LOCUS       sequence-471003-        9552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HA-SUMO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Suk TR, Part CE, Zhang JL, Nguyen TT, Heer MM, Caballero-Gomez A, 
            Grybas VS, McKeever PM, Nguyen B, Ali T, Callaghan SM, Woulfe JM, 
            Robertson J, Rousseaux MWC
  TITLE     A stress-dependent TDP-43 SUMOylation program preserves neuronal 
            function.
  JOURNAL   Mol Neurodegener. 2025 Mar 28;20(1):38. doi: 
            10.1186/s13024-025-00826-z.
  PUBMED    40149017
REFERENCE   2  (bases 1 to 9552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Neurodegener."; date: "2025-03-28"; volume: "20(1):38. doi"; pages: 
            " 10.1186/s13024-025-00826-z"
COMMENT     Sequence Label: pL302-HA-SUMO2
FEATURES             Location/Qualifiers
     source          1..9552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..41
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             67..247
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     752..768
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     753..769
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    772..1360
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             878..889
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1287..1307
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1428..1703)
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 2"
                     /label=SUMO2
                     /translation="ADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     CDS             complement(1701..1727)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(1771..2982)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(1915..1934)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    3047..3164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3462..3506)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3691..3924
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4417..4542
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4589..4769
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4787..4985)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4812..4832)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4987..5366
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5538..5557
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5632..5736
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5737..5805,5806..6597)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5955..5974)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6768..7356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7257..7276
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7510..7527
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7644..7665
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7680..7685,7686..7703,7704..7710)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7718..7734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7723..7745
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7742..7758
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7742..7758
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7807..7928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7818..7837)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7872..7891
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8058..8432)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(8451..8498)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(8546..8875)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8560..8695
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8614..8633)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8860..8880
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(8889..9317)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8999..9020)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9211..9230
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    join(9363..9552,1..35)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcaaacaaca gatggctggc aactagaagg cacagtcgag gctgatcagc gggtttaaac
       61 gggccctgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
      121 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
      181 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
      241 gagacccagt acagtccgga tgcagctctc gggccatgtg atgaaatgct aggcggctgt
      301 caaacctcca ctctaatact tctctctccg ggtcatccat cccatgcagg ctcacagggt
      361 gtaacaagcg ggtgttctct ccttcattgg cttcttctac cttctcttgc tcaactggta
      421 ctagcttgta gcaccatcca aaggtcagtg gatatctgat ccctggccct ggtgtgtagt
      481 tctgccaatc agggaagtag ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc
      541 ttcgttggga gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat
      601 ctacagctgc cttgtaagtc attggtctta aaggtacctg aggtgtgact ggaaaaccca
      661 cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct gctgtattgc
      721 tacttgtgat tgctccatgt ttttctaggt ctcgaggtcg acggtatcga tgcggggagg
      781 cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca
      841 gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc
      901 agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa ggaaaggacg
      961 atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc
     1021 agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag
     1081 tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa
     1141 acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca
     1201 gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat
     1261 acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac
     1321 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga
     1381 atcgataagc ttggctgcag gtcgactcta gatgcatgct cgagtcaacc tcccgtctgc
     1441 tgttggaaca catcaattgt atcttcatcc tccatttcca actgtgcagg tgtgtctgtt
     1501 tcattgattg gttgcccgtc aaatcggaat ctgatctgcc tcattgacaa tccctgtcgt
     1561 tcacaatagg ctttcattag tttactaagt ggtgtatgcc tcttaatctt aaactgcacc
     1621 acagaaccat cctgccccgc caccttcaaa ttaatatgat cgttgttctc agtcttgact
     1681 ccttccttgg gcttttcgtc ggcgtagtct ggtacgtcgt atgggtaggc atccataagc
     1741 tttctagagt cgacctgcag cccaagcttc gtctaacaaa aaagccaaaa acggccagaa
     1801 tttagcggac aatttactag tctaacactg aaaattacat attgacccaa atgattacat
     1861 ttcaaaaggt gcctaaaaaa cttcacaaaa cacactcgcc aaccccgagc gcatagttca
     1921 aaaccggagc ttcagctact taagaagata ggtacataaa accgaccaaa gaaactgacg
     1981 cctcacttat ccctcccctc accagaggtc cggcgcctgt cgattcagga gagcctaccc
     2041 taggcccgaa ccctgcgtcc tgcgacggag aaaagcctac cgcacaccta ccggcaggtg
     2101 gccccaccct gcattataag ccaacagaac gggtgacgtc acgacacgac gagggcgcgc
     2161 gctcccaaag gtacgggtgc actgcccaac ggcaccgcca taactgccgc ccccgcaaca
     2221 gacgacaaac cgagttctcc agtcagtgac aaacttcacg tcagggtccc cagatggtgc
     2281 cccagcccat ctcacccgaa taagagcttt cccgcattag cgaaggcctc aagaccttgg
     2341 gttcttgccg cccaccatgc cccccacctt gtttcaacga cctcacagcc cgcctcacaa
     2401 gcgtcttcca ttcaagactc gggaacagcc gccattttgc tgcgctcccc ccaaccccca
     2461 gttcagggca accttgctcg cggacccaga ctacagccct tggcggtctc tccacacgct
     2521 tccgtcccac cgagcggccc ggcggccacg aaagccccgg ccagcccagc agcccgctac
     2581 tcaccaagtg acgatcacag cgatccacaa acaagaaccg cgacccaaat cccggctgcg
     2641 acggaactag ctgtgccaca cccggcgcgt ccttatataa tcatcggcgt tcaccgcccc
     2701 acggagatcc ctccgcagaa tcgccgagaa gggactactt ttcctcgcct gttccgctct
