LOCUS       sequence-471055-        9785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid pQdCas9.luxR(mut)-sggfp expresses the dCas9 endonuclease
            and the sgRNA targeting the GFP gene. Additionally, this plasmid 
            contains a mutation in the luxR gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Golla SA, Abo-Hashesh M, Gupta D, Liu Y, Mahadevan R
  TITLE     Model-Based Optimization of a qCRISPRi Circuit for Dynamic Control 
            of Metabolic Pathways.
  JOURNAL   ACS Synth Biol. 2025 Oct 17;14(10):3890-3898. doi: 
            10.1021/acssynbio.5c00095. Epub 2025 Sep 29.
  PUBMED    41021780
REFERENCE   2  (bases 1 to 9785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5c00095"; journalName: "ACS Synth Biol"; date: 
            "2025-10-17- 17"; volume: "14"; issue: "10"; pages: "3890-3898"
COMMENT     Sequence Label: pQdCas9.luxR(mut)-sggfp
FEATURES             Location/Qualifiers
     source          1..9785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3139..3173
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        3194..3269
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(3348..4010)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGE*QLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      4011..4782
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             6262..7056
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPVEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6316..6335)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6926..6945
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     RBS             8793..8804
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(8811..8837,8838..8840,8841..9785,1..1542,1543..1545,
                     1546..3132)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gatgaacatc atcaagactt gactctttta aaagctttag ttcgacaaca acttccagaa
       61 aagtataaag aaatcttttt tgatcaatca aaaaacggat atgcaggtta tattgatggg
      121 ggagctagcc aagaagaatt ttataaattt atcaaaccaa ttttagaaaa aatggatggt
      181 actgaggaat tattggtgaa actaaatcgt gaagatttgc tgcgcaagca acggaccttt
      241 gacaacggct ctattcccca tcaaattcac ttgggtgagc tgcatgctat tttgagaaga
      301 caagaagact tttatccatt tttaaaagac aatcgtgaga agattgaaaa aatcttgact
      361 tttcgaattc cttattatgt tggtccattg gcgcgtggca atagtcgttt tgcatggatg
      421 actcggaagt ctgaagaaac aattacccca tggaattttg aagaagttgt cgataaaggt
      481 gcttcagctc aatcatttat tgaacgcatg acaaactttg ataaaaatct tccaaatgaa
      541 aaagtactac caaaacatag tttgctttat gagtatttta cggtttataa cgaattgaca
      601 aaggtcaaat atgttactga aggaatgcga aaaccagcat ttctttcagg tgaacagaag
      661 aaagccattg ttgatttact cttcaaaaca aatcgaaaag taaccgttaa gcaattaaaa
      721 gaagattatt tcaaaaaaat agaatgtttt gatagtgttg aaatttcagg agttgaagat
      781 agatttaatg cttcattagg tacctaccat gatttgctaa aaattattaa agataaagat
      841 tttttggata atgaagaaaa tgaagatatc ttagaggata ttgttttaac attgacctta
      901 tttgaagata gggagatgat tgaggaaaga cttaaaacat atgctcacct ctttgatgat
      961 aaggtgatga aacagcttaa acgtcgccgt tatactggtt ggggacgttt gtctcgaaaa
     1021 ttgattaatg gtattaggga taagcaatct ggcaaaacaa tattagattt tttgaaatca
     1081 gatggttttg ccaatcgcaa ttttatgcag ctgatccatg atgatagttt gacatttaaa
     1141 gaagacattc aaaaagcaca agtgtctgga caaggcgata gtttacatga acatattgca
     1201 aatttagctg gtagccctgc tattaaaaaa ggtattttac agactgtaaa agttgttgat
     1261 gaattggtca aagtaatggg gcggcataag ccagaaaata tcgttattga aatggcacgt
