Hspa1l-mRuby2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Suk TR, Part CE, Zhang JL, Nguyen TT, Heer MM, Caballero-Gomez A, 
            Grybas VS, McKeever PM, Nguyen B, Ali T, Callaghan SM, Woulfe JM, 
            Robertson J, Rousseaux MWC
  TITLE     A stress-dependent TDP-43 SUMOylation program preserves neuronal 
            function.
  JOURNAL   Mol Neurodegener. 2025 Mar 28;20(1):38. doi: 
            10.1186/s13024-025-00826-z.
  PUBMED    40149017
REFERENCE   2  (bases 1 to 10184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Neurodegener."; date: "2025-03-28"; volume: "20(1):38. doi"; pages: 
            " 10.1186/s13024-025-00826-z"
COMMENT     Sequence Label: pLV-Hspa1l-mRuby2
FEATURES             Location/Qualifiers
     source          1..10184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             305..904
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(305..324)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     801..821
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    929..1518
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(983..1003)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1401..1412)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1605..1838
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     rep_origin      2099..2234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2352..2940)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2432..2451)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(3302..3370,3371..4162)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3520..3539)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      4175..4311
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     enhancer        4388..4691
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4692..4895
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4846..4866
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4910..5090
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5134..5259
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5752..5985
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6170..6214
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6481..6598
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6694..7095
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             9145..9855
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     misc_feature    join(9935..10184,1..302)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10079..10096)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
       61 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
      121 atggctcccc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
      181 tatgggatct gatctggggc ctcggtgcac atgcttttca tgtgtttagt cgaggttaaa
      241 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
      301 taacatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
      361 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
      421 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
      481 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
      541 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
      601 cggttcccgg ctggccgcgc agcaacagat ggagggcctc ctggcgccgc accggcccaa
      661 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
      721 gggcagcgcc gtcgtgctcc ccggagtgga ggccgccgag cgcgccgggg tgcccgcctt
      781 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
      841 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
      901 ctaggctagc ttgactgact gagtcgacaa tcaacctttt ggattacaaa atttgtgaaa
      961 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     1021 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     1081 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     1141 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     1201 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     1261 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     1321 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     1381 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     1441 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     1501 tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa
     1561 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     1621 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     1681 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1741 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1801 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt cctggccaac gtgagcaccg
     1861 tgctgacctc caaatatcgt taagctggag cctgggagcc ggcctggccc tccgcccccc
     1921 ccacccccgc agcccacccc tggtctttga ataaagtctg agtgagtggc cgacagtgcc
     1981 cgtggagttc tcgtgacctg aggtgcaggg ccggcgctag ggacacgtcc gtgcacgtgc
     2041 cgaggccccc tgtgcagctg caagggacag gcctagccct gcaggcctaa ctccgcccat
     2101 cccgccccta actccgccca gttccgccca ttctccgcct catggctgac taattttttt
     2161 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggacg
     2221 cttttttgga ggccgaggct tttgcaaaga tcgaacaaga gacaggacct gcaggttaat
     2281 taaatttaaa tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2341 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2401 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2461 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2521 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2581 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2641 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2701 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2761 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2821 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2881 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2941 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3001 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3061 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3121 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcattt
     3181 aaatggccgg cctggcgcgc cgtttaaacc tagatattga tagtctgatc ggtcaacgta
     3241 taatcgagtc ctagcttttg caaacatcta tcaagagaca ggatcagcag gaggctttcg
     3301 catgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3361 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3421 gcgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3481 cgaagaacgc tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3541 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3601 ggttgagtat tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3661 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3721 tggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3781 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3841 gcctgtagca atggcaacaa ccttgcgtaa actattaact ggcgaactac ttactctagc
     3901 ttcccggcaa cagttgatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3961 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4021 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4081 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4141 ctcactgatt aagcattggt aaccgattct aggtgcattg gcgcagaaaa aaatgcctga
