LOCUS       sequence-471008-        3907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone with TDP-43-SUMO2-HA insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Suk TR, Part CE, Zhang JL, Nguyen TT, Heer MM, Caballero-Gomez A, 
            Grybas VS, McKeever PM, Nguyen B, Ali T, Callaghan SM, Woulfe JM, 
            Robertson J, Rousseaux MWC
  TITLE     A stress-dependent TDP-43 SUMOylation program preserves neuronal 
            function.
  JOURNAL   Mol Neurodegener. 2025 Mar 28;20(1):38. doi: 
            10.1186/s13024-025-00826-z.
  PUBMED    40149017
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Neurodegener."; date: "2025-03-28"; volume: "20(1):38. doi"; pages: 
            " 10.1186/s13024-025-00826-z"
COMMENT     Sequence Label: pENTR-TDP-43-SUMO2-HA
FEATURES             Location/Qualifiers
     source          1..3907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(531..1346)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(531..1346)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1254..1273
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1338..1357
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     1467..1483
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1467..1483
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      1484..1530
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1644..1666
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1658..1675
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1659..1675
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1728..1827
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3340..3366
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(3370..3469)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3526..3542)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3526..3542)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3539..3561)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(join(3714..3907,1..395))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3794..3813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
       61 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      121 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      181 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      241 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      301 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      361 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      421 ggtctgacgc tctattcaac aaagccgccg tcccgtcaag tcagcgtaaa tgggtagggg
      481 gcttcaaatc gtccgctctg ccagtgttac aaccaattaa caaattctga ttagaaaaac
      541 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
      601 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
      661 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
      721 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
      781 gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc
      841 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
      901 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
      961 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     1021 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
     1081 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     1141 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     1201 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     1261 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa
     1321 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     1381 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     1441 gacacaacgt ggctttcccc cgccgctcta gaactagtgg atccaaataa aacgaaaggc
     1501 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcgcattata cgagacgtcc
     1561 aggttgggat acctgaaaca aaacccatcg tacggccaag gaagtctcca ataactgtga
     1621 tccaccacaa gcgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc
     1681 ataatccgca cgcatctgga ataaggaagt gccattccgc ctgacctcaa ataatgattt
     1741 tattttgact gatagtgacc tgttcgttgc aacagattga tgagcaatgc ttttttataa
     1801 tgccaacttt gtacaaaaaa gcaggctatg tctgaatata ttcgggtaac cgaagatgag
     1861 aacgatgagc ccattgaaat accatcggaa gacgatggga cggtgctgct ctccacggtt
     1921 acagcccagt ttccaggggc gtgtgggctt cgctacagga atccagtgtc tcagtgtatg
     1981 agaggtgtcc ggctggtaga aggaattctg catgccccag atgctggctg gggaaatctg
     2041 gtgtatgttg tcaactatcc aaaagataac aaaagaaaaa tggatgagac agatgcttca
     2101 tcagcagtga aagtgaaaag agcagtccag aaaacatccg atttaatagt gttgggtctc
     2161 ccatggaaaa caaccgaaca ggacctgaaa gagtatttta gtacctttgg agaagttctt
     2221 atggtgcagg tcaagaaaga tcttaagact ggtcattcaa aggggtttgg ctttgttcgt
     2281 tttacggaat atgaaacaca agtgaaagta atgtcacagc gacatatgat agatggacga
     2341 tggtgtgact gcaaacttcc taattctaag caaagccaag atgagccttt gagaagcaga
     2401 aaagtgtttg tggggcgctg tacagaggac atgactgagg atgagctgcg ggagttcttc
     2461 tctcagtacg gggatgtgat ggatgtcttc atccccaagc cattcagggc ctttgccttt
     2521 gttacatttg cagatgatca gattgcgcag tctctttgtg gagaggactt gatcattaaa
     2581 ggaatcagcg ttcatatatc caatgccgaa cctaagcaca atagcaatag acagttagaa
     2641 agaagtggaa gatttggtgg taatccaggt ggctttggga atcagggtgg atttggtaat
     2701 agcagagggg gtggagctgg tttgggaaac aatcaaggta gtaatatggg tggtgggatg
     2761 aactttggtg cgttcagcat taatccagcc atgatggctg ccgcccaggc agcactacag
     2821 agcagttggg gtatgatggg catgttagcc agccagcaga accagtcagg cccatcgggt
     2881 aataaccaaa accaaggcaa catgcagagg gagccaaacc aggccttcgg ttctggaaat
     2941 aactcttata gtggctctaa ttctggtgca gcaattggtt ggggatcagc atccaatgca
     3001 gggtcgggca gtggttttaa tggaggcttt ggctcaagca tggattctaa gtcttctggc
     3061 tggggaatga tggacgaaaa gcccaaggaa ggagtcaaga ctgagaacaa cgatcatatt
     3121 aatttgaagg tggcggggca ggatggttct gtggtgcagt ttaagattaa gaggcataca
     3181 ccacttagta aactaatgaa agcctattgt gaacgacagg gattgtcaat gaggcagatc
     3241 agattccgat ttgacgggca accaatcaat gaaacagaca cacctgcaca gttggaaatg
     3301 gaggatgaag atacaattga tgtgttccaa cagcagacgt acccatacga cgtaccagac
     3361 tacgcctgaa cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
     3421 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttga ggctaggtgg
     3481 aggctcagtg atgataagtc tgcgatggtg gatgcatgtg tcatggtcat agctgtttcc
     3541 tgtgtgaaat tgttatccgc tcagagggca caatcctatt ccgcgctatc cgacaatctc
     3601 caagacatta ggtggagttc agttcggcgt atggcatatg tcgctggaaa gaacatgtga
     3661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     3721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3901 ctttctc
//
