LOCUS       sequence-471102-       14937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14937)
  AUTHORS   Sung YC, Li Y, Bernasconi Z, Baik S, Asuke S, Keller B, Fahima T, 
            Coaker G
  TITLE     Wheat tandem kinase RWT4 directly binds a fungal effector to 
            activate defense.
  JOURNAL   Nat Genet. 2025 Apr 14. doi: 10.1038/s41588-025-02162-w.
  PUBMED    40229601
REFERENCE   2  (bases 1 to 14937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 Apr 14. doi: 10.1038/s41588-025-02162-w."
COMMENT     Sequence Label: pTA7001-RWT4Ca_KDup
FEATURES             Location/Qualifiers
     source          1..14937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..642
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  1335..2102
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             2294..3088
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3387..4535
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6131..6155
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(6402..6423)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6614..6633
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6758..6780
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6772..6789
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6773..6789
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6999..7045
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    7077..7101
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7557..7622
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(7626..7650)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7658..7723
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      complement(8249..8501)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(9616..9799)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(9652..9831)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(11123..11356)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        complement(11618..11963)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    12478..12502
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12967..13617
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(13805..13822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(14056..14075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            14298..14407
                     /note="incP origin of transfer"
     CDS             14440..14811
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 gcgcctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agcgctggca
      721 gtccttgcca ttgccgggat cggggcagta acgggatggg cgatcagccc gagcgcgacg
      781 cccggaagca ttgacgtgcc gcaggtgctg gcatcgacat tcagcgacca ggtgccgggc
      841 agtgagggcg gcggcctggg tggcggcctg cccttcactt cggccgtcgg ggcattcacg
      901 gacttcatgg cggggccggc aatttttacc ttgggcattc ttggcatagt ggtcgcgggt
      961 gccgtgctcg tgttcggggg tgcgataaac ccagcgaacc atttgaggtg ataggtaaga
     1021 ttataccgag gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat
     1081 atttaaaaag ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat
     1141 atattgacaa tactgataag ataatatatc ttttatatag aagatatcgc cgtatgtaag
     1201 gatttcaggg ggcaaggcat aggcagcgcg cttatcaata tatctataga atgggcaaag
     1261 cataaaaact tgcatggact aatgcttgaa acccaggaca ataaccttat agcttgtaaa
     1321 ttctatcata attgggtaat gactccaact tattgatagt gttttatgtt cagataatgc
     1381 ccgatgactt tgtcatgcag ctccaccgat tttgagaacg acagcgactt ccgtcccagc
     1441 cgtgccaggt gctgcctcag attcaggtta tgccgctcaa ttcgctgcgt atatcgcttg
     1501 ctgattacgt gcagctttcc cttcaggcgg gattcataca gcggccagcc atccgtcatc
     1561 catatcacca cgtcaaaggg tgacagcagg ctcataagac gccccagcgt cgccatagtg
     1621 cgttcaccga atacgtgcgc aacaaccgtc ttccggagac tgtcatacgc gtaaaacagc
     1681 cagcgctggc gcgatttagc cccgacatag ccccactgtt cgtccatttc cgcgcagacg
     1741 atgacgtcac tgcccggctg tatgcgcgag gttaccgact gcggcctgag ttttttaagt
     1801 gacgtaaaat cgtgttgagg ccaacgccca taatgcgggc tgttgcccgg catccaacgc
