LOCUS       sequence-471048-        4933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2114m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP1634 - pAAV-AiE2114m-minBG-iCre-HGHpA
FEATURES             Location/Qualifiers
     source          1..4933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      599..608
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             611..631
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1680..2156
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1879..1898)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2196..2336
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2196..2325
                     /label=AAV2 ITR
     rep_origin      2411..2866
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2498..2517)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2708..2729
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2883..2902)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3002..3024
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3062..3080)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3148..3252
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3253..3321,3322..4113)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3471..3490)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4284..4872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4773..4792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttggca tttgacattt acctgaagtc tgtcttgtgc
      181 cgggtcctgt ccccaggttg ggtctaagtc tcacgctgga tctcagtgct cacagcttcc
      241 tccagaacat acctgctctg gcattatctt tcctgagcag atgctagaac ttggctcaga
      301 gtgatgaaga ggaagtgtgt cctctggcta aagtccaggg gttgcagagg ggagtgtgtg
      361 agatatttta atacatacct atcttgagaa ggtagcaggg gtgttaaagc atatgtaatc
      421 atccaggctt cttcatatct caagaaatct agcatagcac aaagatgggc tagatgtacc
      481 caaaggtgca tgcaaaacgt aagcagcttc ttgtttccta tttcactgag tttgagctcg
      541 ggctgggcat aaaagtcagg gcagagccat ctattgctta catttgcttc tgggatccgc
      601 caccatggtg cccaagaaga agaggaaagt ctccaacctg ctgactgtgc accaaaacct
      661 gcctgccctc cctgtggatg ccacctctga tgaagtcagg aagaacctga tggacatgtt
      721 cagggacagg caggccttct ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc
      781 ctgggctgcc tggtgcaagc tgaacaacag gaaatggttc cctgctgaac ctgaggatgt
      841 gagggactac ctcctgtacc tgcaagccag aggcctggct gtgaagacca tccaacagca
      901 cctgggccag ctcaacatgc tgcacaggag atctggcctg cctcgccctt ctgactccaa
      961 tgctgtgtcc ctggtgatga ggagaatcag aaaggagaat gtggatgctg gggagagagc
     1021 caagcaggcc ctggcctttg aacgcactga ctttgaccaa gtcagatccc tgatggagaa
     1081 ctctgacaga tgccaggaca tcaggaacct ggccttcctg ggcattgcct acaacaccct
     1141 gctgcgcatt gccgaaattg ccagaatcag agtgaaggac atctcccgca ccgatggtgg
     1201 gagaatgctg atccacattg gcaggaccaa gaccctggtg tccacagctg gtgtggagaa
     1261 ggccctgtcc ctgggggtta ccaagctggt ggagagatgg atctctgtgt ctggtgtggc
     1321 tgatgacccc aacaactacc tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc
     1381 tgccacctcc caactgtcca cccgggccct ggaagggatc tttgaggcca cccaccgcct
     1441 gatctatggt gccaaggatg actctgggca gagatacctg gcctggtctg gccactctgc
     1501 cagagtgggt gctgccaggg acatggccag ggctggtgtg tccatccctg aaatcatgca
     1561 ggctggtggc tggaccaatg tgaacattgt gatgaactac atcagaaacc tggactctga
     1621 gactggggcc atggtgaggc tgctcgagga tggggactaa ggctgctcga gagatctacg
     1681 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     1741 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     1801 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
     1861 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     1921 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     1981 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     2041 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     2101 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
     2161 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     2221 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     2281 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     2341 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     2401 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2461 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
     2521 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2581 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     2641 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     2701 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
     2761 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
     2821 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     2881 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     2941 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3001 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     3061 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3121 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3181 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3241 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3301 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3361 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3421 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3481 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3541 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3601 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3661 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3721 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3781 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3841 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3901 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3961 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4021 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4081 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4141 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4201 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4261 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4321 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4381 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4441 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4501 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4561 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4621 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4681 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4741 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4801 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4861 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4921 tttgctcaca tgt
//
