LOCUS       sequence-471049-        5158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2116m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP1636 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA
FEATURES             Location/Qualifiers
     source          1..5158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      824..833
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             836..856
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1905..2381
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2104..2123)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2421..2561
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2421..2550
                     /label=AAV2 ITR
     rep_origin      2636..3091
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2723..2742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2933..2954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3108..3127)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3227..3249
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3287..3305)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3373..3477
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3478..3546,3547..4338)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3696..3715)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4509..5097
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4998..5017
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttcacac ctggacgaca cctgtgaagg tcactgtcct
      181 tcactcacac ctggacacct gtgaaggtta ctgtcctcca ctcacacctg aatgacacct
      241 gtgaaagtca aggtcacaca aaaaggcact tttgtgctta tgcttctgtt ttaatctggg
      301 aacccagatg ttctatcagc atgctttatt ctgaggcccc cgcacatcct tcatgtatgc
      361 aagccttctc ttatgtaacc ttttggcttt ctgtggccag atggcttcct ctttcattca
      421 tccctatgtg aaccaactat gactccaaaa tatctggcct ttgtgtatca catatactcc
      481 agctttctgg tcttttttag gttccttgaa atatgccagg ctgtttcctc ttcctggggc
      541 ttcacagtag cagtctggta tacctgggat attttttctc ttatcctcat tatatgtgtt
      601 cctttctaaa tgtcacttct catggaatcc actttaccta atttgtgtag attttcagaa
      661 tttatgccca tgccaaagat ctttaccata actggatcta atcaccttta tgaaggtaag
      721 aaaagatccc aaatttattt agctatttca ctgagtttga gctcgggctg ggcataaaag
      781 tcagggcaga gccatctatt gcttacattt gcttctggga tccgccacca tggtgcccaa
      841 gaagaagagg aaagtctcca acctgctgac tgtgcaccaa aacctgcctg ccctccctgt
      901 ggatgccacc tctgatgaag tcaggaagaa cctgatggac atgttcaggg acaggcaggc
      961 cttctctgaa cacacctgga agatgctcct gtctgtgtgc agatcctggg ctgcctggtg
     1021 caagctgaac aacaggaaat ggttccctgc tgaacctgag gatgtgaggg actacctcct
     1081 gtacctgcaa gccagaggcc tggctgtgaa gaccatccaa cagcacctgg gccagctcaa
     1141 catgctgcac aggagatctg gcctgcctcg cccttctgac tccaatgctg tgtccctggt
     1201 gatgaggaga atcagaaagg agaatgtgga tgctggggag agagccaagc aggccctggc
     1261 ctttgaacgc actgactttg accaagtcag atccctgatg gagaactctg acagatgcca
     1321 ggacatcagg aacctggcct tcctgggcat tgcctacaac accctgctgc gcattgccga
     1381 aattgccaga atcagagtga aggacatctc ccgcaccgat ggtgggagaa tgctgatcca
     1441 cattggcagg accaagaccc tggtgtccac agctggtgtg gagaaggccc tgtccctggg
     1501 ggttaccaag ctggtggaga gatggatctc tgtgtctggt gtggctgatg accccaacaa
     1561 ctacctgttc tgccgggtca gaaagaatgg tgtggctgcc ccttctgcca cctcccaact
     1621 gtccacccgg gccctggaag ggatctttga ggccacccac cgcctgatct atggtgccaa
     1681 ggatgactct gggcagagat acctggcctg gtctggccac tctgccagag tgggtgctgc
     1741 cagggacatg gccagggctg gtgtgtccat ccctgaaatc atgcaggctg gtggctggac
     1801 caatgtgaac attgtgatga actacatcag aaacctggac tctgagactg gggccatggt
     1861 gaggctgctc gaggatgggg actaaggctg ctcgagagat ctacgggtgg catccctgtg
     1921 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
     1981 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
     2041 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg
     2101 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
     2161 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
     2221 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
     2281 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
     2341 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca
     2401 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     2461 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     2521 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     2581 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     2641 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2701 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2761 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2821 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     2881 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2941 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
     3001 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3061 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     3121 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3181 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3241 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     3301 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     3361 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3421 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3481 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3541 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3601 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3661 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3721 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3781 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3841 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3901 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3961 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4021 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4081 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4141 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4201 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4261 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4321 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4381 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4441 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4501 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4561 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4621 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     4681 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4741 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4801 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4861 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4921 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4981 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5041 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5101 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