     1861 cattcatggc catatcaatg attttctggt gcgtaccggg ttgagaagcg gtgtaagtga
     1921 actgcagttg ccatgtttta cggcagtgag agcagagata gcgctgatgt ccggcggtgc
     1981 ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga gggacagctg atagaaacag
     2041 aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca
     2101 cccataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     2161 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     2221 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     2281 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     2341 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     2401 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     2461 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     2521 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     2581 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     2641 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     2701 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     2761 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     2821 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     2881 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     2941 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     3001 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     3061 ttatatttta ctggatgaat tgttttagta cctagatgtg gcgcaacgat gccggcgaca
     3121 agcaggagcg caccgacttc ttccgcatca agtgttttgg ctctcaggcc gaggcccacg
     3181 gcaagtattt gggcaagggg tcgctggtat tcgtgcaggg caagattcgg aataccaagt
     3241 acgagaagga cggccagacg gtctacggga ccgacttcat tgccgataag gtggattatc
     3301 tggacaccaa ggcaccaggc gggtcaaatc aggaataagg gcacattgcc ccggcgtgag
     3361 tcggggcaat cccgcaagga gggtgaatga atcggacgtt tgaccggaag gcatacaggc
     3421 aagaactgat cgacgcgggg ttttccgccg aggatgccga aaccatcgca agccgcaccg
     3481 tcatgcgtgc gccccgcgaa accttccagt ccgtcggctc gatggtccag caagctacgg
     3541 ccaagatcga gcgcgacagc gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg
     3601 ccgtggagcg ttcgcgtcgt ctcgaacagg aggcggcagg tttggcgaag tcgatgacca
     3661 tcgacacgcg aggaactatg acgaccaaga agcgaaaaac cgccggcgag gacctggcaa
     3721 aacaggtcag cgaggccaag caggccgcgt tgctgaaaca cacgaagcag cagatcaagg
     3781 aaatgcagct ttccttgttc gatattgcgc cgtggccgga cacgatgcga gcgatgccaa
     3841 acgacacggc ccgctctgcc ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc
     3901 tgcaaaacaa ggtcattttc cacgtcaaca aggacgtgaa gatcacctac accggcgtcg
     3961 agctgcgggc cgacgatgac gaactggtgt ggcagcaggt gttggagtac gcgaagcgca
     4021 cccctatcgg cgagccgatc accttcacgt tctacgagct ttgccaggac ctgggctggt
     4081 cgatcaatgg ccggtattac acgaaggccg aggaatgcct gtcgcgccta caggcgacgg
     4141 cgatgggctt cacgtccgac cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct
     4201 tccgcgtcct ggaccgtggc aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa
     4261 tcgtcgtgct gtttgctggc gaccactaca cgaaattcat atgggagaag taccgcaagc
     4321 tgtcgccgac ggcccgacgg atgttcgact atttcagctc gcaccgggag ccgtacccgc
     4381 tcaagctgga aaccttccgc ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc
     4441 gcgagcaggt cggcgaagcc tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct
     4501 gggtcaatga tgacctggtg cattgcaaac gctagggcct tgtggggtca gttccggctg
     4561 ggggttcagc agccagcgct ttactggcat ttcaggaaca agcgggcact gctcgacgca
     4621 cttgcttcgc tcagtatcgc tcgggacgca cggcgcgctc tacgaactgc cgataaacag
     4681 aggattaaaa ttgacaattg tgattaaggc tcagattcga cggcttggag cggccgacgt
     4741 gcaggatttc cgcgagatcc gattgtcggc cctgaagaaa gctccagaga tgttcgggtc
     4801 cgtttacgag cacgaggaga aaaagcccat ggaggcgttc gctgaacggt tgcgagatgc
     4861 cgtggcattc ggcgcctaca tcgacggcga gatcattggg ctgtcggtct tcaaacagga
     4921 ggacggcccc aaggacgctc acaaggcgca tctgtccggc gttttcgtgg agcccgaaca
     4981 gcgaggccga ggggtcgccg gtatgctgct gcgggcgttg ccggcgggtt tattgctcgt
     5041 gatgatcgtc cgacagattc caacgggaat ctggtggatg cgcatcttca tcctcggcgc
