LOCUS       sequence-471036-        9408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Quayle SN, Chheda MG, Shukla SA, Wiedemeyer R, Tamayo P, Dewan RW, 
            Zhuang L, Huang-Hobbs E, Haidar S, Xiao Y, Ligon KL, Hahn WC, Chin L
  TITLE     Integrative functional genomics identifies RINT1 as a novel GBM 
            oncogene.
  JOURNAL   Neuro Oncol. 2012 Nov;14(11):1325-31. doi: 10.1093/neuonc/nos246. 
            Epub 2012 Oct 16.
  PUBMED    23074196
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/neuonc/nos246"; journalName: "Neuro Oncol"; date: 
            "2012-11"; volume: "14"; issue: "11"; pages: "1325-31"
COMMENT     Sequence Label: pLenti6/V5-DEST-RINT1
FEATURES             Location/Qualifiers
     source          1..9408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    625..649
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3026..3050)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3103..3144
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(3181..3201)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3186..3515
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3366..3501
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3563..3610
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3629..4027
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4114..4347
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4419..4540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4456..4475)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4510..4529
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4580..4715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4735..4754)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4736..4754)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4764..4781)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4764..4780)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4773..4795)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4922..5377
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5009..5028)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5219..5240
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5403..5507
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5508..5576,5577..6368)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5726..5745)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6539..7127
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7028..7047
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7281..7298
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7415..7436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7451..7456,7457..7474,7475..7481)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7489..7505
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7494..7516
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7513..7529
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7513..7529
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7548..7568
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7550..7568
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7596..7822
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7823..8003
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8050..8175
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8668..8901
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9086..9130
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagaggatcc actagtccag
      601 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagttggcat gctaccagcc
      661 ggcgagatcg gcgcctctcc tgcagccccg tgctgctctg aaagtggtga cgaaaggaag
      721 aacctcgagg agaaaagtga cataaatgtt acagttctta ttggaagtaa acaagtcagt
      781 gaaggtacag ataatggtga tctcccttct tatgtgtctg cattcataga aaaggaagtt
      841 ggaaatgacc ttaaatcttt aaagaaactt gataaactca tagaacagag gacagtaagt
      901 aaaatgcagt tagaagaaca ggtacttaca atttcatcag aaattcctaa aagaattcga
      961 agtgccttaa aaaatgcaga agaatcaaag caatttctta atcagtttct ggagcaggaa
     1021 actcatctct tcagcgccat taacagccat ttgctgactg cgcaaccttg gatggacgat
     1081 cttggaacca tgattagcca gattgaagag atcgaacgtc atcttgctta ccttaaatgg
     1141 atttcacaaa ttgaagaact aagtgataac attcagcaat atctgatgac caataatgta
     1201 ccggaggcag cctccactct agtgtctatg gcagaacttg acattaaact tcaggaatca
     1261 tcttgtactc atcttcttgg tttcatgaga gccacagtta aattctggca taaaattctc
     1321 aaggacaagc ttacaagtga ttttgaggaa attttagcac agcttcattg gccattcatc
     1381 gcaccccctc aatcacaaac tgttggctta agtcgacctg ccagtgcccc ggagatatac
     1441 agttacctgg agacaatgtt ttgtcagctt ttgaaactac aaacctcaga tgaattactt
     1501 actgagccaa agcaactccc agaaaaatac tctcttcctg cctccccttc tgtcatcctg
     1561 cccatccagg ttatgctgac tcctcttcag aagaggttca ggtatcactt cagagggaac
     1621 cggcagacta atgtgttaag caagccagaa tggtacttgg ctcaagtact tatgtggatt
     1681 ggaaaccata ctgaatttct ggatgagaag attcagccaa tattagacaa agtaggctct
     1741 ttggtaaacg caaggcttga attttctcgg ggccttatga tgctggttct tgagaagtta
     1801 gccactgata ttccttgtct gctatatgat gacaatctct tctgtcattt ggtggatgaa
     1861 gtactcttgt ttgaaaggga gctacacagt gttcatggct atcctggcac ttttgctagt
     1921 tgtatgcata ttctatcaga ggaaacctgt tttcagagat ggttgacggt ggagagaaaa
     1981 tttgctcttc aaaaaatgga ctcaatgctt tcctcagaag ctgcctgggt atcgcaatat
     2041 aaggatatca ctgacgtgga tgaaatgaaa gttccagatt gtgcagaaac ttttatgact
     2101 ctactcttgg ttataactga caggtataaa aatcttccca cagcttcccg aaagcttcag
     2161 ttcctggagt tacagaagga cttagtagat gattttagga tacgattaac acaagtgatg
     2221 aaagaagaga ctagagcttc ccttggcttt cgatactgtg caattcttaa tgctgtgaac
     2281 tacatctcaa cagtactagc agattgggct gacaatgttt tctttctaca acttcaacag
     2341 gctgcactgg aggtgtttgc agagaataat actctgagta aattgcagct aggacagcta
     2401 gcctctatgg agagctctgt ctttgatgac atgattaacc tcttagaacg tttaaagcat
     2461 gatatgttga cccgtcaagt agaccacgtt tttagagaag ttaaagatgc tgcaaaattg
     2521 tataaaaaag aaagatggtt gtccttgcca tctcagtcag agcaggcagt gatgtccctg
     2581 tccagttcgg cttgcccgtt gctgctgacg ttacgagacc atttacttca gttggagcag
     2641 cagctttgtt tctccttatt taaaattttc tggcaaatgc ttgtagagaa gctggatgta
     2701 tacatctacc aagaaataat tcttgctaat cacttcaatg aaggaggagc agcccagctg
     2761 cagtttgata tgactcggaa tcttttccct ttgttttctc actattgcaa gagaccagaa
     2821 aattatttta aacatataaa agaagcctgt attgttttga atttgaacgt cggttctgca
     2881 ctactgctga aagatgtact gcagtcagct tcagggcagc ttcctgccac agcagcatta
     2941 aatgaagttg gaatttacaa actggctcaa caagatgttg agattctact taatttgagg
     3001 acaaattggc ctaatactgg aaaataccca actttcttgt acaaagtggt tgatatccag
     3061 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
     3121 cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagtttggaa ttaattctgt
     3181 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3241 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     3301 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     3361 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     3421 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     3481 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     3541 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     3601 ataatacgac aaggtgagga actaaaccat ggccaagcct ttgtctcaag aagaatccac
     3661 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     3721 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     3781 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     3841 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     3901 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     3961 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     4021 gggctaagca caattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     4081 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     4141 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4201 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4261 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4321 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     4381 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     4441 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4501 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     4561 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4621 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4681 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     4741 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4801 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4861 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4921 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4981 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5041 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5101 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5161 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5221 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5281 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5341 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     5401 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5461 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5521 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5581 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5641 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5701 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5761 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5821 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5881 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5941 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6001 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6061 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6121 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6181 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6241 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6301 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6361 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6421 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6481 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6541 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6601 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6661 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6721 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6781 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6841 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6901 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6961 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7021 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7081 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7141 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7201 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7261 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7321 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7381 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7441 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7501 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     7561 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     7621 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     7681 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     7741 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     7801 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     7861 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7921 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7981 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     8041 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     8101 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     8161 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     8221 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     8281 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     8341 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     8401 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     8461 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     8521 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     8581 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     8641 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     8701 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8761 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8821 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8881 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8941 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     9001 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     9061 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     9121 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     9181 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     9241 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     9301 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     9361 tcgattagtg aacggatctc gacggtatcg ataagcttgg gagttccg
//
