LOCUS       sequence-471096-        8806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinant protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Sung YC, Li Y, Bernasconi Z, Baik S, Asuke S, Keller B, Fahima T, 
            Coaker G
  TITLE     Wheat tandem kinase RWT4 directly binds a fungal effector to 
            activate defense.
  JOURNAL   Nat Genet. 2025 Apr 14. doi: 10.1038/s41588-025-02162-w.
  PUBMED    40229601
REFERENCE   2  (bases 1 to 8806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 Apr 14. doi: 10.1038/s41588-025-02162-w."
COMMENT     Sequence Label: pET28a:His-MBP-RWT4Ca_noKDup
FEATURES             Location/Qualifiers
     source          1..8806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2016..3098)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3060..3079
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3099..3176)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3382..3401
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     3485..3504
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3485..3503
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3504..3528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3543..3565
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3584..3601
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3611..4711
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     4685..4708
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             4766..4786
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             7154..7171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7224..7242)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7238..7285
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7322..7777
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7409..7428)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7619..7640
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7870..8685)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7870..8685)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8593..8612
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8677..8696
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat accctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     3481 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     3541 attttgttta actttaagaa ggagatatac catgggttct tctcaccatc accatcacca
     3601 tggttcttct atgaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg
     3661 ctataacggt ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac
     3721 cgttgagcat ccggataaac tggaagagaa attcccacag gttgcggcaa ctggcgatgg
     3781 ccctgacatt atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt
     3841 ggctgaaatc accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc
     3901 cgtacgttac aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat
     3961 ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga
     4021 taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt
     4081 cacctggccg ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta
     4141 cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt
     4201 tgacctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc
     4261 ctttaataaa ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga
     4321 caccagcaaa gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa
     4381 accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc
     4441 aaaagagttc ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga
     4501 caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg
     4561 tattgccgcc actatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat
     4621 gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac
     4681 tgtcgatgaa gccctgaaag acgcgcagac taattcgagc tcgaacaaca acaacaataa
     4741 caataacaac aacctcggga tcgaggaaaa cctgtacttc caatccaatg ccatggacgg
     4801 ctacgagttc cagcgtgctg agcttgatgc attggagaga gttgttagag atacctctgc
     4861 cgagccaatg tccttgacac tgcccctttt gcgtcatatc acaaatgatt tctcagatga
     4921 atccaggatc ggacgtggag gattcgctgt tgtctatctt ggtgttttgc catccggcct
     4981 gagaatagct gttaagaggt tgtcaaatat agcttatatg aatgaaagtg cattccagaa
     5041 cgaagtattc atcacaatga aagccacaca taaaaataca gtgagattca tgggatactg
     5101 ctcacagatt caaggcaaac taatagaaca tgatggacaa catgtctttg cacagcttga
     5161 agagaggcta atttgtgtgg aatacgcacc taagggaaca ttagacgctc acatcggaga
     5221 ttatggtgaa ctggactgga accaaagata tcagattctg aaaggcatat gtcagggttt
     5281 acatcatcta catgacgaaa tgcatgtctt tcatggagat atcaaacccg ccaacattct
     5341 tatcggcgat aacctagttc ctaaaatcta tgatttcgga ttgagtcaaa tgttcgagga
     5401 ggaggaaacg gaaagaattg tggaaaacat tgccggtaca ttcggctaca tggcacctga
     5461 gttctgtaca aacaacatgg tatccttcaa ggccgagatt tacagtttag gcgttgtaat
     5521 aggagaactt cttatcggta aaaaaggatg gtttgacgag gacgtgcgga agctttttgt
     5581 tcagcagttg aaaggccttc gcaaaacttt ggtgaaggaa ggcgcgtttt ccagctggga
     5641 gaataaatac catcaagtgc gcacgtgtat ggagattggt caggattgca tcgatccaaa
     5701 tcctcataaa cggcctacgc ttctcgaaat tattcaacgc cttaacgaag ccgaagacat
     5761 gaattatagt gcggcatcac tctggcaggt tcagtctggt gatgaagaat cagatctctc
     5821 cgatactgaa gcgttggaga cagagaccac gagcgagttt cttccgtccg atgaagaacc
     5881 ggcgtccgtc ggtaagaccg gcgagacctc tacacaggaa ccggacaaac ccgatcttat
     5941 atctaaactt cccgcttcgg ttgacctttc tgatctcaag gtgcttgaaa agataaccga
     6001 tgattttagc catgagagaa tagtcgggaa agacgggact tttaaaggat gccataaggc
     6061 tttcgtttat aaaggtgaca ttcctctgag ggagatgatt gcagttaaac ggctcattgg
     6121 tgttgagata cccttcgaga agttcaagag agaggctgaa caattcatca gtttggatca
     6181 taagaacata gttaaggttg caagttattg ccacgaccag agccggggac atagactcgt
     6241 ccagttcaag gggaagcctc tgccccaact ttttaatggg cctgagcaac tgttgtgtta
     6301 cgagtacatg cacaatggtt ctcttagaga ttacttgatg ggccaaggct cccgggtcat
     6361 agattggcag atgcggtaca agctgataaa agggacctgc gcaggcttgc attatcttca
     6421 taagggtcgc gcaggatgtc cgatcgttca ccttaatctg agtcccagca acgtgctttt
     6481 ggatcataat tacattccgc ggataactgg cttcgacttt tccaagctta taggagaaaa
     6541 aaacactaaa tctgtggtct tgaagctcaa tggacccatc gcttatctgc cccccgactt
     6601 cttccacagc aaaggtacag atctcaagta cctggcgaca gttgatatat actctttggg
     6661 cctgatgata ctcgagattg caacacagca ggaaatcaag ggcatacatg gtgttctgat
     6721 aaagtcaatc gaggaaaact ggcgggaaga gtcccaaatc acacgcctct atacctctct
     6781 tggggctgat gagctgaggc aggtgaagat gtgtattgat ataggacttg actgtgtcaa
     6841 gagtaaccca gagaagcgcc ccactgcagg agcaattatg ttgtggcttg ataaagaatc
     6901 taagccagtc cccgtgtcac gggccggagc gggcgttttg ccacggcccc cagttccgac
     6961 caacatcaac catgcggggc gcatccaggg tatcccaatc gagaaatcac aagtccttat
     7021 tccttcattc gcttgtaatg aatatagctc tgtcaaaatt caaaatttgc acttgctgct
     7081 ctttatgtaa cattggaagt ggataacgga tccgaattcg agcgccgtcg acaagcttgc
     7141 ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
     7201 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     7261 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
     7321 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7381 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7441 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7501 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7561 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7621 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7681 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7741 aacgcgaatt ttaacaaact agtaacgttt acaatttcag gtggcacttt tcggggaaat
     7801 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7861 aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     7921 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     7981 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     8041 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     8101 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
     8161 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     8221 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
     8281 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatgt tttcacctga
     8341 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
     8401 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     8461 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     8521 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
     8581 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     8641 taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
     8701 actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt
     8761 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
