LOCUS       sequence-471019-         351 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown expression of Dagla.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Liu Z, Yang N, Dong J, Tian W, Chang L, Ma J, Guo J, Tan J, Dong A, 
            He K, Zhou J, Cinar R, Wu J, Salinas AG, Sun L, Kumar M, Sullivan 
            BT, Oldham BB, Pitz V, Makarious MB, Ding J, Kung J, Xie C, Hawes 
            SL, Wang L, Wang T, Chan P, Zhang Z, Le W, Chen S, Lovinger DM, 
            Blauwendraat C, Singleton AB, Cui G, Li Y, Cai H, Tang B
  TITLE     Deficiency in endocannabinoid synthase DAGLB contributes to early 
            onset Parkinsonism and murine nigral dopaminergic neuron 
            dysfunction.
  JOURNAL   Nat Commun. 2022 Jun 17;13(1):3490. doi: 10.1038/s41467-022-31168-9.
  PUBMED    35715418
REFERENCE   2  (bases 1 to 351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-06-17"; volume: "13(1):3490. doi"; pages: " 
            10.1038/s41467-022-31168-9"
COMMENT     Sequence Label: pAAV-CMV-DIO-SaCas9-U6-sgDagla
FEATURES             Location/Qualifiers
     source          1..351
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     114..133
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        214..289
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
ORIGIN
        1 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acntgacgta
       61 aaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
      121 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      181 gacgaaacac cgatggtcca cagctacgta gaagttttag tactctggaa acagaatcta
      241 ctaaaacaag gcaaaatgcc gtgtttatct cgtcaacttg ttggcgagat ttttcggacc
      301 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg c
//