     5101 acttaatatt tcgctattct ggagcttgtt gtttatttcg gtctaccgcc tgccgggcgg
     5161 ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc gtgttcatct ctgccgctct
     5221 gctaggtagc ccgatacgat tgatggcggt cctgggggct atttgcggaa ctgcgggcgt
     5281 ggcgctgttg gtgttgacac caaacgcagc gctagatcct gtcggcgtcg cagcgggcct
     5341 ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc cgcaagtggc aacctcccgt
     5401 gcctctgctc acctttaccg cctggcaact ggcggccgga ggacttctgc tcgttccagt
     5461 agctttagtg tttgatccgc caatcccgat gcctacagga accaatgttc tcggcctggc
     5521 gtggctcggc ctgatcggag cgggtttaac ctacttcctt tggttccggg ggatctcgcg
     5581 actcgaacct acagttgttt ccttactggg ctttctcagc cccagatctg gggtcgatca
     5641 gccggggatg catcaggccg acagtcggaa cttcgggtcc ccgacctgta ccattcggtg
     5701 agcaatggat aggggagttg atatcgtcaa cgttcacttc taaagaaata gcgccactca
     5761 gcttcctcag cggctttatc cagcgatttc ctattatgtc ggcatagttc tcaagatcga
     5821 cagcctgtca cggttaagcg agaaatgaat aagaaggctg ataattcgga tctctgcgag
     5881 ggagatgata tttgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
     5941 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
     6001 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
     6061 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
     6121 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
     6181 acacattgcg gacgttttta atgtactggg gtggtttttc ttttcaccag tgagacgggc
     6241 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     6301 gtttgcccca gcaggcgaaa atcctgtttg atggtggttc cgaaatcggc aaaatccctt
     6361 ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     6421 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6481 gcccactacg tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6541 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6601 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc cattcaggct gcgcaactgt
     6661 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     6721 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     6781 acggccagtg aattcctcga agcttgcatg ccggtcgact ctagaggatc cgggtgacag
     6841 ccctccgacg ggtgacagcc ctccgacggg tgacagccct ccgaattcta gaggatccgg
     6901 gtgacagccc tccgacgggt gacagccctc cgacgggtga cagccctccg aattcgagct
     6961 cggtacccgg ggatctgtcg acctcgatcg agatcttcgc aagacccttc ctctatataa
     7021 ggaagttcat ttcatttgga gaggacacgc tgaagctagt cgactctagc ctcgagacaa
     7081 gtttgtacaa aaaagcaggc tccaccatga tgggtggcta tgagttccag agggcagaat
     7141 tggacgccct tgagggtgta gttcgttacc caaccgccga acccatgtct ttaacactac
     7201 ctcttttgcg tcacattacg aatgatttct cccctgagtt cgagatatct aaggacgact
     7261 ccgccgtggt atatctagga gtgctaccat caggatttag ggtggcagtg aagaaatcac
     7321 attttaggtt ttgtctggac gacgaggacg ccttcactaa tgaagtttca attgcaatga
     7381 aagccgcaca taagaatact gtaagagtaa tcggctattg tcatcataca catgagcaga
     7441 ttgccgagta tgagggaaag caagtcttcg cagaagtgcg tgagaggttg atatgtactg
     7501 agtacgtgcc caatggacct ctatccggcc atatcttgct gctctttatg ggtagcgact
     7561 acaaggacca cgacggtgac tacaaggacc acgacatcga ctacaaggac gacgacgaca
     7621 agatcaccca gctttcttgt acaaagtggt gctcgacgat tataaggatc atgatggaga
     7681 ttataaggat catgatattg attataagga tgatgatgat aagtaaacta gtcgatccag
     7741 gcctcccagc tttcgtccgt atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca
     7801 gtttcattgc ccacacacca gaatcctact aagtttgagt attatggcat tggaaaagct
     7861 gttttcttct atcatttgtt ctgcttgtaa tttactgtgt tctttcagtt tttgttttcg
     7921 gacatcaaaa tgcaaatgga tggataagag ttaataaatg atatggtcct tttgttcatt
     7981 ctcaaattat tattatctgt tgtttttact ttaatgggtt gaatttaagt aagaaaggaa
     8041 ctaacagtgt gatattaagg tgcaatgtta gacatataaa acagtctttc acctctcttt
     8101 ggttatgtct tgaattggtt tgtttcttca cttatctgtg taatcaagtt tactatgagt
     8161 ctatgatcaa gtaattatgc aatcaagtta agtacagtat aggctttttg tgtcgagggg
     8221 gtaccgagtc gaggggggat caattcccga tctagtaaca tagatgacac cgcgcgcgat
