LOCUS       sequence-471057-        4848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0589m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP20140 - pAAV-AiE0589m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      784..793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             796..816
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1882..1993
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2111..2251
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2111..2240
                     /label=AAV2 ITR
     rep_origin      2326..2781
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2413..2432)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2623..2644
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2798..2817)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2917..2939
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2977..2995)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3063..3167
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3168..3236,3237..4028)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3386..3405)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4199..4787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4688..4707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttggaag gagatgcatt tgtattgaaa ttttctgttt
      181 catttaaagt ttgttttttt ctttaaatac tgggatatat tttcttcctg tacagccttg
      241 cctccttcca atttatgttc aggataaaga ttacatttag gccatttata aatggttaga
      301 gtcaatcaac acggtaaaca ttcgtatagc aattattgca aattaaccac ttggatgtta
      361 atcatttctg tttgtgcttt ttttttttcc cctctaatgt tgatttctga ggcctttaaa
      421 gatggtaact ttattatcct tataagatgc caacatttga gtcatcaaca ttggttaaaa
      481 ataaaggggg atcaccttga gctgcagaca tgcaaatcaa ctggaaaaac accagctggc
      541 cttaatcttt caccattgca gttttgtctg cctcctgtca gcaggctagc aggtaggctt
      601 tttatagaaa cagggactta gcagatgtag ctgctgtttc cttcttgtca acactgaaat
      661 tctccttttt agcccctctg acacacacag acaccaaccg aagagctcgg gctgggcata
      721 aaagtcaggg cagagccatc tattgcttac atttgcttct gggatccaga tctttcgaag
      781 ctagccacca tggtgcccaa gaagaagagg aaagtctcca acctgctgac tgtgcaccaa
      841 aacctgcctg ccctccctgt ggatgccacc tctgatgaag tcaggaagaa cctgatggac
      901 atgttcaggg acaggcaggc cttctctgaa cacacctgga agatgctcct gtctgtgtgc
      961 agatcctggg ctgcctggtg caagctgaac aacaggaaat ggttccctgc tgaacctgag
     1021 gatgtgaggg actacctcct gtacctgcaa gccagaggcc tggctgtgaa gaccatccaa
     1081 cagcacctgg gccagctcaa catgctgcac aggagatctg gcctgcctcg cccttctgac
     1141 tccaatgctg tgtccctggt gatgaggaga atcagaaagg agaatgtgga tgctggggag
     1201 agagccaagc aggccctggc ctttgaacgc actgactttg accaagtcag atccctgatg
     1261 gagaactctg acagatgcca ggacatcagg aacctggcct tcctgggcat tgcctacaac
     1321 accctgctgc gcattgccga aattgccaga atcagagtga aggacatctc ccgcaccgat
     1381 ggtgggagaa tgctgatcca cattggcagg accaagaccc tggtgtccac agctggtgtg
     1441 gagaaggccc tgtccctggg ggttaccaag ctggtggaga gatggatctc tgtgtctggt
     1501 gtggctgatg accccaacaa ctacctgttc tgccgggtca gaaagaatgg tgtggctgcc
     1561 ccttctgcca cctcccaact gtccacccgg gccctggaag ggatctttga ggccacccac
     1621 cgcctgatct atggtgccaa ggatgactct gggcagagat acctggcctg gtctggccac
     1681 tctgccagag tgggtgctgc caccgacatg gccagggctg gtgtgtccat ccctgaaatc
     1741 atgcaggctg gtggctggac caatgtgaac attgtgatga actacatcag aaacctggac
     1801 tctgagactg gggccatggt gaggctgctc gaagatgggg actgaggcgc gccgaattca
     1861 agcttctcga gagatcttcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     1921 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     1981 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     2041 aggacagcaa gggggaggat tgggaagaca atagcaggca tgagatctca cgtgcggacc
     2101 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     2161 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     2221 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     2281 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     2341 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2401 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2461 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2521 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     2581 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2641 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
     2701 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2761 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     2821 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2881 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2941 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     3001 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3061 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3121 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3181 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3241 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3301 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3361 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3421 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3481 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3541 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3601 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3661 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3721 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3781 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3841 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3901 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3961 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4021 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4081 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4141 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4201 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4261 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4321 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4381 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4441 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4501 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4561 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4621 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4681 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4741 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4801 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
