LOCUS       sequence-471104-       10813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast two-hybrid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Sung YC, Li Y, Bernasconi Z, Baik S, Asuke S, Keller B, Fahima T, 
            Coaker G
  TITLE     Wheat tandem kinase RWT4 directly binds a fungal effector to 
            activate defense.
  JOURNAL   Nat Genet. 2025 Apr 14. doi: 10.1038/s41588-025-02162-w.
  PUBMED    40229601
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 Apr 14. doi: 10.1038/s41588-025-02162-w."
COMMENT     Sequence Label: pGADT7-RWT4Ca
FEATURES             Location/Qualifiers
     source          1..10813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1204..1222
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1260..1282)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1382..1401
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      1418..2582
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        3356..4060
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4106..4126
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4142..4483
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     4489..4508
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4489..4507
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4514..4523
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4526..4552
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4559..4583
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7340..7405
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(7412..7436)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      7826..8013
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7881..7905)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(8130..9224)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(9225..9630)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(9672..9688)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9672..9688)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9685..9707)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9696..9712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9720..9726,9727..9744,9745..9750))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9765..9786
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    9841..9874
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    9952..9985
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(10054..10071)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10225..10813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10305..10324)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     1201 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     1261 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     1321 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     1381 gcggcatcag agcagattgt actgagagtg caccataacg catttaagca taaacacgca
     1441 ctatgccgtt cttctcatgt atatatatat acaggcaaca cgcagatata ggtgcgacgt
     1501 gaacagtgag ctgtatgtgc gcagctcgcg ttgcattttc ggaagcgctc gttttcggaa
     1561 acgctttgaa gttcctattc cgaagttcct attctctagc tagaaagtat aggaacttca
     1621 gagcgctttt gaaaaccaaa agcgctctga agacgcactt tcaaaaaacc aaaaacgcac
     1681 cggactgtaa cgagctacta aaatattgcg aataccgctt ccacaaacat tgctcaaaag
     1741 tatctctttg ctatatatct ctgtgctata tccctatata acctacccat ccacctttcg
     1801 ctccttgaac ttgcatctaa actcgacctc tacatttttt atgtttatct ctagtattac
     1861 tctttagaca aaaaaattgt agtaagaact attcatagag tgaatcgaaa acaatacgaa
     1921 aatgtaaaca tttcctatac gtagtatata gagacaaaat agaagaaacc gttcataatt
     1981 ttctgaccaa tgaagaatca tcaacgctat cactttctgt tcacaaagta tgcgcaatcc
     2041 acatcggtat agaatataat cggggatgcc tttatcttga aaaaatgcac ccgcagcttc
     2101 gctagtaatc agtaaacgcg ggaagtggag tcaggctttt tttatggaag agaaaataga
     2161 caccaaagta gccttcttct aaccttaacg gacctacagt gcaaaaagtt atcaagagac
     2221 tgcattatag agcgcacaaa ggagaaaaaa agtaatctaa gatgctttgt tagaaaaata
     2281 gcgctctcgg gatgcatttt tgtagaacaa aaaagaagta tagattcttt gttggtaaaa
     2341 tagcgctctc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa
     2401 aattagcgct ctcgcgttgc atttttgttt tacaaaaatg aagcacagat tcttcgttgg
     2461 taaaatagcg ctttcgcgtt gcatttctgt tctgtaaaaa tgcagctcag attctttgtt
     2521 tgaaaaatta gcgctctcgc gttgcatttt tgttctacaa aatgaagcac agatgcttcg
     2581 ttgcttgcat gcctgcaggt cgagatccgg gatcgaagaa atgatggtaa atgaaatagg
     2641 aaatcaagga gcatgaaggc aaaagacaaa tataagggtc gaacgaaaaa taaagtgaaa
     2701 agtgttgata tgatgtattt ggctttgcgg cgccgaaaaa acgagtttac gcaattgcac
     2761 aatcatgctg actctgtggc ggacccgcgc tcttgccggc ccggcgataa cgctgggcgt
     2821 gaggctgtgc ccggcggagt tttttgcgcc tgcattttcc aaggtttacc ctgcgctaag
     2881 gggcgagatt ggagaagcaa taagaatgcc ggttggggtt gcgatgatga cgaccacgac
     2941 aactggtgtc attatttaag ttgccgaaag aacctgagtg catttgcaac atgagtatac
