LOCUS       sequence-471056-        4825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2575m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP20139 - pAAV-AiE2575m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      761..770
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             773..793
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1859..1970
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2088..2228
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2088..2217
                     /label=AAV2 ITR
     rep_origin      2303..2758
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2390..2409)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2600..2621
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2775..2794)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2894..2916
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2954..2972)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3040..3144
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3145..3213,3214..4005)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3363..3382)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4176..4764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4665..4684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcat tactgcagtc acaccgaagg gaagattcaa cgagaatggg
      181 aaattatgag aaatgcccac acttcctatt tcctatatct attttagttg gtggcaatta
      241 caatttacta cgcaacactc aaaatataaa cggcactatg tgaaaggata taacagtgac
      301 agatttgcag cctcaaagcg tgacttcaaa atattggtgc cgaaaaggtt tcctgttccg
      361 gaaggcagtt gagactacaa agcccacgca ggtgaaggcg ctggcaggag cgagtgctct
      421 gcgtaccctg gatgttgagc aaagctgtgc agggtagatg ggaggcgccg cccggcccca
      481 tatggagccg accttccgtg ccaatcgcac tatgctatgg cacacacagc gagctgagcg
      541 aggaaatcct gcgaagcagg gaaactgaga gcataaggcc cggagttgaa aatcaagaag
      601 gaaagccaag agtttgaggg aaatgcagag gcagggttat gtgaggagaa catgccagtg
      661 tcctgtctct ttagccccgg agctcgggct gggcataaaa gtcagggcag agccatctat
      721 tgcttacatt tgcttctggg atccagatct ttcgaagcta gccaccatgg tgcccaagaa
      781 gaagaggaaa gtctccaacc tgctgactgt gcaccaaaac ctgcctgccc tccctgtgga
      841 tgccacctct gatgaagtca ggaagaacct gatggacatg ttcagggaca ggcaggcctt
      901 ctctgaacac acctggaaga tgctcctgtc tgtgtgcaga tcctgggctg cctggtgcaa
      961 gctgaacaac aggaaatggt tccctgctga acctgaggat gtgagggact acctcctgta
     1021 cctgcaagcc agaggcctgg ctgtgaagac catccaacag cacctgggcc agctcaacat
     1081 gctgcacagg agatctggcc tgcctcgccc ttctgactcc aatgctgtgt ccctggtgat
     1141 gaggagaatc agaaaggaga atgtggatgc tggggagaga gccaagcagg ccctggcctt
     1201 tgaacgcact gactttgacc aagtcagatc cctgatggag aactctgaca gatgccagga
     1261 catcaggaac ctggccttcc tgggcattgc ctacaacacc ctgctgcgca ttgccgaaat
     1321 tgccagaatc agagtgaagg acatctcccg caccgatggt gggagaatgc tgatccacat
     1381 tggcaggacc aagaccctgg tgtccacagc tggtgtggag aaggccctgt ccctgggggt
     1441 taccaagctg gtggagagat ggatctctgt gtctggtgtg gctgatgacc ccaacaacta
     1501 cctgttctgc cgggtcagaa agaatggtgt ggctgcccct tctgccacct cccaactgtc
     1561 cacccgggcc ctggaaggga tctttgaggc cacccaccgc ctgatctatg gtgccaagga
     1621 tgactctggg cagagatacc tggcctggtc tggccactct gccagagtgg gtgctgccac
     1681 cgacatggcc agggctggtg tgtccatccc tgaaatcatg caggctggtg gctggaccaa
     1741 tgtgaacatt gtgatgaact acatcagaaa cctggactct gagactgggg ccatggtgag
     1801 gctgctcgaa gatggggact gaggcgcgcc gaattcaagc ttctcgagag atcttcgact
     1861 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     1921 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     1981 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     2041 gaagacaata gcaggcatga gatctcacgt gcggaccgag cggccgcagg aacccctagt
     2101 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     2161 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     2221 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     2281 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     2341 gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct
     2401 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     2461 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     2521 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     2581 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc
     2641 tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat
     2701 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     2761 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     2821 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     2881 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     2941 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     3001 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3061 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3121 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3181 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3241 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3301 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3361 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3421 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3481 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3541 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3601 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3661 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3721 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3781 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3841 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     3901 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3961 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4021 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4081 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4141 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4201 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4261 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4321 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4381 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4441 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4501 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4561 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4621 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4681 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4741 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4801 ttttgctggc cttttgctca catgt
//