     1321 gaaaatcaga caactcaaaa gggccagaaa aattcgcgag agcgtatgaa acgaatcgaa
     1381 gaaggtatca aagaattagg aagtcagatt cttaaagagc atcctgttga aaatactcaa
     1441 ttgcaaaatg aaaagctcta tctctattat ctccaaaatg gaagagacat gtatgtggac
     1501 caagaattag atattaatcg tttaagtgat tatgatgtcg atgccattgt tccacaaagt
     1561 ttccttaaag acgattcaat agacaataag gtcttaacgc gttctgataa aaatcgtggt
     1621 aaatcggata acgttccaag tgaagaagta gtcaaaaaga tgaaaaacta ttggagacaa
     1681 cttctaaacg ccaagttaat cactcaacgt aagtttgata atttaacgaa agctgaacgt
     1741 ggaggtttga gtgaacttga taaagctggt tttatcaaac gccaattggt tgaaactcgc
     1801 caaatcacta agcatgtggc acaaattttg gatagtcgca tgaatactaa atacgatgaa
     1861 aatgataaac ttattcgaga ggttaaagtg attaccttaa aatctaaatt agtttctgac
     1921 ttccgaaaag atttccaatt ctataaagta cgtgagatta acaattacca tcatgcccat
     1981 gatgcgtatc taaatgccgt tgttggaact gctttgatta agaaatatcc aaaacttgaa
     2041 tcggagtttg tctatggtga ttataaagtt tatgatgttc gtaaaatgat tgctaagtct
     2101 gagcaagaaa taggcaaagc aaccgcaaaa tatttctttt actctaatat catgaacttc
     2161 ttcaaaacag aaattacact tgcaaatgga gagattcgca aacgccctct aatcgaaact
     2221 aatggggaaa ctggagaaat tgtctgggat aaagggcgag attttgccac agtgcgcaaa
     2281 gtattgtcca tgccccaagt caatattgtc aagaaaacag aagtacagac aggcggattc
     2341 tccaaggagt caattttacc aaaaagaaat tcggacaagc ttattgctcg taaaaaagac
     2401 tgggatccaa aaaaatatgg tggttttgat agtccaacgg tagcttattc agtcctagtg
     2461 gttgctaagg tggaaaaagg gaaatcgaag aagttaaaat ccgttaaaga gttactaggg
     2521 atcacaatta tggaaagaag ttcctttgaa aaaaatccga ttgacttttt agaagctaaa
     2581 ggatataagg aagttaaaaa agacttaatc attaaactac ctaaatatag tctttttgag
     2641 ttagaaaacg gtcgtaaacg gatgctggct agtgccggag aattacaaaa aggaaatgag
     2701 ctggctctgc caagcaaata tgtgaatttt ttatatttag ctagtcatta tgaaaagttg
     2761 aagggtagtc cagaagataa cgaacaaaaa caattgtttg tggagcagca taagcattat
     2821 ttagatgaga ttattgagca aatcagtgaa ttttctaagc gtgttatttt agcagatgcc
     2881 aatttagata aagttcttag tgcatataac aaacatagag acaaaccaat acgtgaacaa
     2941 gcagaaaata ttattcattt atttacgttg acgaatcttg gagctcccgc tgcttttaaa
     3001 tattttgata caacaattga tcgtaaacga tatacgtcta caaaagaagt tttagatgcc
     3061 actcttatcc atcaatccat cactggtctt tatgaaacac gcattgattt gagtcagcta
     3121 ggaggtgact aactcgagtt gacagctagc tcagtcctag gtataatact agttgctcga
     3181 gccctggaag tacgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     3241 aacttgaaaa agtggcaccg agtcggtgct ttttttgtta acttcaggac ctgcagtcta
     3301 gtatagtctg gaacagcgca cttacgggtt gctgcgcaac ccaagtgcta ccggcgcggc
     3361 agcgtgaccc gtgtcggcgg ctccaacggc tcgccatcgt ccagaaaaca cggctcatcg
     3421 ggcatcggca ggcgctgctg cccgcgccgt tcccattcct ccgtttcggt caaggctggc
     3481 aggtctggtt ccatgcccgg aatgccgggc tggctgggcg gctcctcgcc ggggccggtc
     3541 ggtagttgct actcgcccgg atacagggtc gggatgcggc gcaggtcgcc atgccccaac
     3601 agcgattcgt cctggtcgtc gtgatcaacc accacggcgg cactgaacac cgacaggcgc
     3661 aactggtcgc ggggctggcc ccacgccacg cggtcattga ccacgtaggc cgacacggtg
     3721 ccggggccgt tgagcttcac gacggagatc cagcgctcgg ccaccaagtc cttgactgcg
     3781 tattggaccg tccgcaaaga acgtccgatg agcttggaaa gtgtcttctg gctgaccacc
     3841 acggcgttct ggtggcccat ctgcgccacg aggtgatgca gcagcattgc cgccgtgggt
     3901 ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc gttccgtttg cacccagtga
     3961 ccgggcttgt tcttggcttg aatgccgatt tctctggact gcgtggccat gcttatctcc
     4021 atgcggtagg ggtgccgcac ggttgcggca ccatgcacaa tcagctgcaa ctttttggca
