LOCUS       sequence-471030-       14511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14511)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 14511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pKEB31
FEATURES             Location/Qualifiers
     source          1..14511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..546
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     447..466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     663..680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1072..1174
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    1487..1508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1523..1528,1529..1546,1547..1553)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1561..1577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1566..1588
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1585..1601
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1585..1601
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1675..1799
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1824..1829,1830..1847,1848..1854)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1908..2567
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2909..3214
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3093..3112
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3255..3379)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     3547..3565
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(3592..3597,3598..3614,3615..3620)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             3668..4858
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     CDS             6912..7541
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7970..9043
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9109..9303
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     13949..13968
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
      601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
      661 gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca
      721 ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc
      781 aacatagtaa gccagtatac actccgctag cgctgatgtc cggcggtgct tttgccgtta
      841 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
      901 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac ccgacgcact
      961 ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt
     1021 ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca
     1081 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
     1141 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1201 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1261 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1321 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1381 tcatcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     1441 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     1501 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     1561 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctag
     1621 aaggagagga ctagatggat ggatggatca tcaagcttat cgataccgtc atcaacaagt
     1681 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
     1741 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
     1801 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
     1861 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     1921 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     1981 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     2041 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     2101 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
     2161 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     2221 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     2281 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     2341 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     2401 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     2461 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     2521 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg
     2581 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
     2641 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
     2701 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
     2761 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
     2821 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
     2881 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
     2941 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
     3001 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
     3061 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
     3121 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
     3181 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
     3241 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
     3301 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
     3361 ctttcttgta caaagtggtt gatgacctcg aattattgaa gcatttatca gggttattgt
     3421 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3481 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     3541 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattctcatg tttgacagct
     3601 tatcatcgat aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc
     3661 accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc
     3721 tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc
     3781 cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct
     3841 atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc
     3901 ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat
     3961 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     4021 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     4081 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     4141 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     4201 cttcctaatg caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa
