LOCUS       sequence-471005-        3839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone with TDP-43-HA insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Suk TR, Part CE, Zhang JL, Nguyen TT, Heer MM, Caballero-Gomez A, 
            Grybas VS, McKeever PM, Nguyen B, Ali T, Callaghan SM, Woulfe JM, 
            Robertson J, Rousseaux MWC
  TITLE     A stress-dependent TDP-43 SUMOylation program preserves neuronal 
            function.
  JOURNAL   Mol Neurodegener. 2025 Mar 28;20(1):38. doi: 
            10.1186/s13024-025-00826-z.
  PUBMED    40149017
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Neurodegener."; date: "2025-03-28"; volume: "20(1):38. doi"; pages: 
            " 10.1186/s13024-025-00826-z"
COMMENT     Sequence Label: pENTR-TDP-43-HA
FEATURES             Location/Qualifiers
     source          1..3839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(115..134)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(116..134)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(139..155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(139..155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(257..276)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             268..1077
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(335..354)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1224..1812
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1713..1732
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1966..1983
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2142..2169)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2261..2347)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2396..2418
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2410..2427
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2411..2427
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2443..2542
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3798..3824
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(join(3838..3839,1..98))
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
       61 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
      121 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
      181 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
      241 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
      301 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
      361 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
      421 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
      481 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
      541 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
      601 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
      661 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
      721 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
      781 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
      841 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
      901 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
      961 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1021 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     1081 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     1141 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     1201 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1261 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1321 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1381 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1441 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1501 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1561 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1621 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1681 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1741 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1801 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1861 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1921 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1981 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2041 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2101 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     2161 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     2221 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     2281 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     2341 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     2401 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt
     2461 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
     2521 actttgtaca aaaaagcagg ctaagcttgc tcgagatgtc tgaatatatt cgggtaaccg
     2581 aagatgagaa cgatgagccc attgaaatac catcggaaga cgatgggacg gtgctgctct
     2641 ccacggttac agcccagttt ccaggggcgt gtgggcttcg ctacaggaat ccagtgtctc
     2701 agtgtatgag aggtgtccgg ctggtagaag gaattctgca tgccccagat gctggctggg
     2761 gaaatctggt gtatgttgtc aactatccaa aagataacaa aagaaaaatg gatgagacag
     2821 atgcttcatc agcagtgaaa gtgaaaagag cagtccagaa aacatccgat ttaatagtgt
     2881 tgggtctccc atggaaaaca accgaacagg acctgaaaga gtattttagt acctttggag
     2941 aagttcttat ggtgcaggtc aagaaagatc ttaagactgg tcattcaaag gggtttggct
     3001 ttgttcgttt tacggaatat gaaacacaag tgaaagtaat gtcacagcga catatgatag
     3061 atggacgatg gtgtgactgc aaacttccta attctaagca aagccaagat gagcctttga
     3121 gaagcagaaa agtgtttgtg gggcgctgta cagaggacat gactgaggat gagctgcggg
     3181 agttcttctc tcagtacggg gatgtgatgg atgtcttcat ccccaagcca ttcagggcct
     3241 ttgcctttgt tacatttgca gatgatcaga ttgcgcagtc tctttgtgga gaggacttga
     3301 tcattaaagg aatcagcgtt catatatcca atgccgaacc taagcacaat agcaatagac
     3361 agttagaaag aagtggaaga tttggtggta atccaggtgg ctttgggaat cagggtggat
     3421 ttggtaatag cagagggggt ggagctggtt tgggaaacaa tcaaggtagt aatatgggtg
     3481 gtgggatgaa ctttggtgcg ttcagcatta atccagccat gatggctgcc gcccaggcag
     3541 cactacagag cagttggggt atgatgggca tgttagccag ccagcagaac cagtcagtcc
     3601 catcgggtaa taaccaaaac caaggcaaca tgcagaggga gccaaaccag gccttcggtt
     3661 ctggaaataa ctcttatagt ggctctaatt ctggtgcagc aattggttgg ggatcagcat
     3721 ccaatgcagg gtcgggcagt ggttttaatg gaggctttgg ctcaagcatg gattctaagt
     3781 cttctggctg gggaatgtac ccatacgacg taccagacta cgcctgaagc ggccgcaac
//