     3001 tagaagaatg agccaagact tgcgagacgc gagtttgccg gtggtgcgaa caatagagcg
     3061 accatgacct tgaaggtgag acgcgcataa ccgctagagt actttgaaga ggaaacagca
     3121 atagggttgc taccagtata aatagacagg tacatacaac actggaaatg gttgtctgtt
     3181 tgagtacgct ttcaattcat ttgggtgtgc actttattat gttacaatat ggaagggaac
     3241 tttacacttc tcctatgcac atatattaat taaagtccaa tgctagtaga gaaggggggt
     3301 aacacccctc cgcgctcttt tccgattttt ttctaaaccg tggaatattt cggatatcct
     3361 tttgttgttt ccgggtgtac aatatggact tcctcttttc tggcaaccaa acccatacat
     3421 cgggattcct ataatacctt cgttggtctc cctaacatgt aggtggcgga ggggagatat
     3481 acaatagaac agataccaga caagacataa tgggctaaac aagactacac caattacact
     3541 gcctcattga tggtggtaca taacgaacta atactgtagc cctagacttg atagccatca
     3601 tcatatcgaa gtttcactac cctttttcca tttgccatct attgaagtaa taataggcgc
     3661 atgcaacttc ttttcttttt ttttcttttc tctctccccc gttgttgtct caccatatcc
     3721 gcaatgacaa aaaaatgatg gaagacacta aaggaaaaaa ttaacgacaa agacagcacc
     3781 aacagatgtc gttgttccag agctgatgag gggtatctcg aagcacacga aactttttcc
     3841 ttccttcatt cacgcacact actctctaat gagcaacggt atacggcctt ccttccagtt
     3901 acttgaattt gaaataaaaa aaagtttgct gtcttgctat caagtataaa tagacctgca
     3961 attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt gtttcttttt
     4021 ctgcacaata tttcaagcta taccaagcat acaatcaact ccaagctttg caaagatgga
     4081 taaagcggaa ttaattcccg agcctccaaa aaagaagaga aaggtcgaat tgggtaccgc
     4141 cgccaatttt aatcaaagtg ggaatattgc tgatagctca ttgtccttca ctttcactaa
     4201 cagtagcaac ggtccgaacc tcataacaac tcaaacaaat tctcaagcgc tttcacaacc
     4261 aattgcctcc tctaacgttc atgataactt catgaataat gaaatcacgg ctagtaaaat
     4321 tgatgatggt aataattcaa aaccactgtc acctggttgg acggaccaaa ctgcgtataa
     4381 cgcgtttgga atcactacag ggatgtttaa taccactaca atggatgatg tatataacta
     4441 tctattcgat gatgaagata ccccaccaaa cccaaaaaaa gagatcttta atacgactca
     4501 ctatagggcg agcgccgcca tggagtaccc atacgacgta ccagattacg ctcatcaaac
     4561 aagtttgtac aaaaaagcag gctccaccat gatgggtggc tatgagttcc agagggcaga
     4621 attggacgcc cttgagggtg tagttcgtga cccaaccgcc gaacccatgt ctttaacact
     4681 acctcttttg cgtcacatta cgaatgattt ctcccctgag ttcgagatat ctaaggacga
     4741 ctccgccgtg gtatatctag gagtgctacc atcaggattt agggtggcag tgaagaaatc
     4801 acattttagg ttttgtctgg acgacgagga cgccttcact aatgaagttt caattgcaat
     4861 gaaagccgca cataagaata ctgtaagagt aatcggctat tgtcatcata cacatgagca
     4921 gattgccgag tatgagggaa agcaagtctt cgcagaagtg cgtgagaggt tgatatgtac
     4981 tgagtacgtg cccaatggac ctctatccgg ccatatcgaa ggcaaaattt gtgcacaaat
     5041 ggacggctac gagttccagc gtgctgagct tgatgcattg gagagagttg ttagagatac
     5101 ctctgccgag ccaatgtcct tgacactgcc ccttttgcgt catatcacaa atgatttctc
     5161 agatgaatcc aggatcggac gtggaggatt cgctgttgtc tatcttggtg ttttgccatc
     5221 cggcctgaga atagctgtta agaggttgtc aaatatagct tatatgaatg aaagtgcatt
     5281 ccagaacgaa gtattcatca caatgaaagc cacacataaa aatacagtga gattcatggg
     5341 atactgctca cagattcaag gcaaactaat agaacatgat ggacaacatg tctttgcaca
     5401 gcttgaagag aggctaattt gtgtggaata cgcacctaag ggaacattag acgctcacat
     5461 cggagattat ggtgaactgg actggaacca aagatatcag attctgaaag gcatatgtca
     5521 gggtttacat catctacatg acgaaatgca tgtctttcat ggagatatca aacccgccaa
     5581 cattcttatc ggcgataacc tagttcctaa aatctatgat ttcggattga gtcaaatgtt
     5641 cgaggaggag gaaacggaaa gaattgtgga aaacattgcc ggtacattcg gctacatggc
     5701 acctgagttc tgtacaaaca acatggtatc cttcaaggcc gagatttaca gtttaggcgt
     5761 tgtaatagga gaacttctta tcggtaaaaa aggatggttt gacgaggacg tgcggaagct
     5821 ttttgttcag cagttgaaag gccttcgcaa aactttggtg aaggaaggcg cgttttccag
     5881 ctgggagaat aaataccatc aagtgcgcac gtgtatggag attggtcagg attgcatcga
     5941 tccaaatcct cataaacggc ctacgcttct cgaaattatt caacgcctta acgaagccga
     6001 agacatgaat tatagtgcgg catcactctg gcaggttcag tctggtgatg aagaatcaga
     6061 tctctccgat actgaagcgt tggagacaga gaccacgagc gagtttcttc cgtccgatga
     6121 agaaccggcg tccgtcggta agaccggcga gacctctaca caggaaccgg acaaacccga
     6181 tcttatatct aaacttcccg cttcggttga cctttctgat ctcaaggtgc ttgaaaagat
     6241 aaccgatgat tttagccatg agagaatagt cgggaaagac gggactttta aaggatgcca
     6301 taaggctttc gtttataaag gtgacattcc tctgagggag atgattgcag ttaaacggct
     6361 cattggtgtt gagataccct tcgagaagtt caagagagag gctgaacaat tcatcagttt
     6421 ggatcataag aacatagtta aggttgcaag ttattgccac gaccagagcc ggggacatag
     6481 actcgtccag ttcaagggga agcctctgcc ccaacttttt aatgggcctg agcaactgtt
     6541 gtgttacgag tacatgcaca atggttctct tagagattac ttgatgggcc aaggctcccg
     6601 ggtcatagat tggcagatgc ggtacaagct gataaaaggg acctgcgcag gcttgcatta
     6661 tcttcataag ggtcgcgcag gatgtccgat cgttcacctt aatctgagtc ccagcaacgt
     6721 gcttttggat cataattaca ttccgcggat aactggcttc gacttttcca agcttatagg
     6781 agaaaaaaac actaaatctg tggtcttgaa gctcaatgga cccatcgctt atctgccccc