     2761 ctggaaagaa aaccagtgcc ctagagtcac ccaagtcccg tcctaaaatg tccttctgct
     2821 gatactgggg ttctaaggcc gagtcttatg agcagcgggc cgctgtcctg agcgtccggg
     2881 cggaaggatc aggacgctcg ctgcgccctt cgtctgacgt ggcagcgctc gccgtgagga
     2941 ggggggcgcc cgcgggaggc gccaaaaccc ggcgcggagg ccagatctgg agccgacacg
     3001 ggttaattaa ccaaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt
     3061 ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc
     3121 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg
     3181 ccatttgtct gcagaattgg cgcacgcagt gccgatccgt tcactaatcg aatggatctg
     3241 tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg
     3301 gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata
     3361 gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat
     3421 tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct
     3481 aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag
     3541 tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc
     3601 caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa
     3661 atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc
     3721 acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc
     3781 tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat
     3841 tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc
     3901 aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt
     3961 ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat
     4021 aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgc
     4081 ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt
     4141 ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa
     4201 tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct
     4261 acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta gctccctgct
     4321 tgcccatact atatgtttta atttatattt tttctttccc cctggcctta accgaatttt
     4381 ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc
     4441 cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct
     4501 tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt
     4561 cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt
     4621 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
     4681 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
     4741 gctcagatct ggtctaacca gagagaccca gtacaggcaa aacgcgctgc ttatatagac
     4801 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
     4861 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
     4921 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
     4981 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
     5041 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     5101 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     5161 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
     5221 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     5281 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
     5341 ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag
     5401 cgcaaaacgc ctaaccctaa gcagattctt catgcaattg tcggtcaagc cttgccttgt
     5461 tgtagcttaa attttgctcg cgcactactc agcgacctcc aacacacaag cagggagcag
     5521 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc ataggggatc
     5581 gggagatctc ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5641 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5701 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5761 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5821 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5881 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5941 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6001 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6061 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6121 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6181 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6241 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6301 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6361 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6421 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6481 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6541 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6601 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6661 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6721 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6781 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6841 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6901 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6961 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7021 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7081 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7141 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7201 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7261 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7321 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7381 cggttcctgg ccttttgttg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7441 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7501 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7561 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7621 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7681 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7741 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga cggtatacag
     7801 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     7861 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     7921 aacaagttgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag
     7981 ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg
     8041 aaatctcgta gcacgtgtca gtcctgctcc tcggccacga agtgcacgca gttgccggcc
     8101 gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt catggccggc
     8161 ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta cagctcgtcc
     8221 aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc ctggaccgcg
     8281 ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc cacgaagtcc
     8341 cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg
     8401 agcaccggaa cggcactggt caacttggcc atggtttagt tcctcacctt gtcgtattat
     8461 actatgccga tatactatgc cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa
     8521 tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     8581 actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa
     8641 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     8701 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     8761 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     8821 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     8881 acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     8941 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     9001 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     9061 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     9121 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     9181 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     9241 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     9301 gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga
     9361 agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt
     9421 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
     9481 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
     9541 cacgggggag gg
//