     4261 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     4321 cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga
     4381 ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt
     4441 gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca
     4501 ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac
     4561 gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc
     4621 cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg
     4681 atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat
     4741 ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata
     4801 ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat
     4861 ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc
     4921 ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc
     4981 atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc
     5041 atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag
     5101 cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg
     5161 acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg
     5221 aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc
     5281 tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc
     5341 tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca
     5401 tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc
     5461 cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc
     5521 cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag
     5581 ctggacgcgg atgaacaggc agacatctgt gaatccatga gcccgaagtg gcgagcccga
     5641 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     5701 atgccggcca cgatgcgtcc ggcgtagagg atcgatccaa cccctccgct gctatagtgc
     5761 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta
     5821 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
     5881 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
     5941 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
     6001 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
     6061 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
     6121 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
     6181 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
     6241 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
     6301 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt
     6361 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
     6421 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
     6481 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
     6541 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
     6601 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
     6661 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc
     6721 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
     6781 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     6841 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     6901 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     6961 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     7021 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     7081 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     7141 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     7201 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     7261 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     7321 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     7381 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     7441 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     7501 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     7561 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     7621 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     7681 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     7741 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     7801 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     7861 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     7921 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
     7981 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     8041 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     8101 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     8161 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     8221 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     8281 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     8341 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
     8401 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     8461 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     8521 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     8581 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     8641 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     8701 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     8761 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     8821 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     8881 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     8941 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     9001 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     9061 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac
     9121 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     9181 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     9241 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     9301 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     9361 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     9421 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     9481 ccgccggcgc ccacatcaag gcaccgcggc tgctgaaaga ccgtcaaaag cggtttagca
     9541 gtgaaaagca gggctgccca atcacctgcg ctcgactgga acgccggtgg tggctgaaag
     9601 gccgcaaaag ttgaacgcca aggaaaaggg cagggcgaca gcccagggca tcacctacgg
     9661 ccaccaggcg tgacaggagc gccgaacacg cccaaagccg ggggattgcc gcaccgaccc
     9721 ctgaaaccgc cgctacgggc ttcttacgcg gttttagccg tggctgattt cctgaaccgt