     4081 gcgcgacaac aattatgcgt tgcgtaaaag tggcagtcaa ttacagattt tctttaacct
     4141 acgcaatgag ctattgcggg gggtgccgca atgagctgtt gcgtaccccc cttttttaag
     4201 ttgttgattt ttaagtcttt cgcatttcgc cctatatcta gttctttggt gcccaaagaa
     4261 gggcacccct gcggggttcc cccacgcctt cggcgcggct ccccctccgg caaaaagtgg
     4321 cccctccggg gcttgttgat cgactgcgcg gccttcggcc ttgcccaagg tggcgctgcc
     4381 cccttggaac ccccgcactc gccgccgtga ggctcggggg gcaggcgggc gggcttcgcc
     4441 cttcgactgc ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc
     4501 tgcgcggtcg ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa
     4561 gcgcgcaggc cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca
     4621 aggccgcagg ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg
     4681 ggcaatccct gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct tgtccagcag
     4741 ggttgtccac gggccgagcg aagcgagcca gccggtggcc gctcgcggcc atcgtccaca
     4801 tatccacggg ctggcaaggg agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc
     4861 cgtagggcgc cgcagccgcc gtaggcggtc acgactttgc gaagcaaagt ctagtgagta
     4921 tactcaagca ttgagtggcc cgccggaggc accgccttgc gctgcccccg tcgagccggt
     4981 tggacaccaa aagggagggg caggcatggc ggcatacgcg atcatgcgat gcaagaagct
     5041 ggcgaaaatg ggcaacgtgg cggccagtct caagcacgcc taccgcgagc gcgagacgcc
     5101 caacgctgac gccagcagga cgccagagaa cgagcactgg gcggccagca gcaccgatga
     5161 agcgatgggc cgactgcgcg agttgctgcc agagaagcgg cgcaaggacg ctgtgttggc
     5221 ggtcgagtac gtcatgacgg ccagcccgga atggtggaag tcggccagcc aagaacagca
     5281 ggcggcgttc ttcgagaagg cgcacaagtg gctggcggac aagtacgggg cggatcgcat
     5341 cgtgacggcc agcatccacc gtgacgaaac cagcccgcac atgaccgcgt tcgtggtgcc
     5401 gctgacgcag gacggcaggc tgtcggccaa ggagttcatc ggcaacaaag cgcagatgac
     5461 ccgcgaccag accacgtttg cggctgctgt ggccgatcta gggctgcaac ggggcatcga
     5521 gggcagcaag gcacgtcaca cgcgcattca ggcgttctac gaggccctgg agcggccacc
     5581 agtgggccac gtcaccatca gcccgcaagc ggtcgagcca cgcgcctatg caccgcaggg
     5641 attggccgaa aagctgggaa tctcaaagcg cgttgagacg ccggaagccg tggccgaccg
     5701 gctgacaaaa gcggttcggc aggggtatga gcctgcccta caggccgccg caggagcgcg
     5761 tgagatgcgc aagaaggccg atcaagccca agagacggcc cgagaccttc gggagcgcct
     5821 gaagcccgtt ctggacgccc tggggccgtt gaatcgggat atgcaggcca aggccgccgc
     5881 gatcatcaag gccgtgggcg aaaagctgct gacggaacag cgggaagtcc agcgccagaa
     5941 acaggcccag cgccagtagg aacgcgggcg cgcacatttc cccgaaaagt gccacctggg
     6001 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
     6061 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
     6121 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
     6181 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatt tgatcaagag
     6241 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     6301 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     6361 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     6421 tccggtgccc tgaatgaact acaggacgag gcagcgcggc tatcgtggct ggccacgacg
     6481 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     6541 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgt cgagaaagta
     6601 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     6661 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     6721 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6781 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     6841 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6901 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6961 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     7021 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     7081 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     7141 aaaggttgga ctagagaatt catcctgaac ttatctagat taatttaaga ctgctttttt
