LOCUS       sequence-471034-        5129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: IL10RB_pCSdest
FEATURES             Location/Qualifiers
     source          1..5129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    107..131
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1113..1137)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    1181..1315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1205..1224)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1259..1278
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1428..1448)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1428..1446)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1467..1483)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1467..1483)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1480..1502)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1491..1507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1515..1521,1522..1539,1540..1545))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1560..1581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1698..1715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1869..2457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1949..1968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2628..3419,3420..3488))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3251..3270
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3489..3593)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3619..4074)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3756..3777)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3968..3987
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4145..5129
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggatcacaa gtttgtacaa
      121 aaaagcaggc accatggcgt ggagccttgg gagctggctg ggtggctgcc tgctggtgtc
      181 agcattggga atggtaccac ctcccgaaaa tgtcagaatg aattctgtta atttcaagaa
      241 cattctacag tgggagtcac ctgcttttgc caaagggaac ctgactttca cagctcagta
      301 cctaagttat aggatattcc aagataaatg catgaatact accttgacgg aatgtgattt
      361 ctcaagtctt tccaagtatg gtgaccacac cttgagagtc agggctgaat ttgcagatga
      421 gcattcagac tgggtaaaca tcaccttctg tcctgtggat gacaccatta ttggaccccc
      481 tggaatgcaa gtagaagtac ttgctgattc tttacatatg cgtttcttag cccctaaaat
      541 tgagaatgaa tacgaaactt ggactatgaa gaatgtgtat aactcatgga cttataatgt
      601 gcaatactgg aaaaacggta ctgatgaaaa gtttcaaatt actccccagt atgactttga
      661 ggtcctcaga aacctggagc catggacaac ttattgtgtt caagttcgag ggtttcttcc
      721 tgatcggaac aaagctgggg aatggagtga gcctgtctgt gagcaaacaa cccatgacga
      781 aacggtcccc tcctggatgg tggccgtcat cctcatggcc tcggtcttca tggtctgcct
      841 ggcactcctc ggctgcttcg ccttgttgtg gtgcgtttac aagaagacaa agtacgcctt
      901 ctcccctagg aattctcttc cacagcacct gaaagagttt ttgggccatc ctcatcataa
      961 cacacttctg tttttctcct ttccattgtc ggatgagaat gatgtttttg acaagctaag
     1021 tgtcattgca gaagactctg agagcggcaa gcagaatcct ggtgacagct gcagcctcgg
     1081 gaccccgcct gggcaggggc cccaaagcta gaacccagct ttcttgtaca aagtggtgat
     1141 cctctcgagc ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata
     1201 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     1261 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     1321 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt
     1381 cgcggccgcg gcgccaatgc attgggcccg gtacccagct tttgttccct ttagtgaggg
     1441 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     1501 ctcacaattc cacacaacat acgagccggg agcataaagt gtaaagcctg gggtgcctaa
     1561 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1621 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1681 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1741 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1801 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1861 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1921 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1981 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2041 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2101 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2161 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2221 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2281 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2341 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2401 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2461 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2521 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2581 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2641 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2701 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2761 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2821 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2881 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2941 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3001 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3061 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3121 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     3181 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3241 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3301 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3361 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3421 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3481 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3541 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3601 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     3661 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     3721 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     3781 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     3841 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     3901 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     3961 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     4021 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
     4081 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     4141 gccagtcgac cgccaattca atatggcgta tatggactca tgccaattca atatggtgga
     4201 tctggacctg tgccaattca atatggcgta tatggactcg tgccaattca atatggtgga
     4261 tctggacccc agccaattca atatggcgga cttggcacca tgccaattca atatggcgga
     4321 cctggcactg tgccaactgg ggaggggtct acttggcacg gtgccaagtt tgaggagggg
     4381 tcttggccct gtgccaagtc cgccatattg aattggcatg gtgccaataa tggcggccat
     4441 attggctata tgccaggatc aatatatagg caatatccaa tatggcccta tgccaatatg
     4501 gctattggcc aggttcaata ctatgtattg gccctatgcc atatagtatt ccatatatgg
     4561 gttttcctat tgacgtagat agcccctccc aatgggcggt cccatatacc atatatgggg
     4621 cttcctaata ccgcccatag ccactccccc attgacgtca atggtctcta tatatggtct
     4681 ttcctattga cgtcatatgg gcggtcctat tgacgtatat ggcgcctccc ccattgacgt
     4741 caattacggt aaatggcccg cctggctcaa tgcccattga cgtcaatagg accacccacc
     4801 attgacgtca atgggatggc tcattgccca ttcatatccg ttctcacgcc ccctattgac
     4861 gtcaatgacg gtaaatggcc cacttggcag tacatcaata tctattaata gtaacttggc
     4921 aagtacatta ctattggaag tacgccaggg tacattggca gtactcccat tgacgtcaat
     4981 ggcggtaaat ggcccgcgat ggctgccaag tacatcccca ttgacgtcaa tggggagggg
     5041 caatgacgca aatgggcgtt ccattgacgt aaatgggcgg taggcgtgcc taatgggagg
     5101 tctatataag caatgctcgt ttagggaac
//
