LOCUS       sequence-471043-        3658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector to store Level 0 resistance cassette parts and E. coli 
            shuttle part (containing no E. coli ori).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Stukenberg D, Hensel T, Hoff J, Daniel B, Inckemann R, Tedeschi JN, 
            Nousch F, Fritz G
  TITLE     The Marburg Collection: A Golden Gate DNA Assembly Framework for 
            Synthetic Biology Applications in Vibrio natriegens.
  JOURNAL   ACS Synth Biol. 2021 Aug 20;10(8):1904-1919. doi: 
            10.1021/acssynbio.1c00126. Epub 2021 Jul 13.
  PUBMED    34255476
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00126"; journalName: "ACS Synth Biol"; date: 
            "2021-08-20- 20"; volume: "10"; issue: "8"; pages: "1904-1919"
COMMENT     Sequence Label: pLP2
FEATURES             Location/Qualifiers
     source          1..3658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(96..684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(176..195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(881..975)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(996..1655)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1570..1589
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1817..1835
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     mobile_element  2016..2783
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     RBS             2787..2798
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3512..3583
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3599..3626
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
       61 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      121 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      181 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      241 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      301 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      361 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      421 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      481 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      541 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      601 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      661 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      721 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      781 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      841 acttggtctg acagctcgag gcttggggtc tcgtgagacg attctcacca ataaaaaacg
      901 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
      961 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcgtcg
     1021 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     1081 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     1141 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     1201 ctcacccagg gattggctga tacgaaaaac atattctcaa taaacccttt agggaaatag
     1261 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     1321 tcgtcgtggt attcactcca gagtgatgaa aacgtttcag tttgctcatg gaaaacggtg
     1381 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     1441 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     1501 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     1561 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     1621 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     1681 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     1741 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat
     1801 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagcgtct catgagaccc
     1861 ggaaagtgaa acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt atttaataat
     1921 gtgtgcggca attcacattt aatttatgaa tgttttctta acatcgcggc aactcaagaa
     1981 acggcaggtt cggatcttag ctactagaga aagagggtga tgctgccaac ttactgattt
     2041 agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag ctgtccctcc
     2101 tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctat
     2161 ctctgctctc actgccgtaa aacatggcaa ctgcagttca cttacaccgc ttctcaaccc
     2221 ggtacgcacc agaaaatcat tgatatggcc atgaatggcg ttggatgccg ggcaacagcc
     2281 cgcattatgg gcgttggcct caacacgatt ttacgtcact taaaaaactc aggccgcagt
     2341 cggtaacctc gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa atggacgaac
     2401 agtggggcta tgtcggggct aaatcgcgcc agcgctggct gttttacgcg tatgacagtc
     2461 tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc
     2521 ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc tggccgctgt
     2581 atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg cagcgaattg
     2641 agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag tcgctgtcgt
     2701 tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac ataaaacact
     2761 atcaataagt tggagtcatt accgagaaag aggagaaata ctagatggtt tctaaaggtg
     2821 aagcagtgat caaagaattt atgcgcttca aagttcacat ggaaggttct atgaacggcc
     2881 acgaatttga aattgaaggt gaaggcgaag gtcgtccata cgaaggtact caaaccgcaa
     2941 aactgaaagt taccaaaggt ggtccactgc cattttcttg ggatattctg tctccacaat
     3001 ttatgtacgg ttctcgtgca tttatcaaac acccagcaga tattccagac tactacaaac
     3061 aatcttttcc ggaaggtttc aaatgggaac gtgttatgaa ttttgaagat ggtggtgcag
     3121 ttacggttac ccaagatacc tctctggaag atggtactct gatctacaaa gttaaactgc
     3181 gtggtactaa ctttccacca gatggtccag ttatgcagaa aaaaaccatg ggttgggaag
     3241 catctaccga acgtctgtac ccagaagatg gcgttctgaa aggtgatatc aaaatggcac
     3301 tgcgtctgaa agatggcggt cgttacctgg cagatttcaa aaccacctac aaagcgaaaa
     3361 aaccagttca aatgccaggt gcatacaacg ttgatcgtaa actggatatt accagccaca
     3421 acgaagatta caccgttgtt gaacaatacg aacgttctga aggccgtcac tctaccggtg
     3481 gtatggatga actgtacaaa taaccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     3541 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     3601 ctcaccttcg ggtgggcctt tctgcgttta tacagctcac tcaaaggcgg taatacgg
//