     8281 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     8341 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     8401 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     8461 ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggaaatt cgggggatca
     8521 gcttgtgatc atgttacatc acctccaaga tcccggacga gtgctggggc gtcggtttcc
     8581 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
     8641 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
     8701 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
     8761 gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa
     8821 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
     8881 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc
     8941 attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg
     9001 gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
     9061 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
     9121 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
     9181 gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg
     9241 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata
     9301 ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
     9361 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
     9421 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
     9481 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
     9541 cgcggtgagt tcaggctttt tcatatctca ttgccccccg ggatcttgca ggtcgagatc
     9601 ctctagcgga tccggtgcag attatttgga ttgagagtga atatgagact ctaattggat
     9661 accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg
     9721 atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct
     9781 cattaaactc cagaaacccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc
     9841 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
     9901 ctccgctcat gatcagattg tcgtttcccg ccttcagttt caagcttgtt tggggatcta
     9961 gtgttttact cctcatatta acttcggtca ttagaggcca cgatttgaca catttttact
    10021 caaaacaaaa tgtttgcata tctcttataa tttcaaattc aacacacaac aaataagaga
    10081 aaaaacaaat aatattaatt tgagaatgaa caaaaggacc atatcattca ttaactcttc
    10141 tccatccatt tccatttcac agttcgatag cgaaaaccga ataaaaaaca cagtaaatta
    10201 caagcacaac aaatggtaca agaaaaacag ttttcccaat gccataatac tcgaacaagc
    10261 tagcttactc agttaggtcg agtcattttt gatgaaacag aagctttttg atatttccat
    10321 ttgaatattt tggtatctga ttagtgatga tttcagctaa catctctggg aattcaatac
    10381 tcatggtctt atccaaaaat gtctggaagc agtaggtaag gagattctca accacctcat
    10441 gcatggagtc cagaagcttt gtcagttggt aaaaccgttg ccagttctga ctggagttcc
    10501 cttccctttt gacgatggct tttcctagct ctttgatata agtcattcga atctcatcaa
    10561 ataactcttg gctcttcaga ccttccttag gaactgagga gagaagcagt aaggttttca
    10621 tacagagata ctcttcatag gatacctgca atctttgtaa ttcagaggag acaaacagca
    10681 tgtgtttaca ttggtcatac atgcagggta gagacattct ctgctcatta ataatcagat
    10741 caggagcaaa gcagagcagg tttccacttg attgtctgta tgatctccaa cccagggcaa
    10801 atgccatgag aaacatccat gagtactgta gcagggtcat ttggtcatcc aggtgtaagt
    10861 ttctgaagcc tggtatcgcc tttgcccatt tcactgctgc aatcacttga cgcccaccta
    10921 acatgttgag tgtggtcata attctccatg ctgaatctgg aacagagcta tcatatcctg
    10981 catacaacac ctcgggttca atcacctcca gcagtgacac caaggtaggg gtgagctgtg
    11041 gtaatgcagc aggaactatt gttttgttag gattttccga agtgtcttgt gagactcctg
    11101 cagtggcttg ctgaattgga tccccaccgt actcgtcaat tccaagggca tcggtaaaca
    11161 tctgctcaaa ctcgaagtcg gccatatcca gagcgccgta gggggcggag tcgtgggggg
    11221 taaatcccgg acccggggaa tccccgtccc ccaacatgtc cagatcgaaa tcgtctagcg
    11281 cgtcggcatg cgccatcgcc acgtcctcgc cgtctaagtg gagctcgtcc cccaggctga
    11341 catcggtcgg gggggcgctc gacctaggaa aaatcagtag aaatagctgt tccagtcttt
    11401 ctagccttga ttccacttct gtcagatgtg ccctagtcag cggagacctt ttggttttgg
    11461 gagagtagcg acactcccag ttgttcttca gacacttggc gcacttcggt ttttctttgg
    11521 agcacttgag ctttttaagt cggcaaatat cgcatgcttg ttcgatagaa gacagtagct
    11581 tcattggatc tgccattgtt taaactagag tcccccgtgt tctctccaaa tgaaatgaac