     4201 tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga tacggcttcc
     4261 ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa tttatcctta agatcccgaa
     4321 tcgtttaaac gcgatcgcag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4381 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4441 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4501 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4561 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4621 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4681 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     4741 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     4801 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     4861 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgg ggtctctctg
     4921 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4981 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5041 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     5101 aacagggacc tgaaagcgaa agggaaacca gagctctctc gacgcaggac tcggcttgct
     5161 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     5221 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     5281 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     5341 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     5401 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     5461 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     5521 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     5581 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     5641 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     5701 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     5761 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcctcaa tgacgctgac
     5821 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     5881 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     5941 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     6001 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     6061 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     6121 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     6181 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     6241 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     6301 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     6361 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     6421 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac
     6481 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     6541 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttgg
     6601 ctcccgatcg ttgcgttaca cacacaatta ctgctgatcg agtgtagcct tcgaatgaaa
     6661 gaccccacct gtaggtttgg caagatagct gcagtaacgc cattttgcaa ggcatggaaa
     6721 aataccaaac caagaataga gaagttcaga tcaagggcgg gtacatgaaa atagctaacg
     6781 ttgggccaaa caggatatct gcggtgagca gtttcggccc cggcccgggg ccaagaacag
     6841 atggtcaccg cagtttcggc cccggcccga ggccaagaac agatggtccc cagatatggc
     6901 ccaaccctca gcagtttctt aagacccatc agatgtttcc aggctccccc aaggacctga
     6961 aatgaccctg cgccttattt gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg
     7021 cttctgcttc ccgagctcta taaaagagct cacaacccct cactcggcgc gccagtcctc
     7081 cgattgactg agtcgccctg atcattgtcg atcctaccat ccactcgaca cacccgccag
     7141 ggcccgcatc caccatcgca gacttatcat ggatccatgg ccgcaaataa gggtatggca
     7201 atcggcatag atttgggcac aacttatagc tgcgttggcg tgtttcagca cgggaaagtc
     7261 gagataattg ctaacgacca aggaaacaga accaccccta gctatgtggc ctttactgac
     7321 acagagcgct tgattggcga cgccgccaaa aatcaagtag caatgaaccc ccagaacaca
     7381 gtcttcgacg caaagaggtt gataggacga aagttcaatg atcctgtagt gcagagcgac
     7441 atgaagctct ggcccttcca ggtgattaac gaagccggga agccaaaggt gatggtttcc
     7501 tataaggggg agaaaaaggc attctatccc gaggagatct caagtatggt attgaccaaa
     7561 atgaaggaga ccgctgaagc atttttgggc cataacgtta ctaatgctgt tatcaccgtc
     7621 cccgcatact ttaacgactc acaacggcaa gccacaaaag atgccggagt aattgcaggg
     7681 ctcaacgttc ttcgcatcat aaacgagcct accgcagccg caatcgccta tgggcttgac
     7741 aaaggatcac atggtgagag acatgtactc atattcgact tgggtggcgg aacattcgac
     7801 gtcagtattt tgacaatcga cgatgggata ttcgaggtta aagctaccgc tggtgataca
     7861 catctgggag gggaggactt tgataataga cttgtctctc attttgtcga agagttcaaa
     7921 agaaagcaca agaaggatat ttctcagaat aaacgggctg ttaggaggct ccgcacagct
     7981 tgtgaacgag ctaaacgaac tctttcatcc tccacccaag ccaacttgga gattgatagt
     8041 ttgtacgagg gcatcgactt ttatacatct ataaccagag caagattcga ggagctgtgc
     8101 gcagatttgt ttcgcgggac tctcgagcca gtggaaaaga gtcttcggga cgcaaagatg
     8161 gacaaggcta agatccacga catcgtgctt gtcgggggat ctactagaat tcccaaggtt
     8221 caaaagctct tgcaggacta tttcaatggg agagacctga ataaaagtat caaccccgat
     8281 gaagcagtcg cttatggagc tgctgtacaa gccgcaattt tgatgggtga taaatcagag
     8341 aaggttcagg acttgctttt gttggatgtc gccccactga gtctgggtct tgaaaccgcc
     8401 gggggagtta tgacagtctt gatcaagcga aatagcacaa ttcctactaa acagactcaa
     8461 atatttacca catatagcga taatcaacca ggcgtgctta tacaagtgta cgaaggtgag
     8521 agggctatga cacgggataa caatctcctg ggccgatttg acctgacagg catcccccct
     8581 gctccacgcg gcgtacccca gatagaagtc acttttgaca ttgacgctaa cggaatcctt
     8641 aatgtaacag ctatggacaa gtctacaggt aaagccaata aaataacaat aacaaatgat
     8701 aaagggcgac tgtctaaaga agagatcgaa agaatggtgc aagaagctga gaggtataaa
     8761 gccgaagacg aagggcaaag agagaaaata gcagccaaaa acgcactgga gtcctatgcc
     8821 ttcaacatga aaagtgcagt gggtgacgag ggcctcaaag ataaaatcag tgaatctgat
     8881 aagaagaaaa tcctggacaa gtgtaatgaa gttctttcct ggctcgaggc caaccagttg
     8941 gctgagaagg atgaattcga ccataaacgg aaggagcttg aaaatatgtg taacccaatt
     9001 attaccaagt tgtatcagag cggctgcaca ggccctactt gtacacctgg ttatacccca
     9061 ggtcgggctg ccaccggacc tacaatcgaa gaagtagatg gcggaggggg ctctggagga
     9121 ggcggctctg gcggaggagg ctctatggtg tctaagggcg aagagctgat caaggaaaat
     9181 atgcgtatga aggtggtcat ggaaggttcg gtcaacggcc accaattcaa atgcacaggt
     9241 gaaggagaag gcaatccgta catgggaact caaaccatga ggatcaaagt catcgaggga
     9301 ggacccctgc catttgcctt tgacattctt gccacgtcgt tcatgtatgg cagccgtact
     9361 tttatcaagt acccgaaagg cattcctgat ttctttaaac agtcctttcc tgagggtttt
     9421 acttgggaaa gagttacgag atacgaagat ggtggagtcg tcaccgtcat gcaggacacc
     9481 agccttgagg atggctgtct cgtttaccac gtccaagtca gaggggtaaa ctttccctcc
     9541 aatggtcccg tgatgcagaa gaagaccaag ggttgggagc ctaatacaga gatgatgtat
     9601 ccagcagatg gtggtctgag gggatacact catatggcac tgaaagttga tggtggtggc
     9661 catctgtctt gctctttcgt aacaacttac aggtcaaaaa agaccgtcgg gaacatcaag
     9721 atgcccggta tccatgccgt tgatcaccgc ctggaaaggt tagaggaaag tgacaatgaa
     9781 atgttcgtag tacaacgcga acacgcagtt gccaagttcg ccgggcttgg tggtgggatg
     9841 gacgagctgt acaagtagct cgagcttatt ccagatgcgt gcggatggaa ttcgagctcg
     9901 gtaccatgcc aaaagcaaag cgctatcgcg ccttacgtta ctggccgaag ccgcttggaa
     9961 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
    10021 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
    10081 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
    10141 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcg
//