     6841 cgacttcttc cacagcaaag gtacagatct caagtacctg gcgacagttg atatatactc
     6901 tttgggcctg atgatactcg agattgcaac acagcaggaa atcaagggca tacatggtgt
     6961 tctgataaag tcaatcgagg aaaactggcg ggaagagtcc caaatcacac gcctctatac
     7021 ctctcttggg gctgatgagc tgaggcaggt gaagatgtgt attgatatag gacttgactg
     7081 tgtcaagagt aacccagaga agcgccccac tgcaggagca attatgttgt ggcttgataa
     7141 agaatctaag ccagtccccg tgtcacgggc cggagcgggc gttttgccac ggcccccagt
     7201 tccgaccaac atcaaccatg cggggcgcat ccagggtatc ccaatcgaga aatcacaagt
     7261 ccttattcct tcattcgctt gtaatgaata tagctctgtc aaaattcaaa atttgcactt
     7321 gctgctcttt atgggtagcg actacaagga ccacgacggt gactacaagg accacgacat
     7381 cgactacaag gacgacgacg acaagatcta gacccagctt tcttgtacaa agtggttcga
     7441 tcgagctgca gatgaatcgt agatactgaa aaaccccgca agttcacttc aactgtgcat
     7501 cgtgcaccat ctcaatttct ttcatttata catcgttttg ccttctttta tgtaactata
     7561 ctcctctaag tttcaatctt ggccatgtaa cctctgatct atagaatttt ttaaatgact
     7621 agaattaatg cccatctttt ttttggacct aaattcttca tgaaaatata ttacgagggc
     7681 ttattcagaa gctttggact tcttcgccag aggtttggtc aagtctccaa tcaaggttgt
     7741 cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa tcgttggtag
     7801 atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt ttattattaa
     7861 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
     7921 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca
     7981 tgaggtcgct cttattgacc acacctctac cggccggtcg aaattcccct accctatgaa
     8041 catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat aaagtttatg
     8101 tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac ttcttcggcg
     8161 acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt tctgatacct
     8221 gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg caagttcaat
     8281 gacaatttca acatcattgc agcagacaag atagtggcga tagggttgac cttattcttt
     8341 ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt gttcttgtct
     8401 ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat aacggaggct
     8461 tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt taggtgggtt
     8521 gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac cttcaatgta
     8581 ggaaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga agaggccaaa
     8641 acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag ggccatgaaa
     8701 gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact atcccaagcg
     8761 acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac taattctctg
     8821 acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa gtctaaaaga
     8881 gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc tttacggatt
     8941 tttagtaaac cttgttcagg tctaacacta cctgtacccc atttaggacc acccacagca
     9001 cctaacaaaa cggcatcagc cttcttggag gcttccagcg cctcatctgg aagtgggaca
     9061 cctgtagctt cgatagcagc accaccaatt aaatgatttt cgaaatcgaa cttgacattg
     9121 gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat ttcttgacca
     9181 acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat ggtatatcct
     9241 tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa agtaagacga
     9301 ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca acttcaagta
     9361 ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag ccggttccgg
     9421 cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg
     9481 acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg atagcgcccc
     9541 tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc gaactcttgc
     9601 atcttacgat acctgagtat tcccacagtt gggggatctc gactctagct agaggatcaa
     9661 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9721 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9781 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9841 ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag tttacgtcca
     9901 gccaagctag cttggctgca ggtcgagcgg ccgcgatccg gaacccttaa tataacttcg
     9961 tataatgtat gctatacgaa gttatcagct gcattaatga atcggccaac gcgcggggag
    10021 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10081 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10141 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10201 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    10261 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    10321 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    10381 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    10441 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    10501 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10561 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    10621 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    10681 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    10741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    10801 aaaaggatct caa
//