    11641 ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
    11701 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
    11761 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
    11821 caacgatggc ctttccttta tcgcaatgat ggcatttgta ggagccacct tccttttcca
    11881 ctatcttcac aataaagtga cagatagctg ggcaatggaa tccgaggagg tttccggata
    11941 ttaccctttg ttgaaaagtc tcaattgccc tttggtcttc tgagactgta tctttgatat
    12001 ttttggagta gacaagtgtg tcgtgctcca ccatgttgac gaagattttc ttcttgtcat
    12061 tgagtcgtaa gagactctgt atgaactgtt cgccagtctt tacggcgagt tctgttaggt
    12121 cctctatttg aatctttgac tccatggcct ttgattcagt gggaactacc tttttagaga
    12181 ctccaatctc tattacttgc cttggtttgt gaagcaagcc ttgaatcgtc catactggaa
    12241 tagtacttct gatcttgaga aatatatctt tctctgtgtt cttgatgcag ttagtcctga
    12301 atcttttgac tgcatcttta accttcttgg gaaggtattt gatttcctgg agattattgc
    12361 tcgggtagat cgtcttgatg agacctgctg cgtaagcctc tctaaccatc tgtgggttag
    12421 cattctttct gaaattgaaa aggctaatct ggggacctgc aggaaactat cagtgtttga
    12481 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
    12541 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
    12601 ttccccagat ctggcgccgg ccagcgagac gagcaagatt ggccgccgcc cgaaacgatc
    12661 cgacagcgcg cccagcacag gtgcgcaggc aaattgcacc aacgcataca gcgccagcag
    12721 aatgccatag tgggcggtga cgtcgttcga gtgaaccaga tcgcgcagga ggcccggcag
    12781 caccggcata atcaggccga tgccgacagc gtcgagcgcg acagtgctca gaattacgat
    12841 caggggtatg ttgggtttca cgtctggcct ccggaccagc ctccgctggt ccgattgaac
    12901 gcgcggattc tttatcactg ataagttggt ggacatatta tgtttatcag tgataaagtg
    12961 tcaagcatga caaagttgca gccgaataca gtgatccgtg ccgccctgga cctgttgaac
    13021 gaggtcggcg tagacggtct gacgacacgc aaactggcgg aacggttggg ggttcagcag
    13081 ccggcgcttt actggcactt caggaacaag cgggcgctgc tcgacgcact ggccgaagcc
    13141 atgctggcgg agaatcatac gcattcggtg ccgagagccg acgacgactg gcgctcattt
    13201 ctgatcggga atgcccgcag cttcaggcag gcgctgctcg cctaccgcga tggcgcgcgc
    13261 atccatgccg gcacgcgacc gggcgcaccg cagatggaaa cggccgacgc gcagcttcgc
    13321 ttcctctgcg aggcgggttt ttcggccggg gacgccgtca atgcgctgat gacaatcagc
    13381 tacttcactg ttggggccgt gcttgaggag caggccggcg acagcgatgc cggcgagcgc
    13441 ggcggcaccg ttgaacaggc tccgctctcg ccgctgttgc gggccgcgat agacgccttc
    13501 gacgaagccg gtccggacgc agcgttcgag cagggactcg cggtgattgt cgatggattg
    13561 gcgaaaagga ggctcgttgt caggaacgtt gaaggaccga gaaagggtga cgattgatca
    13621 ggaccgctgc cggagcgcaa cccactcact acagcagagc catgtagaca acatcccctc
    13681 cccctttcca ccgcgtcaga cgcccgtagc agcccgctac gggctttttc atgccctgcc
    13741 ctagcgtcca agcctcacgg ccgcgctcgg cctctctggc ggccttctgg cgctcttccg
    13801 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    13861 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    13921 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    13981 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    14041 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    14101 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    14161 cgcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
    14221 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
    14281 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
    14341 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
    14401 tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca
    14461 accaggaagg gcagcccacc tatcaaggtg tactgccttc cagacgaacg aagagcgatt
    14521 gaggaaaagg cggcggcggc cggcatgagc ctgtcggcct acctgctggc cgtcggccag
    14581 ggctacaaaa tcacgggcgt cgtggactat gagcacgtcc gcgagctggc ccgcatcaat
    14641 ggcgacctgg gccgcctggg cggcctgctg aaactctggc tcaccgacga cccgcgcacg
    14701 gcgcggttcg gtgatgccac gatcctcgcc ctgctggcga agatcgaaga gaagcaggac
    14761 gagcttggca aggtcatgat gggcgtggtc cgcccgaggg cagagccatg acttttttag
    14821 ccgctaaaac ggccgggggg tgcgcgtgat tgccaagcac gtccccatgc gctccatcaa
    14881 gaagagcgac ttcgcggagc tggtgaagta catcaccgac gagcaaggca agaccga
//