     9781 cgccagcacg ccggcggccg ggcaaaataa taacttgcat aataagaata agaagattat
     9841 tattattctt atcttattta tccagccggg aggcaccgcc atgacgcaaa ccaatatccc
     9901 cgccgacgtg cgcgaacgca tcattgccgc tgccgccgag ctgtacgaac aggccggccg
     9961 cgagaacttg ccgaccgttg acgccgtgcg ccgcgcagcg cgggctgaca tgaacacaac
    10021 cagcgccgtg atgcgggaat ggcggcgagc gcagaccgcc caggcggccc cggtggccgt
    10081 caccgtgccg gaggtcgtca cccaagcgaa caccgccgcc ctggccgcct tatggcagca
    10141 ggcccaggaa ctggcgaacg agtcattgcg ggccgcgcaa gcggcatggg aaaccgagcg
    10201 tgtcgagctg gacaccatgc gccaagagct ggccgacgcc tacgaaaccc aggcaaccga
    10261 gcttgaccag gtgcgggccg ccgccgaagc ggccgaccag gcgcaccagg tacaaagtca
    10321 gcaggtcgcc ggcgagctgg ccagcgtgcg gacagaattg gcccaggctg tcaccagggc
    10381 cgaacgtgcg gaagcgaagg ccgacgagat cgagcaccgt gcccgcgagc tgcgggccga
    10441 gctggacagg gcacaccagg acgccgacca ggcgcgggct gccgtgaccg atgcgcagca
    10501 ggctacccag gcagtgaccg cgcaactcga ccaggtacgc gccgaactgg cgacggtcaa
    10561 agccaaggca gaggccgccg acgagaagca ccaggagcag cgaaaggcgg tcgcggtcga
    10621 ggcgcacaag atggccgaac ggctgaccgc gacccaggcc gaacgcgacc aggccagaga
    10681 ggaagccggc agcgcccgcg agcgagccgc cgagctggcc ggccagctca ccgcacacca
    10741 ggaacaggcg gccgcgttgc tggcacgcct ggcaccggcc gaaaccaaaa cacccagggc
    10801 gaagaagtag ggcggtggat tggtctggcg gtagtgattg aagtgtcaac aaagagccgc
    10861 tccgcccctc aagtgtcagt aggccgccgc tatggcgagg ggctgatagc gaccggcgtt
    10921 tttaggtgtt cattgacact tgaggggcgg ggtgtaccgc ctctttcctt ggcgtccaac
    10981 cttgtttttt tgggcggtca tgtggccgtg gaaagcatgg ggaaagtggt gtttcctcgt
    11041 tgtggaaaca gcaaggaaat cgttgctttc ttgtgcaaag cagagttttg gaaaattcga
    11101 ttaaaccgcg attttggcac ggtttaatgc cgatttaagc ccgtttttcc cgctgtagag
    11161 cccctattag gccgattaca ggccgcttta acccgattgc atggcaatcg gtatgcgata
    11221 gcgcatgaat tgcacaagaa tccaactgct gtactatgag tattcgaggc tgaatagcgg
    11281 gcaggatgtg tgaagtaggc ccacccgtaa acgggttgtc cttcttcact tccccttatt
    11341 cgcacctgcg gtgctcaacg ggaatcctgc cctgcggggc cagccggcag cgccggccaa
    11401 agagacgata ggagggcttg ccgatggcgg cagagaacga caaaccaacg cgcaagggca
    11461 gcccgccgat caaggtgtat tgcctgcccg aagagcgggc cgagatcgag gcgctggcga
    11521 aggccagcgg ccacagcgca tcgacttatt tgcgcttggt tgggcagggc taccaggtca
    11581 ccggcgtgac cgattacgag aacgtgcgcg agctggtgcg cgtgaacggc gaccttggcc
    11641 ggctgggtgg tctgctgaaa ctttggctga ccgatgacgc caaaactgcc gaaataggcg
    11701 cggccaccat ccgggcgctg ttgggccgca tcgaggccaa ccaggacgaa atgagccgcc
    11761 ttatggaagc tgtggtgcgg ccgagggccg cgccatgatt gccaagcacg tcgccatgcg
    11821 gtcgcttggc aagagcgatt ttgccgacct ggcgaagtac atcaccgatg cgcagagcaa
    11881 agaccatcgg ctgggcttgg tggccgtgac caactgcgag gcgttttcag tgcgggacgc
    11941 catcaccgaa gttctggcca cccagcacac caacacccgc gcccagggtg ataagaccta
    12001 tcacctgatc gtgagctttc gcgccggcga aaaccccgac gccgacacgc tcaaggcaat
    12061 agaggcgcgt atctgcgagg ggctgggctt cggcgaacac cagcgcatca gcgccgtgca
    12121 tcacgacacc gacaacctgc atatccatat cgccatcaac aagattcacc cgacccggca
    12181 cacgatgcac gagccgtatt acccgcacct ggcacttggc gagctatgca cgaagctgga
    12241 acaggaatac ggcctggaac gcgacaacca cgaaccgcgc caacggggtg cggcgtccag
    12301 ggcggcggac atggagcgtc atgccggcgt tgaaagcctg gtgggctgga tcaagcgcga
    12361 gtgcttggcc gagatcaagg gcgcgcagac ctggcaggag ctgcaccagg tcatgcgtga
    12421 taacgggctg gaaatcacgc ctcgcggtaa cggcctggtg atccgggccg aggacggtac
    12481 gaccgtcaag ccaagcacgc ttgggcggga tctatccaag ccagctttgg aaaaacggct
    12541 gggcactttt gagccgtcgc ccgagcggca ggcagagccg aaacggcgat acaagaaaga
    12601 cccgatccgc ttgcgcgtga acacggtcga gttgtacgcc aggtataagg ctgaccagca
    12661 gaacctgacg gccagccggg cgaaggccct ggacgtggcc aggcagcgca aggacaggaa
    12721 agttgcagcg gccaaggcat cggggagcat gcgccgcgct gcgatcaaac atatgacgca
    12781 aggccggctg accaagaagc tgttgtattc gcaggccagc aaggcgctgc gcgatgaaat
    12841 tcagacgatc aacaagcagt acctggcaga gcgtcagggc tgctatgagc agcacagccg
    12901 gcgcacatgg gccgactggc tcaagcagga agccacccag ggaagcggcg aggcgctggc
    12961 cgcgttgcga gccagagagg ccgcccaggg cctcaagggc aacaccgtcc agggcgatgg
    13021 ccaggccaag cccggacatg cgcccgtggt ggacaacatc acgaagaagg gcacgatcat
    13081 tttccgggcc ggcaagactg ccgtacgtga cgatggcgag cgcctgcaag tgtcccgaga
    13141 agccagccgc gagggcctgc aagaagcctt gcgccttgcg atggagcgat atggcaaccg
    13201 catcacggtc aacggcacga ccgagttcaa ggcgcagatc atccgcgccg ccgtcgattc
    13261 acaactaccc atcacgtttt ccgacccggt cctggaagtc cgcaggcagg cgctgatagg
    13321 cggtagtgat acgcctgcca agccgacgat caagaagcca ggcgttggcc cggtagggca
    13381 cgttccgccg gcagcgcgtc agggccgatt gcgcacgttg tcgcagatcg aggcgctgca
    13441 catcgagggc gcgagcatcc caacgccgaa gcctgcaccg attccggcaa tcaatgacca
    13501 ggaacggcga aaagcgaagc tgcaagccga gttgaacgcc aagaaagaga aggcgaaaca
    13561 gcggaaagga cgggggcggt agttttaacc tttttgagta aaaacagctt gcatcatttg
    13621 agtttggtag atatgatgca atgagtatgc aatcatttat gtaaagcagt aatcgtgcag
    13681 ggttgcacga cacacaccga tctcaacaaa aaattgcaag ggcgcgacgg tgtcgatcga
    13741 ccaaagcggc catcgtgcct ccccactcct gcagttcggg ggcatggatg cgcggatagc
    13801 cgctgctggt ttcctggatg ccgacggatt tgcactgccg gtagaactcc gcgaggtcgt
    13861 ccagcctccg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
    13921 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
    13981 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
    14041 tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat
    14101 ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg
    14161 ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc
    14221 tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt
    14281 catccggggt cagcaccgtt tctgcggact ggctttctac gtgttccgct tcctttagca
    14341 gcccttgcgc cctgagtgct tgcggcagcg tgaagctgct tatcgatgat aagctgtcaa
    14401 acatgagaat tacaacttat atcgtatggg gctgacttca ggtgctacat ttgaagagat
    14461 aaattgcact gaaatctaga aatattttat ctgattaata agatgatctt c
//