     7201 aaactgttca ttaataggca tagacaatac aaccgattta gtatcaccta atacatgaat
     7261 ttctttgtct ccaatacgat gacaaggaac tttaatacgc tttaaaaatc gctctattgc
     7321 tgttgatgtt actgttacat attctgtaat accttgacta acagcgtgtt tatatatagc
     7381 ttcaaatagt ttcattgtaa tttcactagc agagttattt atctttgagc tatttttacc
     7441 tacagcaaaa cgacttaatt cgactatatt aggatctttg ggagcactct gttgaccaag
     7501 caattcagga aaaacacttt tcagcatata atcacctgtt gtaggtaata aacgccagca
     7561 tccacttaca ttttcagtat catcacaagc ataaatatat tctgcatttg agttatcata
     7621 ctcatctgat tcaaggttat tttctacaac taagtcccac tcaagtcttt gcttaaacac
     7681 ttgataacga agacttagaa tacctttata ctcctccgat ggaattgcca aaaaatccga
     7741 tttttttatc attatagtca taccaacctc ccttgcgttt attcgactat aacaaaccat
     7801 tttcttgcgt aaacctgtac gatcctacag gtgcttatgt taagtaattg tattcccagc
     7861 gatacaatag tgtgacaaaa atccaattta ttagaatcaa atgtcaatcc attaccgttt
     7921 taatgatata taacacgcaa aacttgcgac aaacaatagg taaggataaa gagatgggta
     7981 tgaaaaacat aaatgccgac gacacataca gaataattaa taaaattaaa gcttgtggca
     8041 gcaataatga tattaatcaa tgcttatctg atatgactaa aatggtacat tgtgaatatt
     8101 atttactcgc gatcatttat cctcattcta tggttaaatc tgatatttca attctagata
     8161 attaccctaa aaaatggagg caatattatg gcgcgctaat ttaataaaat atgatcctat
     8221 agtagattat tctaaatcca atcattcacc aattaattgg aatatatttg aaaacaatgc
     8281 tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa acatcaggtc ttatcactgg
     8341 gtttagtttc cctattcata cggctaacaa tggcttcgga atgcttagtt ttgcacattc
     8401 agaaattgac aactatatag atagtttatt tttacatgcg tgtatgaaca taccattaat
     8461 tgttccttct ctagttgata attatcgaaa aataaatata gcaaataata aatcaaacaa
     8521 cgatttaacc aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa aaagctcttg
     8581 ggatatttca aaaatattag gctgcagtga gcgtactgtc actttccatt taaccaatgc
     8641 gcaaatgaaa ctcgatacaa caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg
     8701 agcaattgat tggccatttt ttaaaaatta aagatctacc tgtaggatcg tacaggttta
     8761 cgcaagaaaa tggtttgtta tagtcgaata aaaaagagga gaaaggatct atggataaga
     8821 aatactcaat aggcttagct atcggcacaa atagcgtcgg atgggcggtg atcactgatg
     8881 aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca
     8941 aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc
     9001 tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg
     9061 agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt
     9121 cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag
     9181 atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag
     9241 attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt
     9301 ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac
     9361 tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa
     9421 gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa
     9481 atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt
     9541 tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat
     9601 tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg caaattggag
     9661 atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag
     9721 atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac
     9781 gctac
//
